Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
The red flour beetle, Tribolium castaneum, is a pest of stored products. It is also regarded as a model species for studying development, genetics, biology, physiology and biochemistry. Recently, it has become a model for use in RNA interference experiments. 20-hydroxyecdysone (20E) is involved in i...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2018-08-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201801-0042_Effects_of_20-hydroxyecdysone_on_the_development_and_morphology_of_the_red_flour_beetle_Tribolium_castaneum.php |
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| author | Nujira TATUN Phiraya KUMDI Jatuporn TUNGJITWITAYAKUL Sho SAKURAI |
| author_facet | Nujira TATUN Phiraya KUMDI Jatuporn TUNGJITWITAYAKUL Sho SAKURAI |
| author_sort | Nujira TATUN |
| collection | DOAJ |
| description | The red flour beetle, Tribolium castaneum, is a pest of stored products. It is also regarded as a model species for studying development, genetics, biology, physiology and biochemistry. Recently, it has become a model for use in RNA interference experiments. 20-hydroxyecdysone (20E) is involved in insect metamorphosis and its role in organ development in T. castaneum are based on hormonal treatment in conjunction with RNAi. However, information on the biological, morphological and physiological effects of 20E on T. castaneum is still limited. This study reveals the responses of T. castaneum larvae to injections with various doses of 20E (100, 200, 300, 400 and 500 ng / insect). The results show that larvae injected with 20E reached the prepupal, pupal and adult stages earlier than the control group. Different degrees of morphological change were observed in nine traits, including the appearance of pupal prothetelic organs in the larvae. Moreover, an injection of a high dose of 20E reduced the body weights of the resulting insects at each stage, as well as the length and width of elytra. The enzymatic activity of α-amylase in the resulting adults also decreased significantly. This indicates that injection of 20E caused precocious metamorphosis in T. castaneum by inducing changes in morphology and α-amylase activity, and the optimal concentrations that induce such phenomena were in the range of 100-200 ng / insect. Further investigations are needed to examine the roles of 20E in the regulation of α-amylase in T. castaneum. |
| first_indexed | 2024-12-20T04:44:02Z |
| format | Article |
| id | doaj.art-883aea7ad7e44b41bd1e60b6384a252c |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-20T04:44:02Z |
| publishDate | 2018-08-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-883aea7ad7e44b41bd1e60b6384a252c2022-12-21T19:53:02ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292018-08-01115142443110.14411/eje.2018.042eje-201801-0042Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)Nujira TATUN0Phiraya KUMDI1Jatuporn TUNGJITWITAYAKUL2Sho SAKURAI3School of Science, University of Phayao, Phayao 56000, Thailand; e-mails: nujira.ta@up.ac.th, t_jatuporn@hotmail.comSchool of Science, University of Phayao, Phayao 56000, Thailand; e-mails: nujira.ta@up.ac.th, t_jatuporn@hotmail.comSchool of Science, University of Phayao, Phayao 56000, Thailand; e-mails: nujira.ta@up.ac.th, t_jatuporn@hotmail.comDivision of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192, Japan; e-mail: ssakurai@staff.kanazawa-u.ac.jpThe red flour beetle, Tribolium castaneum, is a pest of stored products. It is also regarded as a model species for studying development, genetics, biology, physiology and biochemistry. Recently, it has become a model for use in RNA interference experiments. 20-hydroxyecdysone (20E) is involved in insect metamorphosis and its role in organ development in T. castaneum are based on hormonal treatment in conjunction with RNAi. However, information on the biological, morphological and physiological effects of 20E on T. castaneum is still limited. This study reveals the responses of T. castaneum larvae to injections with various doses of 20E (100, 200, 300, 400 and 500 ng / insect). The results show that larvae injected with 20E reached the prepupal, pupal and adult stages earlier than the control group. Different degrees of morphological change were observed in nine traits, including the appearance of pupal prothetelic organs in the larvae. Moreover, an injection of a high dose of 20E reduced the body weights of the resulting insects at each stage, as well as the length and width of elytra. The enzymatic activity of α-amylase in the resulting adults also decreased significantly. This indicates that injection of 20E caused precocious metamorphosis in T. castaneum by inducing changes in morphology and α-amylase activity, and the optimal concentrations that induce such phenomena were in the range of 100-200 ng / insect. Further investigations are needed to examine the roles of 20E in the regulation of α-amylase in T. castaneum.https://www.eje.cz/artkey/eje-201801-0042_Effects_of_20-hydroxyecdysone_on_the_development_and_morphology_of_the_red_flour_beetle_Tribolium_castaneum.phpcoleopteratenebrionidaetribolium castaneum20-hydroxyecdysonewing growthα-amylaseprothetelic |
| spellingShingle | Nujira TATUN Phiraya KUMDI Jatuporn TUNGJITWITAYAKUL Sho SAKURAI Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) European Journal of Entomology coleoptera tenebrionidae tribolium castaneum 20-hydroxyecdysone wing growth α-amylase prothetelic |
| title | Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_full | Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_fullStr | Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_full_unstemmed | Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_short | Effects of 20-hydroxyecdysone on the development and morphology of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae) |
| title_sort | effects of 20 hydroxyecdysone on the development and morphology of the red flour beetle tribolium castaneum coleoptera tenebrionidae |
| topic | coleoptera tenebrionidae tribolium castaneum 20-hydroxyecdysone wing growth α-amylase prothetelic |
| url | https://www.eje.cz/artkey/eje-201801-0042_Effects_of_20-hydroxyecdysone_on_the_development_and_morphology_of_the_red_flour_beetle_Tribolium_castaneum.php |
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