Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea

In this study, we investigated the reproductive pattern of the commercially and ecologically important species, <i>Ostrea denelamellosa</i>, to inform stock management strategies in South Korea. Prior to the reproduction experiment, the complete mitochondrial (mt) genome of the flat oyst...

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Main Authors: Jeonghoon Han, Han-Jun Kim, Sung-Yong Oh, Young-Ung Choi
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Journal of Marine Science and Engineering
Subjects:
Online Access:https://www.mdpi.com/2077-1312/10/9/1326
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author Jeonghoon Han
Han-Jun Kim
Sung-Yong Oh
Young-Ung Choi
author_facet Jeonghoon Han
Han-Jun Kim
Sung-Yong Oh
Young-Ung Choi
author_sort Jeonghoon Han
collection DOAJ
description In this study, we investigated the reproductive pattern of the commercially and ecologically important species, <i>Ostrea denelamellosa</i>, to inform stock management strategies in South Korea. Prior to the reproduction experiment, the complete mitochondrial (mt) genome of the flat oyster, <i>Ostrea denselamellosa</i>, was analyzed using next-generation sequencing technology. Then, to determine the reproductive pattern of <i>Ostrea denselamellosa</i>, we investigated monthly changes in the gametogenesis, reproductive cycle, and sex ratio from January to October 2021 in females. The total length of the mt genome sequence of <i>O. denselamellosa</i> was 16,225 bp and contained 37 genes (13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes). Molecular phylogenetic comparison with 20 known species of Pteriomorphia showed that <i>O. denselamellosa</i> belongs to the family Ostreidae. In addition, <i>O. denselamellosa</i> clustered together with the <i>O. denselamellosa</i> Chinese strain, with a bootstrap value of 100%. Histological analysis indicated a discrepancy in gamete development of <i>O. denselamellosa</i> with synchronous maturation of oocytes and asynchronous development of spermatozoa in gonads. The spawning activity occurred between May and September with a temperature range gap of 6.5 °C. The spawning activity occurred from May when the temperature reached 16.7 °C until September when the temperature dropped below 23.2 °C. Furthermore, sex ratio bias was observed. This is the first study to report the complete mt genome sequence and examine the reproductive pattern in native <i>O. denselamellosa</i> in South Korea. Overall, these findings will help enhance the knowledge for the management and sustainable fishery of endangered oyster species including <i>O. denselamellosa</i> in the South Sea of Korea.
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spelling doaj.art-884b49bf12f641088af9e8cccd8642552023-11-23T17:08:32ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-09-01109132610.3390/jmse10091326Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South KoreaJeonghoon Han0Han-Jun Kim1Sung-Yong Oh2Young-Ung Choi3Marine Bio-Resources Research Unit, Korea Institute of Ocean Science & Technology (KIOST), Busan 49111, KoreaMarine Bio-Resources Research Unit, Korea Institute of Ocean Science & Technology (KIOST), Busan 49111, KoreaMarine Bio-Resources Research Unit, Korea Institute of Ocean Science & Technology (KIOST), Busan 49111, KoreaMarine Bio-Resources Research Unit, Korea Institute of Ocean Science & Technology (KIOST), Busan 49111, KoreaIn this study, we investigated the reproductive pattern of the commercially and ecologically important species, <i>Ostrea denelamellosa</i>, to inform stock management strategies in South Korea. Prior to the reproduction experiment, the complete mitochondrial (mt) genome of the flat oyster, <i>Ostrea denselamellosa</i>, was analyzed using next-generation sequencing technology. Then, to determine the reproductive pattern of <i>Ostrea denselamellosa</i>, we investigated monthly changes in the gametogenesis, reproductive cycle, and sex ratio from January to October 2021 in females. The total length of the mt genome sequence of <i>O. denselamellosa</i> was 16,225 bp and contained 37 genes (13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes). Molecular phylogenetic comparison with 20 known species of Pteriomorphia showed that <i>O. denselamellosa</i> belongs to the family Ostreidae. In addition, <i>O. denselamellosa</i> clustered together with the <i>O. denselamellosa</i> Chinese strain, with a bootstrap value of 100%. Histological analysis indicated a discrepancy in gamete development of <i>O. denselamellosa</i> with synchronous maturation of oocytes and asynchronous development of spermatozoa in gonads. The spawning activity occurred between May and September with a temperature range gap of 6.5 °C. The spawning activity occurred from May when the temperature reached 16.7 °C until September when the temperature dropped below 23.2 °C. Furthermore, sex ratio bias was observed. This is the first study to report the complete mt genome sequence and examine the reproductive pattern in native <i>O. denselamellosa</i> in South Korea. Overall, these findings will help enhance the knowledge for the management and sustainable fishery of endangered oyster species including <i>O. denselamellosa</i> in the South Sea of Korea.https://www.mdpi.com/2077-1312/10/9/1326bivalvemarine resourcesphylogenyenvironmental factorsgametogenesisgonad development
spellingShingle Jeonghoon Han
Han-Jun Kim
Sung-Yong Oh
Young-Ung Choi
Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
Journal of Marine Science and Engineering
bivalve
marine resources
phylogeny
environmental factors
gametogenesis
gonad development
title Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
title_full Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
title_fullStr Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
title_full_unstemmed Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
title_short Reproductive Characteristics of the Flat Oyster <i>Ostrea denselamellosa</i> (Bivalvia, Ostreidae) Found on the Southern Coast of South Korea
title_sort reproductive characteristics of the flat oyster i ostrea denselamellosa i bivalvia ostreidae found on the southern coast of south korea
topic bivalve
marine resources
phylogeny
environmental factors
gametogenesis
gonad development
url https://www.mdpi.com/2077-1312/10/9/1326
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AT hanjunkim reproductivecharacteristicsoftheflatoysteriostreadenselamellosaibivalviaostreidaefoundonthesoutherncoastofsouthkorea
AT sungyongoh reproductivecharacteristicsoftheflatoysteriostreadenselamellosaibivalviaostreidaefoundonthesoutherncoastofsouthkorea
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