Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease
AbstractBackground:Human tissue kallikrein (hK1) is a key enzyme in the kallikrein–kinin system (KKS). hK1-specific amidase activity is reduced in urine samples from hypertensive and heart failure (HF) patients. The pathophysiologic role of hK1 in coronary artery disease (CAD) remains unclear.Object...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Cardiologia (SBC)
2015-11-01
|
| Series: | Arquivos Brasileiros de Cardiologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015002400457&lng=en&tlng=en |
| _version_ | 1811345315853238272 |
|---|---|
| author | Estêvão Lanna Figueiredo Carolina Antunes Magalhães Karlyse Claudino Belli Ari Mandil José Carlos Faria Garcia Rosanã Aparecida Araújo Amintas Fabiano de Souza Figueiredo Lucia Campos Pellanda |
| author_facet | Estêvão Lanna Figueiredo Carolina Antunes Magalhães Karlyse Claudino Belli Ari Mandil José Carlos Faria Garcia Rosanã Aparecida Araújo Amintas Fabiano de Souza Figueiredo Lucia Campos Pellanda |
| author_sort | Estêvão Lanna Figueiredo |
| collection | DOAJ |
| description | AbstractBackground:Human tissue kallikrein (hK1) is a key enzyme in the kallikrein–kinin system (KKS). hK1-specific amidase activity is reduced in urine samples from hypertensive and heart failure (HF) patients. The pathophysiologic role of hK1 in coronary artery disease (CAD) remains unclear.Objective:To evaluate hK1-specific amidase activity in the urine of CAD patientsMethods:Sixty-five individuals (18–75 years) who underwent cardiac catheterism (CATH) were included. Random midstream urine samples were collected immediately before CATH. Patients were classified in two groups according to the presence of coronary lesions: CAD (43 patients) and non-CAD (22 patients). hK1 amidase activity was estimated using the chromogenic substrate D-Val-Leu-Arg-Nan. Creatinine was determined using Jaffé’s method. Urinary hK1-specific amidase activity was expressed as µM/(min · mg creatinine) to correct for differences in urine flow rates.Results:Urinary hK1-specific amidase activity levels were similar between CAD [0.146 µM/(min ·mg creatinine)] and non-CAD [0.189 µM/(min . mg creatinine)] patients (p = 0.803) and remained similar to values previously reported for hypertensive patients [0.210 µM/(min . mg creatinine)] and HF patients [0.104 µM/(min . mg creatinine)]. CAD severity and hypertension were not observed to significantly affect urinary hK1-specific amidase activity.Conclusion:CAD patients had low levels of urinary hK1-specific amidase activity, suggesting that renal KKS activity may be reduced in patients with this disease. |
| first_indexed | 2024-04-13T20:01:19Z |
| format | Article |
| id | doaj.art-884391ec104d420eb14a27884d23cb9d |
| institution | Directory Open Access Journal |
| issn | 1678-4170 |
| language | English |
| last_indexed | 2024-04-13T20:01:19Z |
| publishDate | 2015-11-01 |
| publisher | Sociedade Brasileira de Cardiologia (SBC) |
| record_format | Article |
| series | Arquivos Brasileiros de Cardiologia |
| spelling | doaj.art-884391ec104d420eb14a27884d23cb9d2022-12-22T02:32:11ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia1678-41702015-11-01105545756510.5935/abc.20150109S0066-782X2015002400457Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery DiseaseEstêvão Lanna FigueiredoCarolina Antunes MagalhãesKarlyse Claudino BelliAri MandilJosé Carlos Faria GarciaRosanã Aparecida AraújoAmintas Fabiano de Souza FigueiredoLucia Campos PellandaAbstractBackground:Human tissue kallikrein (hK1) is a key enzyme in the kallikrein–kinin system (KKS). hK1-specific amidase activity is reduced in urine samples from hypertensive and heart failure (HF) patients. The pathophysiologic role of hK1 in coronary artery disease (CAD) remains unclear.Objective:To evaluate hK1-specific amidase activity in the urine of CAD patientsMethods:Sixty-five individuals (18–75 years) who underwent cardiac catheterism (CATH) were included. Random midstream urine samples were collected immediately before CATH. Patients were classified in two groups according to the presence of coronary lesions: CAD (43 patients) and non-CAD (22 patients). hK1 amidase activity was estimated using the chromogenic substrate D-Val-Leu-Arg-Nan. Creatinine was determined using Jaffé’s method. Urinary hK1-specific amidase activity was expressed as µM/(min · mg creatinine) to correct for differences in urine flow rates.Results:Urinary hK1-specific amidase activity levels were similar between CAD [0.146 µM/(min ·mg creatinine)] and non-CAD [0.189 µM/(min . mg creatinine)] patients (p = 0.803) and remained similar to values previously reported for hypertensive patients [0.210 µM/(min . mg creatinine)] and HF patients [0.104 µM/(min . mg creatinine)]. CAD severity and hypertension were not observed to significantly affect urinary hK1-specific amidase activity.Conclusion:CAD patients had low levels of urinary hK1-specific amidase activity, suggesting that renal KKS activity may be reduced in patients with this disease.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015002400457&lng=en&tlng=enCalicreína Tecidual HumanaCalicreína TecidualSistema Calicreína-CininaDoença Arterial Coronariana |
| spellingShingle | Estêvão Lanna Figueiredo Carolina Antunes Magalhães Karlyse Claudino Belli Ari Mandil José Carlos Faria Garcia Rosanã Aparecida Araújo Amintas Fabiano de Souza Figueiredo Lucia Campos Pellanda Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease Arquivos Brasileiros de Cardiologia Calicreína Tecidual Humana Calicreína Tecidual Sistema Calicreína-Cinina Doença Arterial Coronariana |
| title | Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease |
| title_full | Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease |
| title_fullStr | Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease |
| title_full_unstemmed | Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease |
| title_short | Human Tissue Kallikrein Activity in Angiographically Documented Chronic Stable Coronary Artery Disease |
| title_sort | human tissue kallikrein activity in angiographically documented chronic stable coronary artery disease |
| topic | Calicreína Tecidual Humana Calicreína Tecidual Sistema Calicreína-Cinina Doença Arterial Coronariana |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2015002400457&lng=en&tlng=en |
| work_keys_str_mv | AT estevaolannafigueiredo humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT carolinaantunesmagalhaes humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT karlyseclaudinobelli humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT arimandil humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT josecarlosfariagarcia humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT rosanaaparecidaaraujo humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT amintasfabianodesouzafigueiredo humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease AT luciacampospellanda humantissuekallikreinactivityinangiographicallydocumentedchronicstablecoronaryarterydisease |