Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i>
Members of the <i>Borrelia burgdorferi</i> sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and <i>Borrelia miyamotoi</i> is one of the relapsing fever <i>Borrelia</i> (RFB). Despite the serological evidence of LD in Malaysia, there has been...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/10/846 |
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| author | Alice C. C. Lau Yongjin Qiu Mohamed Abdallah Mohamed Moustafa Ryo Nakao Michito Shimozuru Manabu Onuma Jayasilan Mohd-Azlan Toshio Tsubota |
| author_facet | Alice C. C. Lau Yongjin Qiu Mohamed Abdallah Mohamed Moustafa Ryo Nakao Michito Shimozuru Manabu Onuma Jayasilan Mohd-Azlan Toshio Tsubota |
| author_sort | Alice C. C. Lau |
| collection | DOAJ |
| description | Members of the <i>Borrelia burgdorferi</i> sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and <i>Borrelia miyamotoi</i> is one of the relapsing fever <i>Borrelia</i> (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize <i>Borrelia</i> in rodents and <i>Ixodes</i> ticks from primary forests and an oil palm (OP) plantation in Sarawak. <i>Borrelia yangtzensis</i> (a member of the Bbsl complex) was detected in 43.8% (14/32) of <i>Ixodes granulatus</i>; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, <i>B. yangtzensis</i> was detected in four <i>Rattus</i> spp. from the OP plantation and <i>B. miyamotoi</i> was detected in one rodent, <i>Sundamys muelleri</i>, from the primary forest. Further, the positive samples of <i>B. yangtzensis</i> were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of <i>B. miyamotoi</i>, a known human pathogen in Malaysia, and <i>B. yangtzensis</i>, which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the <i>Borrelia</i> spp. |
| first_indexed | 2024-03-10T15:35:38Z |
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| id | doaj.art-883bb9fcec0d44ef86fd67b74b47b3c8 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T15:35:38Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-883bb9fcec0d44ef86fd67b74b47b3c82023-11-20T17:18:00ZengMDPI AGPathogens2076-08172020-10-0191084610.3390/pathogens9100846Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i>Alice C. C. Lau0Yongjin Qiu1Mohamed Abdallah Mohamed Moustafa2Ryo Nakao3Michito Shimozuru4Manabu Onuma5Jayasilan Mohd-Azlan6Toshio Tsubota7Laboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanHokudai Center for Zoonosis Control in Zambia, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, JapanLaboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanLaboratory of Parasitology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanEcological Risk Assessment and Control Section, Center for Environmental Biology and Ecosystem Studies, National Institute for Environmental Studies, Tsukuba 305-806, JapanInstitute of Biodiversity and Environmental Conservation, University Malaysia Sarawak, Kota Samarahan, Sarawak 94300, MalaysiaLaboratory of Wildlife Biology and Medicine, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanMembers of the <i>Borrelia burgdorferi</i> sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and <i>Borrelia miyamotoi</i> is one of the relapsing fever <i>Borrelia</i> (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize <i>Borrelia</i> in rodents and <i>Ixodes</i> ticks from primary forests and an oil palm (OP) plantation in Sarawak. <i>Borrelia yangtzensis</i> (a member of the Bbsl complex) was detected in 43.8% (14/32) of <i>Ixodes granulatus</i>; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, <i>B. yangtzensis</i> was detected in four <i>Rattus</i> spp. from the OP plantation and <i>B. miyamotoi</i> was detected in one rodent, <i>Sundamys muelleri</i>, from the primary forest. Further, the positive samples of <i>B. yangtzensis</i> were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of <i>B. miyamotoi</i>, a known human pathogen in Malaysia, and <i>B. yangtzensis</i>, which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the <i>Borrelia</i> spp.https://www.mdpi.com/2076-0817/9/10/846<i>Borrelia miyamotoi</i><i>Borrelia yangtzensis</i><i>Ixodes granulatus</i>rodentSarawakMalaysia |
| spellingShingle | Alice C. C. Lau Yongjin Qiu Mohamed Abdallah Mohamed Moustafa Ryo Nakao Michito Shimozuru Manabu Onuma Jayasilan Mohd-Azlan Toshio Tsubota Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> Pathogens <i>Borrelia miyamotoi</i> <i>Borrelia yangtzensis</i> <i>Ixodes granulatus</i> rodent Sarawak Malaysia |
| title | Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> |
| title_full | Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> |
| title_fullStr | Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> |
| title_full_unstemmed | Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> |
| title_short | Detection of <i>Borrelia</i> <i>burgdorferi</i> Sensu Lato and Relapsing Fever <i>Borrelia</i> in Feeding <i>Ixodes</i> Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of <i>Borrelia yangtzensis</i> and <i>Borrelia miyamotoi</i> |
| title_sort | detection of i borrelia i i burgdorferi i sensu lato and relapsing fever i borrelia i in feeding i ixodes i ticks and rodents in sarawak malaysia new geographical records of i borrelia yangtzensis i and i borrelia miyamotoi i |
| topic | <i>Borrelia miyamotoi</i> <i>Borrelia yangtzensis</i> <i>Ixodes granulatus</i> rodent Sarawak Malaysia |
| url | https://www.mdpi.com/2076-0817/9/10/846 |
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