Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy
One of the plant that has antibacterial activity is chili leaf (Capsicum frutescens L.). The purpose of this study was to determine the antibacterial activity of ethyl acetate fraction of chili leaf against Streptococcus pyogenes bacteria and conduct bioautography to see compounds that have antibac...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Indonesian |
| Published: |
Universitas Muhammadiyah Magelang
2019-12-01
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| Series: | Jurnal Farmasi Sains dan Praktis |
| Subjects: | |
| Online Access: | http://journal.ummgl.ac.id/index.php/pharmacy/article/view/3002 |
| _version_ | 1818116864000131072 |
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| author | Ade Irawan Nurkhasanah - Nanik Sulistyani |
| author_facet | Ade Irawan Nurkhasanah - Nanik Sulistyani |
| author_sort | Ade Irawan |
| collection | DOAJ |
| description | One of the plant that has antibacterial activity is chili leaf (Capsicum frutescens L.). The purpose of this study was to determine the antibacterial activity of ethyl acetate fraction of chili leaf against Streptococcus pyogenes bacteria and conduct bioautography to see compounds that have antibacterial activity. In this study an antibacterial test for ethyl acetate fraction of chili leaf with a concentration of 5%, 10%, 20%, 40%, 80% to Streptococcus pyogenes. From the results of the inhibition zone test 5% ethyl acetate fraction produces an average inhibition zone of 6.3 mm, 10% average inhibition zone 10.3 mm, 20% average inhibition zone of 14 mm, 40% average inhibition zone 18 mm and 80% have 22.7 an average inhibition zone. In the silent phase bioautographic was used test silica gel GF 254 and for the mobile phase was used chloroform : ethyl acetate (3: 7), indicating the presence of inhibition zones on the media which had been inoculated by Streptococcus pyogenes bacteria, namely at RF 0.88. From the research that has been done, the results show that the ethyl acetate fraction of chili leaf has inhibitory zone against Streptococcus pyogenes bacteria and the spotting of compounds which have inhibitory zone has an RF of 0.88. |
| first_indexed | 2024-12-11T04:29:17Z |
| format | Article |
| id | doaj.art-884581a79d3745cbafc4304122b4f6f2 |
| institution | Directory Open Access Journal |
| issn | 2549-9068 2579-4558 |
| language | Indonesian |
| last_indexed | 2024-12-11T04:29:17Z |
| publishDate | 2019-12-01 |
| publisher | Universitas Muhammadiyah Magelang |
| record_format | Article |
| series | Jurnal Farmasi Sains dan Praktis |
| spelling | doaj.art-884581a79d3745cbafc4304122b4f6f22022-12-22T01:20:54ZindUniversitas Muhammadiyah MagelangJurnal Farmasi Sains dan Praktis2549-90682579-45582019-12-015210.31603/pharmacy.v5i2.3002Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and BioautografyAde Irawan0Nurkhasanah -Nanik SulistyaniSTIKES MUHAMMADIYAH CIREBONOne of the plant that has antibacterial activity is chili leaf (Capsicum frutescens L.). The purpose of this study was to determine the antibacterial activity of ethyl acetate fraction of chili leaf against Streptococcus pyogenes bacteria and conduct bioautography to see compounds that have antibacterial activity. In this study an antibacterial test for ethyl acetate fraction of chili leaf with a concentration of 5%, 10%, 20%, 40%, 80% to Streptococcus pyogenes. From the results of the inhibition zone test 5% ethyl acetate fraction produces an average inhibition zone of 6.3 mm, 10% average inhibition zone 10.3 mm, 20% average inhibition zone of 14 mm, 40% average inhibition zone 18 mm and 80% have 22.7 an average inhibition zone. In the silent phase bioautographic was used test silica gel GF 254 and for the mobile phase was used chloroform : ethyl acetate (3: 7), indicating the presence of inhibition zones on the media which had been inoculated by Streptococcus pyogenes bacteria, namely at RF 0.88. From the research that has been done, the results show that the ethyl acetate fraction of chili leaf has inhibitory zone against Streptococcus pyogenes bacteria and the spotting of compounds which have inhibitory zone has an RF of 0.88.http://journal.ummgl.ac.id/index.php/pharmacy/article/view/3002Antibacterial, Inhibitory Zone, Chili Leaf |
| spellingShingle | Ade Irawan Nurkhasanah - Nanik Sulistyani Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy Jurnal Farmasi Sains dan Praktis Antibacterial, Inhibitory Zone, Chili Leaf |
| title | Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy |
| title_full | Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy |
| title_fullStr | Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy |
| title_full_unstemmed | Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy |
| title_short | Antibacterial Activity of Etil Acetate Fraction of Chili Leaf Against Streptococcus Pyogenes and Bioautografy |
| title_sort | antibacterial activity of etil acetate fraction of chili leaf against streptococcus pyogenes and bioautografy |
| topic | Antibacterial, Inhibitory Zone, Chili Leaf |
| url | http://journal.ummgl.ac.id/index.php/pharmacy/article/view/3002 |
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