Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets.
Does diet-induced obesity persist after an obesigenic diet is removed? We investigated this question by providing male C57BL/6 mice with free access to two different obesigenic diets followed by a switch to chow to determine if obesity was reversible.Male C57BL/6 mice were randomly assigned to five...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2670508?pdf=render |
| _version_ | 1818765640267202560 |
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| author | Juen Guo William Jou Oksana Gavrilova Kevin D Hall |
| author_facet | Juen Guo William Jou Oksana Gavrilova Kevin D Hall |
| author_sort | Juen Guo |
| collection | DOAJ |
| description | Does diet-induced obesity persist after an obesigenic diet is removed? We investigated this question by providing male C57BL/6 mice with free access to two different obesigenic diets followed by a switch to chow to determine if obesity was reversible.Male C57BL/6 mice were randomly assigned to five weight-matched groups: 1) C group that continuously received a chow diet; 2) HF group on a 60% high fat diet; 3) EN group on the high fat diet plus liquid Ensure; 4) HF-C group switched from high fat to chow after 7 weeks; 5) EN-C group switched from high fat plus Ensure to chow after 7 weeks. All food intake was ad libitum. Body weight was increased after 7 weeks on both obesigenic diets (44.6+/-0.65, 39.8+/-0.63, and 28.6+/-0.63 g for EN, HF, and C groups, respectively) and resulted in elevated concentrations of serum insulin, glucose, and leptin and lower serum triglycerides. Development of obesity in HF and EN mice was caused by increased energy intake and a relative decrease of average energy output along with decreased ambulatory activity. After the switch to chow, the HF-C and EN-C groups lost weight but subsequently maintained a state of persistent obesity in comparison to the C group (34.8+/-1.2, 34.1+/-1.2 vs. 30.8+/-0.8 g respectively; P<0.05) with a 40-50% increase of body fat. All serum hormones and metabolites returned to control levels with the exception of a trend for increased leptin. The HF-C and EN-C groups had an average energy output in line with the C group and the persistent obesity was maintained despite a non-significant increase of energy intake of less than 1 kcal/d at the end of the study.Our results illustrate the importance of considering the history of energy imbalance in determining body weight and that a persistent elevation of body weight after removal of obesigenic diets can result from very small increases of energy intake. |
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| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-18T08:21:18Z |
| publishDate | 2009-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-8849cdb2e20542158c3bf5250456b4222022-12-21T21:14:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-01-0144e537010.1371/journal.pone.0005370Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets.Juen GuoWilliam JouOksana GavrilovaKevin D HallDoes diet-induced obesity persist after an obesigenic diet is removed? We investigated this question by providing male C57BL/6 mice with free access to two different obesigenic diets followed by a switch to chow to determine if obesity was reversible.Male C57BL/6 mice were randomly assigned to five weight-matched groups: 1) C group that continuously received a chow diet; 2) HF group on a 60% high fat diet; 3) EN group on the high fat diet plus liquid Ensure; 4) HF-C group switched from high fat to chow after 7 weeks; 5) EN-C group switched from high fat plus Ensure to chow after 7 weeks. All food intake was ad libitum. Body weight was increased after 7 weeks on both obesigenic diets (44.6+/-0.65, 39.8+/-0.63, and 28.6+/-0.63 g for EN, HF, and C groups, respectively) and resulted in elevated concentrations of serum insulin, glucose, and leptin and lower serum triglycerides. Development of obesity in HF and EN mice was caused by increased energy intake and a relative decrease of average energy output along with decreased ambulatory activity. After the switch to chow, the HF-C and EN-C groups lost weight but subsequently maintained a state of persistent obesity in comparison to the C group (34.8+/-1.2, 34.1+/-1.2 vs. 30.8+/-0.8 g respectively; P<0.05) with a 40-50% increase of body fat. All serum hormones and metabolites returned to control levels with the exception of a trend for increased leptin. The HF-C and EN-C groups had an average energy output in line with the C group and the persistent obesity was maintained despite a non-significant increase of energy intake of less than 1 kcal/d at the end of the study.Our results illustrate the importance of considering the history of energy imbalance in determining body weight and that a persistent elevation of body weight after removal of obesigenic diets can result from very small increases of energy intake.http://europepmc.org/articles/PMC2670508?pdf=render |
| spellingShingle | Juen Guo William Jou Oksana Gavrilova Kevin D Hall Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. PLoS ONE |
| title | Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. |
| title_full | Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. |
| title_fullStr | Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. |
| title_full_unstemmed | Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. |
| title_short | Persistent diet-induced obesity in male C57BL/6 mice resulting from temporary obesigenic diets. |
| title_sort | persistent diet induced obesity in male c57bl 6 mice resulting from temporary obesigenic diets |
| url | http://europepmc.org/articles/PMC2670508?pdf=render |
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