Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata
Cyclea barbata has been reported as traditional food and medicinal plant. Although its morphology resembles Cissampelos pareira, Cyclea polypetala and Stephania japonica very closely, chemical compositions of them are significantly different. Here we showed the capability of a short ITS region (27...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2020-12-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/42-6/4.pdf |
| _version_ | 1818891615173869568 |
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| author | Kun Silprasit Sirikul Thummajitsakul |
| author_facet | Kun Silprasit Sirikul Thummajitsakul |
| author_sort | Kun Silprasit |
| collection | DOAJ |
| description | Cyclea barbata has been reported as traditional food and medicinal plant. Although its morphology resembles
Cissampelos pareira, Cyclea polypetala and Stephania japonica very closely, chemical compositions of them are significantly
different. Here we showed the capability of a short ITS region (277 nucleotides) for C. barbata identification. The partial ITS and
rbcL regions from 14 samples, collected in many areas of Thailand, were amplified. The results after DNA blast on NCBI
showed that all short ITS sequences closely related to C. barbata. The phylogenic analysis from the ITS and combined ITS/rbcL
data by maximum parsimony and maximum likelihood methods showed that all samples was recognized into C. barbata. The
results of DNA analysis were consistent with morphological identification. Our findings indicated that this short ITS region was
able to distinguish C. barbata from their adulterants. This in turn can reduce cost and time for C. barbata identification using
DNA barcode. |
| first_indexed | 2024-12-19T17:43:37Z |
| format | Article |
| id | doaj.art-8841fac816b847a6a46fd620e65017eb |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-19T17:43:37Z |
| publishDate | 2020-12-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-8841fac816b847a6a46fd620e65017eb2022-12-21T20:12:08ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952020-12-014261197120610.14456/sjst-psu.2020.155Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbataKun Silprasit0Sirikul Thummajitsakul1Department of Environment, Faculty of Environmental Culture and Ecotourism, Srinakharinwirot University, Watthana, Bangkok, 10110 ThailandDepartment of Health Promotion, Faculty of Physical Therapy, Srinakharinwirot University, Ongkharak, Nakhon Nayok, 26120 ThailandCyclea barbata has been reported as traditional food and medicinal plant. Although its morphology resembles Cissampelos pareira, Cyclea polypetala and Stephania japonica very closely, chemical compositions of them are significantly different. Here we showed the capability of a short ITS region (277 nucleotides) for C. barbata identification. The partial ITS and rbcL regions from 14 samples, collected in many areas of Thailand, were amplified. The results after DNA blast on NCBI showed that all short ITS sequences closely related to C. barbata. The phylogenic analysis from the ITS and combined ITS/rbcL data by maximum parsimony and maximum likelihood methods showed that all samples was recognized into C. barbata. The results of DNA analysis were consistent with morphological identification. Our findings indicated that this short ITS region was able to distinguish C. barbata from their adulterants. This in turn can reduce cost and time for C. barbata identification using DNA barcode.https://rdo.psu.ac.th/sjstweb/journal/42-6/4.pdfmoonseed familyphylogenic treedna barcodemorphologycyclea barbata |
| spellingShingle | Kun Silprasit Sirikul Thummajitsakul Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata Songklanakarin Journal of Science and Technology (SJST) moonseed family phylogenic tree dna barcode morphology cyclea barbata |
| title | Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata |
| title_full | Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata |
| title_fullStr | Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata |
| title_full_unstemmed | Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata |
| title_short | Short ITS DNA barcode effectively distinguishes the medicinal plants Cyclea barbata |
| title_sort | short its dna barcode effectively distinguishes the medicinal plants cyclea barbata |
| topic | moonseed family phylogenic tree dna barcode morphology cyclea barbata |
| url | https://rdo.psu.ac.th/sjstweb/journal/42-6/4.pdf |
| work_keys_str_mv | AT kunsilprasit shortitsdnabarcodeeffectivelydistinguishesthemedicinalplantscycleabarbata AT sirikulthummajitsakul shortitsdnabarcodeeffectivelydistinguishesthemedicinalplantscycleabarbata |