Melanoma LAMP-2C Modulates Tumor Growth and Autophagy
Autophagy plays critical but diverse roles in cellular quality control and homeostasis potentially checking tumor development by removing mutated or damaged macromolecules, while conversely fostering tumor survival by supplying essential nutrients during cancer progression. This report documents a n...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-08-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fcell.2018.00101/full |
| _version_ | 1828421295789834240 |
|---|---|
| author | Liliana Pérez Anthony L. Sinn George E. Sandusky Karen E. Pollok Karen E. Pollok Karen E. Pollok Janice S. Blum |
| author_facet | Liliana Pérez Anthony L. Sinn George E. Sandusky Karen E. Pollok Karen E. Pollok Karen E. Pollok Janice S. Blum |
| author_sort | Liliana Pérez |
| collection | DOAJ |
| description | Autophagy plays critical but diverse roles in cellular quality control and homeostasis potentially checking tumor development by removing mutated or damaged macromolecules, while conversely fostering tumor survival by supplying essential nutrients during cancer progression. This report documents a novel inhibitory role for a lysosome-associated membrane protein, LAMP-2C in modulating autophagy and melanoma cell growth in vitro and in vivo. Solid tumors such as melanomas encounter a variety of stresses in vivo including inflammatory cytokines produced by infiltrating lymphocytes directed at limiting tumor growth and spread. Here, we report that in response to the anti-tumor, pro-inflammatory cytokine interferon-gamma, melanoma cell expression of LAMP2C mRNA significantly increased. These results prompted an investigation of whether increased melanoma cell expression of LAMP-2C might represent a mechanism to control or limit human melanoma growth and survival. In this study, enhanced expression of human LAMP-2C in melanoma cells perturbed macroautophagy and chaperone-mediated autophagy in several human melanoma lines. In vitro analysis showed increasing LAMP-2C expression in a melanoma cell line, triggered reduced cellular LAMP-2A and LAMP-2B protein expression. Melanoma cells with enhanced LAMP-2C expression displayed increased cell cycle arrest, increased expression of the cell cycle regulators Chk1 and p21, and greater apoptosis and necrosis in several cell lines tested. The increased abundance of Chk1 protein in melanoma cells with increased LAMP-2C expression was not due to higher CHEK1 mRNA levels, but rather an increase in Chk1 protein abundance including Chk1 molecules phosphorylated at Ser345. Human melanoma cell xenografts with increased LAMP-2C expression, displayed reduced growth in immune compromised murine hosts. Melanomas with high LAMP-2C expression showed increased necrosis and reduced cell density upon histological analysis. These results reveal a novel role for LAMP-2C in negatively regulating melanoma growth and survival. |
| first_indexed | 2024-12-10T15:26:45Z |
| format | Article |
| id | doaj.art-884441775bd94b2c9b45e56f3c054efa |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-10T15:26:45Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-884441775bd94b2c9b45e56f3c054efa2022-12-22T01:43:30ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2018-08-01610.3389/fcell.2018.00101401099Melanoma LAMP-2C Modulates Tumor Growth and AutophagyLiliana Pérez0Anthony L. Sinn1George E. Sandusky2Karen E. Pollok3Karen E. Pollok4Karen E. Pollok5Janice S. Blum6Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United StatesIn Vivo Therapeutics Core, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pathology, Indiana University School of Medicine, Indianapolis, IN, United StatesIn Vivo Therapeutics Core, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, United StatesDepartment of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, United StatesAutophagy plays critical but diverse roles in cellular quality control and homeostasis potentially checking tumor development by removing mutated or damaged macromolecules, while conversely fostering tumor survival by supplying essential nutrients during cancer progression. This report documents a novel inhibitory role for a lysosome-associated membrane protein, LAMP-2C in modulating autophagy and melanoma cell growth in vitro and in vivo. Solid tumors such as melanomas encounter a variety of stresses in vivo including inflammatory cytokines produced by infiltrating lymphocytes directed at limiting tumor growth and spread. Here, we report that in response to the anti-tumor, pro-inflammatory cytokine interferon-gamma, melanoma cell expression of LAMP2C mRNA significantly increased. These results prompted an investigation of whether increased melanoma cell expression of LAMP-2C might represent a mechanism to control or limit human melanoma growth and survival. In this study, enhanced expression of human LAMP-2C in melanoma cells perturbed macroautophagy and chaperone-mediated autophagy in several human melanoma lines. In vitro analysis showed increasing LAMP-2C expression in a melanoma cell line, triggered reduced cellular LAMP-2A and LAMP-2B protein expression. Melanoma cells with enhanced LAMP-2C expression displayed increased cell cycle arrest, increased expression of the cell cycle regulators Chk1 and p21, and greater apoptosis and necrosis in several cell lines tested. The increased abundance of Chk1 protein in melanoma cells with increased LAMP-2C expression was not due to higher CHEK1 mRNA levels, but rather an increase in Chk1 protein abundance including Chk1 molecules phosphorylated at Ser345. Human melanoma cell xenografts with increased LAMP-2C expression, displayed reduced growth in immune compromised murine hosts. Melanomas with high LAMP-2C expression showed increased necrosis and reduced cell density upon histological analysis. These results reveal a novel role for LAMP-2C in negatively regulating melanoma growth and survival.https://www.frontiersin.org/article/10.3389/fcell.2018.00101/fullLAMP-2LAMP-2Cmacroautophagychaperone-mediated autophagymelanomatumor |
| spellingShingle | Liliana Pérez Anthony L. Sinn George E. Sandusky Karen E. Pollok Karen E. Pollok Karen E. Pollok Janice S. Blum Melanoma LAMP-2C Modulates Tumor Growth and Autophagy Frontiers in Cell and Developmental Biology LAMP-2 LAMP-2C macroautophagy chaperone-mediated autophagy melanoma tumor |
| title | Melanoma LAMP-2C Modulates Tumor Growth and Autophagy |
| title_full | Melanoma LAMP-2C Modulates Tumor Growth and Autophagy |
| title_fullStr | Melanoma LAMP-2C Modulates Tumor Growth and Autophagy |
| title_full_unstemmed | Melanoma LAMP-2C Modulates Tumor Growth and Autophagy |
| title_short | Melanoma LAMP-2C Modulates Tumor Growth and Autophagy |
| title_sort | melanoma lamp 2c modulates tumor growth and autophagy |
| topic | LAMP-2 LAMP-2C macroautophagy chaperone-mediated autophagy melanoma tumor |
| url | https://www.frontiersin.org/article/10.3389/fcell.2018.00101/full |
| work_keys_str_mv | AT lilianaperez melanomalamp2cmodulatestumorgrowthandautophagy AT anthonylsinn melanomalamp2cmodulatestumorgrowthandautophagy AT georgeesandusky melanomalamp2cmodulatestumorgrowthandautophagy AT karenepollok melanomalamp2cmodulatestumorgrowthandautophagy AT karenepollok melanomalamp2cmodulatestumorgrowthandautophagy AT karenepollok melanomalamp2cmodulatestumorgrowthandautophagy AT janicesblum melanomalamp2cmodulatestumorgrowthandautophagy |