SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed
Brassinosteroids (BRs) are required for various aspects of plant growth and development, but also participate in stress responses. The hormones convey their activity through transcriptional regulation and posttranslational modification of transcription factors and one class are basic helix-loop-heli...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.930805/full |
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| author | Haiwei Shuai Tingting Chen Tanja Wlk Wilfried Rozhon Maria J. Pimenta Lange Tobias Sieberer Theo Lange Brigitte Poppenberger |
| author_facet | Haiwei Shuai Tingting Chen Tanja Wlk Wilfried Rozhon Maria J. Pimenta Lange Tobias Sieberer Theo Lange Brigitte Poppenberger |
| author_sort | Haiwei Shuai |
| collection | DOAJ |
| description | Brassinosteroids (BRs) are required for various aspects of plant growth and development, but also participate in stress responses. The hormones convey their activity through transcriptional regulation and posttranslational modification of transcription factors and one class are basic helix-loop-helix (bHLH) proteins of the BR Enhanced Expression (BEE) subfamily, which in Arabidopsis thaliana include BEE1-3 and CESTA (CES). CES and the BEEs promote the expression of different BR-responsive genes, including genes encoding gibberellin (GA) biosynthetic and catabolizing enzymes, as well as cold-responsive genes. Interestingly, in terms of an application, CES could promote both fruit growth and cold stress tolerance when over-expressed in A. thaliana and here it was investigated, if this function is conserved in the fruit crop Solanum lycopersicum (cultivated tomato). Based on amino acid sequence similarity and the presence of regulatory motifs, a CES orthologue of S. lycopersicum, SlCES, was identified and the effects of its over-expression were analysed in tomato. This showed that SlCES, like AtCES, was re-localized to nuclear bodies in response to BR signaling activation and that it effected GA homeostasis, with related phenotypes, when over-expressed. In addition, over-expression lines showed an increased chilling tolerance and had altered fruit characteristics. The possibilities and potential limitations of a gain of SlCES function as a breeding strategy for tomato are discussed. |
| first_indexed | 2024-12-12T02:19:34Z |
| format | Article |
| id | doaj.art-8841b0ba373845cea07cfcd32f4323f7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T02:19:34Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8841b0ba373845cea07cfcd32f4323f72022-12-22T00:41:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.930805930805SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-ExpressedHaiwei Shuai0Tingting Chen1Tanja Wlk2Wilfried Rozhon3Maria J. Pimenta Lange4Tobias Sieberer5Theo Lange6Brigitte Poppenberger7Biotechnology of Horticultural Crops, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyBiotechnology of Horticultural Crops, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyBiotechnology of Horticultural Crops, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyBiotechnology of Horticultural Crops, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyInstitute of Plant Biology, Technical University of Braunschweig, Braunschweig, GermanyPlant Growth Regulation, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyInstitute of Plant Biology, Technical University of Braunschweig, Braunschweig, GermanyBiotechnology of Horticultural Crops, TUM School of Life Sciences, Technical University of Munich, Freising, GermanyBrassinosteroids (BRs) are required for various aspects of plant growth and development, but also participate in stress responses. The hormones convey their activity through transcriptional regulation and posttranslational modification of transcription factors and one class are basic helix-loop-helix (bHLH) proteins of the BR Enhanced Expression (BEE) subfamily, which in Arabidopsis thaliana include BEE1-3 and CESTA (CES). CES and the BEEs promote the expression of different BR-responsive genes, including genes encoding gibberellin (GA) biosynthetic and catabolizing enzymes, as well as cold-responsive genes. Interestingly, in terms of an application, CES could promote both fruit growth and cold stress tolerance when over-expressed in A. thaliana and here it was investigated, if this function is conserved in the fruit crop Solanum lycopersicum (cultivated tomato). Based on amino acid sequence similarity and the presence of regulatory motifs, a CES orthologue of S. lycopersicum, SlCES, was identified and the effects of its over-expression were analysed in tomato. This showed that SlCES, like AtCES, was re-localized to nuclear bodies in response to BR signaling activation and that it effected GA homeostasis, with related phenotypes, when over-expressed. In addition, over-expression lines showed an increased chilling tolerance and had altered fruit characteristics. The possibilities and potential limitations of a gain of SlCES function as a breeding strategy for tomato are discussed.https://www.frontiersin.org/articles/10.3389/fpls.2022.930805/fullBEEsbrassinosteroidsgibberellinshormonesSolanumSUMOylation |
| spellingShingle | Haiwei Shuai Tingting Chen Tanja Wlk Wilfried Rozhon Maria J. Pimenta Lange Tobias Sieberer Theo Lange Brigitte Poppenberger SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed Frontiers in Plant Science BEEs brassinosteroids gibberellins hormones Solanum SUMOylation |
| title | SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed |
| title_full | SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed |
| title_fullStr | SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed |
| title_full_unstemmed | SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed |
| title_short | SlCESTA Is a Brassinosteroid-Regulated bHLH Transcription Factor of Tomato That Promotes Chilling Tolerance and Fruit Growth When Over-Expressed |
| title_sort | slcesta is a brassinosteroid regulated bhlh transcription factor of tomato that promotes chilling tolerance and fruit growth when over expressed |
| topic | BEEs brassinosteroids gibberellins hormones Solanum SUMOylation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.930805/full |
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