Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp.
Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/11/1515 |
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| author | Wiem Elloumi Ahlem Jebali Amina Maalej Mohamed Chamkha Sami Sayadi |
| author_facet | Wiem Elloumi Ahlem Jebali Amina Maalej Mohamed Chamkha Sami Sayadi |
| author_sort | Wiem Elloumi |
| collection | DOAJ |
| description | Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of <i>Scenedesmus</i> sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in <i>Scenedesmus</i> sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that <i>Scenedesmus</i> sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries. |
| first_indexed | 2024-03-10T15:03:11Z |
| format | Article |
| id | doaj.art-88345b42727e47c4ada5a4350bb24e8a |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T15:03:11Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-88345b42727e47c4ada5a4350bb24e8a2023-11-20T19:58:22ZengMDPI AGBiomolecules2218-273X2020-11-011011151510.3390/biom10111515Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp.Wiem Elloumi0Ahlem Jebali1Amina Maalej2Mohamed Chamkha3Sami Sayadi4Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, TunisiaLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, TunisiaLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, TunisiaLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, P.O. Box 1177, 3018 Sfax, TunisiaCenter for Sustainable Development, College of Arts and Sciences, Qatar University, Doha 2713, QatarCarotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of <i>Scenedesmus</i> sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in <i>Scenedesmus</i> sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that <i>Scenedesmus</i> sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.https://www.mdpi.com/2218-273X/10/11/1515<i>Scenedesmus</i> sp.growthpigmentscarotenoidsantioxidant activitiesenzymatic activities |
| spellingShingle | Wiem Elloumi Ahlem Jebali Amina Maalej Mohamed Chamkha Sami Sayadi Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. Biomolecules <i>Scenedesmus</i> sp. growth pigments carotenoids antioxidant activities enzymatic activities |
| title | Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. |
| title_full | Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. |
| title_fullStr | Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. |
| title_full_unstemmed | Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. |
| title_short | Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae <i>Scenedesmus</i> sp. |
| title_sort | effect of mild salinity stress on the growth fatty acid and carotenoid compositions and biological activities of the thermal freshwater microalgae i scenedesmus i sp |
| topic | <i>Scenedesmus</i> sp. growth pigments carotenoids antioxidant activities enzymatic activities |
| url | https://www.mdpi.com/2218-273X/10/11/1515 |
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