Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats
Background: Normal exercise can improve human physical abilities, but strenuous exercise can damage human cells. Strenuous exercise causes oxidative stress to the body. In order to determine the level of oxidative stress, it is important to check the levels of Superoxide Dismutase (SOD) and Malondia...
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| Format: | Article |
| Language: | English |
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Kerman University of Medical Sciences
2021-11-01
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| Series: | Journal of Kerman University of Medical Sciences |
| Subjects: | |
| Online Access: | https://jkmu.kmu.ac.ir/article_91825_5d4986acdeb562342982c738c2d6b26c.pdf |
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| author | Mahendra Wahyu Dewangga Irianto Djoko Pekik Dimyati Dimyati Sumaryanto Sumaryanto Nasihun Taufiqqurachman Febrianta Yudha Wahyuni Wahyuni Wijianto Wijianto Agustiyawan Agustiyawan |
| author_facet | Mahendra Wahyu Dewangga Irianto Djoko Pekik Dimyati Dimyati Sumaryanto Sumaryanto Nasihun Taufiqqurachman Febrianta Yudha Wahyuni Wahyuni Wijianto Wijianto Agustiyawan Agustiyawan |
| author_sort | Mahendra Wahyu Dewangga |
| collection | DOAJ |
| description | Background: Normal exercise can improve human physical abilities, but strenuous exercise can damage human cells. Strenuous exercise causes oxidative stress to the body. In order to determine the level of oxidative stress, it is important to check the levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) in the body. SOD is the first line of defence in fighting against the oxidative stress, whereas MDA is the result of oxidative stress cell damage in the body. The sperm cell is the one that is affected by oxidative stress. This research aimed to investigate the differences in the effects of acute and chronic strenuous exercise on SOD production, MDA, and sperm quality.Methods: The research was based on experimental design with post-test only control group design with Wistar rats. Eighteen male Wistar rats were randomly divided into 3 groups (n = 6). Group I: Normal control, Group II: Rats were treated to swim for about 25-40 minutes until they were drowning for 1 day (acute strenuous physical exercise), and Group III: Rats were treated to swim for about 25-40 minutes until they were drowning for almost every day for 2 weeks (chronic strenuous physical exercise). Examination of SOD and MDA levels was done using spectrophotometry, examination of sperm quality was done by looking at the morphology, motility, and sperm quantity through the light microscope at x1000 with haemocytometer.Results: Chronic strenuous exercise significantly affects the decreasing SOD levels, increasing MDA levels, and decreasing sperm quality compared to the control group and acute strenuous exercise (P < 0.05).Conclusion: According to the results of this study, the chronic strenuous exercise effects increase oxidative stress and sperm damage. |
| first_indexed | 2024-03-13T02:09:14Z |
| format | Article |
| id | doaj.art-883387dcacd346ceb879b3b1e34ef27b |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:09:14Z |
| publishDate | 2021-11-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-883387dcacd346ceb879b3b1e34ef27b2023-07-01T05:12:02ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432021-11-0128653954710.22062/jkmu.2021.9182591825Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar RatsMahendra Wahyu Dewangga0Irianto Djoko Pekik1Dimyati Dimyati2Sumaryanto Sumaryanto3Nasihun Taufiqqurachman4Febrianta Yudha5Wahyuni Wahyuni6Wijianto Wijianto7Agustiyawan Agustiyawan8Department of Sport Science, Faculty of Sport Science, Universitas Negeri Yogyakarta, Yogyakarta, IndonesiaDepartment of Sport Science, Faculty of Sport Science, Universitas Negeri Yogyakarta, Yogyakarta, IndonesiaDepartment of Sport Science, Faculty of Sport Science, Universitas Negeri Yogyakarta, Yogyakarta, IndonesiaDepartment of Sport Science, Faculty of Sport Science, Universitas Negeri Yogyakarta, Yogyakarta, IndonesiaDepartment Biomedical Science, Faculty of Medicine, Universitas Islam Sultan Agung Semarang, Jawa Tengah, IndonesiaDepartment of Education Primary School Teachers, Faculty of Education, Universitas Muhammadiyah Purwokerto, Jawa Tengah, IndonesiaDepartment of Physiotherapy, Faculty of Health Science, Universitas Muhammadiyah Surakarta, Jawa Tengah, IndonesiaDepartment of Physiotherapy, Faculty of Health Science, Universitas Muhammadiyah Surakarta, Jawa Tengah, IndonesiaDepartment of Physiotherapy, Faculty of Health Science, Universitas Pembangunan Nasional Veteran Jakarta, Jakarta, IndonesiaBackground: Normal exercise can improve human physical abilities, but strenuous exercise can damage human cells. Strenuous exercise causes oxidative stress to the body. In order to determine the level of oxidative stress, it is important to check the levels of Superoxide Dismutase (SOD) and Malondialdehyde (MDA) in the body. SOD is the first line of defence in fighting against the oxidative stress, whereas MDA is the result of oxidative stress cell damage in the body. The sperm cell is the one that is affected by oxidative stress. This research aimed to investigate the differences in the effects of acute and chronic strenuous exercise on SOD production, MDA, and sperm quality.Methods: The research was based on experimental design with post-test only control group design with Wistar rats. Eighteen male Wistar rats were randomly divided into 3 groups (n = 6). Group I: Normal control, Group II: Rats were treated to swim for about 25-40 minutes until they were drowning for 1 day (acute strenuous physical exercise), and Group III: Rats were treated to swim for about 25-40 minutes until they were drowning for almost every day for 2 weeks (chronic strenuous physical exercise). Examination of SOD and MDA levels was done using spectrophotometry, examination of sperm quality was done by looking at the morphology, motility, and sperm quantity through the light microscope at x1000 with haemocytometer.Results: Chronic strenuous exercise significantly affects the decreasing SOD levels, increasing MDA levels, and decreasing sperm quality compared to the control group and acute strenuous exercise (P < 0.05).Conclusion: According to the results of this study, the chronic strenuous exercise effects increase oxidative stress and sperm damage.https://jkmu.kmu.ac.ir/article_91825_5d4986acdeb562342982c738c2d6b26c.pdfstrenuous physical exerciseoxidative stresssuperoxide dismutasemalondialdehydesperm qualitysport physiology |
| spellingShingle | Mahendra Wahyu Dewangga Irianto Djoko Pekik Dimyati Dimyati Sumaryanto Sumaryanto Nasihun Taufiqqurachman Febrianta Yudha Wahyuni Wahyuni Wijianto Wijianto Agustiyawan Agustiyawan Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats Journal of Kerman University of Medical Sciences strenuous physical exercise oxidative stress superoxide dismutase malondialdehyde sperm quality sport physiology |
| title | Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats |
| title_full | Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats |
| title_fullStr | Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats |
| title_full_unstemmed | Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats |
| title_short | Different Effects of Acute and Chronic Strenuous Physical Exercise on Superoxide Dismutase (SOD), Malondialdehyde (MDA) Levels, and Sperm Quality of the Wistar Rats |
| title_sort | different effects of acute and chronic strenuous physical exercise on superoxide dismutase sod malondialdehyde mda levels and sperm quality of the wistar rats |
| topic | strenuous physical exercise oxidative stress superoxide dismutase malondialdehyde sperm quality sport physiology |
| url | https://jkmu.kmu.ac.ir/article_91825_5d4986acdeb562342982c738c2d6b26c.pdf |
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