Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing
Abstract The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-01-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-020-01158-5 |
| _version_ | 1818562720283230208 |
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| author | Zongfu Hu Deying Ma Huaxin Niu Jie Chang Jianhua Yu Qing Tong Shuguo Li |
| author_facet | Zongfu Hu Deying Ma Huaxin Niu Jie Chang Jianhua Yu Qing Tong Shuguo Li |
| author_sort | Zongfu Hu |
| collection | DOAJ |
| description | Abstract The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP). |
| first_indexed | 2024-12-14T01:07:28Z |
| format | Article |
| id | doaj.art-884b31d1fb404c75908ae140688b869f |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-14T01:07:28Z |
| publishDate | 2021-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-884b31d1fb404c75908ae140688b869f2022-12-21T23:22:55ZengSpringerOpenAMB Express2191-08552021-01-0111111110.1186/s13568-020-01158-5Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencingZongfu Hu0Deying Ma1Huaxin Niu2Jie Chang3Jianhua Yu4Qing Tong5Shuguo Li6College of Animal Science and Technology, Northeast Agricultural UniversityCollege of Animal Science and Technology, Northeast Agricultural UniversityCollege of Animal Science and Technology, Inner Mongolia University for NationalitiesCollege of Animal Science and Technology, Inner Mongolia University for NationalitiesCollege of Animal Science and Technology, Northeast Agricultural UniversityCollege of Animal Science and Technology, Northeast Agricultural UniversityCollege of Animal Science and Technology, Inner Mongolia University for NationalitiesAbstract The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).https://doi.org/10.1186/s13568-020-01158-5Illumina sequencingBacterial communityCellulaseɑ-GalactosidaseL. plantarumAlfalfa silage |
| spellingShingle | Zongfu Hu Deying Ma Huaxin Niu Jie Chang Jianhua Yu Qing Tong Shuguo Li Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing AMB Express Illumina sequencing Bacterial community Cellulase ɑ-Galactosidase L. plantarum Alfalfa silage |
| title | Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing |
| title_full | Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing |
| title_fullStr | Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing |
| title_full_unstemmed | Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing |
| title_short | Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing |
| title_sort | enzyme additives influence bacterial communities of medicago sativa silage as determined by illumina sequencing |
| topic | Illumina sequencing Bacterial community Cellulase ɑ-Galactosidase L. plantarum Alfalfa silage |
| url | https://doi.org/10.1186/s13568-020-01158-5 |
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