Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development
The obligate biotroph <i>Plasmodiophora brassicae</i> causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the bi...
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MDPI AG
2020-05-01
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| Series: | International Journal of Molecular Sciences |
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| author | Rawnak Laila Arif Hasan Khan Robin Jong-In Park Gopal Saha Hoy-Taek Kim Md. Abdul Kayum Ill-Sup Nou |
| author_facet | Rawnak Laila Arif Hasan Khan Robin Jong-In Park Gopal Saha Hoy-Taek Kim Md. Abdul Kayum Ill-Sup Nou |
| author_sort | Rawnak Laila |
| collection | DOAJ |
| description | The obligate biotroph <i>Plasmodiophora brassicae</i> causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the biosynthesis, signaling, and degradation in Chinese cabbage inoculated with the Korean pathotype group 4 isolate of <i>P. brassicae</i>, Seosan. This isolate produced the most severe clubroot symptoms in Chinese cabbage cultivar “Bullam-3-ho” compared to three other Korean geographical isolates investigated. <i>BrIPT1</i>, a cytokinin biosynthesis gene, was induced on Day 1 and Day 28 in infected root tissues and the upregulation of this biosynthetic gene coincided with the higher expression of the response regulators <i>BrRR1</i>, on both Days and <i>BrRR6</i> on Day 1 and 3. <i>BrRR3</i> and <i>4</i> genes were also induced during gall enlargement on Day 35 in leaf tissues. The <i>BrRR4</i> gene, which positively interact with phytochrome B, was consistently induced in leaf tissues on Day 1, 3, and 14 in the inoculated plants. The cytokinin degrading gene <i>BrCKX3-6</i> were induced on Day 14, before gall initiation. <i>BrCKX2,3,6</i> were induced until Day 28 and their expression was downregulated on Day 35. This insight improves our current understanding of the role of cytokinin signaling genes in clubroot disease development. |
| first_indexed | 2024-03-10T19:29:46Z |
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| language | English |
| last_indexed | 2024-03-10T19:29:46Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8842273ca2fc49c89154aa8babf756272023-11-20T02:14:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012111389610.3390/ijms21113896Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease DevelopmentRawnak Laila0Arif Hasan Khan Robin1Jong-In Park2Gopal Saha3Hoy-Taek Kim4Md. Abdul Kayum5Ill-Sup Nou6Department of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaDepartment of Horticulture, Sunchon National University, Suncheon 57922, KoreaThe obligate biotroph <i>Plasmodiophora brassicae</i> causes clubroot disease in oilseeds and vegetables of the Brassicaceae family, and cytokinins play a vital role in clubroot formation. In this study, we examined the expression patterns of 17 cytokinin-related genes involved in the biosynthesis, signaling, and degradation in Chinese cabbage inoculated with the Korean pathotype group 4 isolate of <i>P. brassicae</i>, Seosan. This isolate produced the most severe clubroot symptoms in Chinese cabbage cultivar “Bullam-3-ho” compared to three other Korean geographical isolates investigated. <i>BrIPT1</i>, a cytokinin biosynthesis gene, was induced on Day 1 and Day 28 in infected root tissues and the upregulation of this biosynthetic gene coincided with the higher expression of the response regulators <i>BrRR1</i>, on both Days and <i>BrRR6</i> on Day 1 and 3. <i>BrRR3</i> and <i>4</i> genes were also induced during gall enlargement on Day 35 in leaf tissues. The <i>BrRR4</i> gene, which positively interact with phytochrome B, was consistently induced in leaf tissues on Day 1, 3, and 14 in the inoculated plants. The cytokinin degrading gene <i>BrCKX3-6</i> were induced on Day 14, before gall initiation. <i>BrCKX2,3,6</i> were induced until Day 28 and their expression was downregulated on Day 35. This insight improves our current understanding of the role of cytokinin signaling genes in clubroot disease development.https://www.mdpi.com/1422-0067/21/11/3896Chinese cabbagecytokininclubroot<i>Plasmodiophora brassicae</i>expression analysis |
| spellingShingle | Rawnak Laila Arif Hasan Khan Robin Jong-In Park Gopal Saha Hoy-Taek Kim Md. Abdul Kayum Ill-Sup Nou Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development International Journal of Molecular Sciences Chinese cabbage cytokinin clubroot <i>Plasmodiophora brassicae</i> expression analysis |
| title | Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development |
| title_full | Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development |
| title_fullStr | Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development |
| title_full_unstemmed | Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development |
| title_short | Expression and Role of Response Regulating, Biosynthetic and Degrading Genes for Cytokinin Signaling during Clubroot Disease Development |
| title_sort | expression and role of response regulating biosynthetic and degrading genes for cytokinin signaling during clubroot disease development |
| topic | Chinese cabbage cytokinin clubroot <i>Plasmodiophora brassicae</i> expression analysis |
| url | https://www.mdpi.com/1422-0067/21/11/3896 |
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