Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue
A genetic background has been implicated in the development of prostate cancer. Protein microarrays have enabled the identification of proteins, some of which associated with apoptosis, that may play a role in the development of such a tumor. Inhibition of apoptosis is a co-factor that contributes t...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2013-04-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2155 |
| _version_ | 1818186864946839552 |
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| author | M. Salemi A. Galia F. Fraggetta C. La Corte P. Pepe S. La Vignera G. Improta P. Bosco A.E. Calogero |
| author_facet | M. Salemi A. Galia F. Fraggetta C. La Corte P. Pepe S. La Vignera G. Improta P. Bosco A.E. Calogero |
| author_sort | M. Salemi |
| collection | DOAJ |
| description | A genetic background has been implicated in the development of prostate cancer. Protein microarrays have enabled the identification of proteins, some of which associated with apoptosis, that may play a role in the development of such a tumor. Inhibition of apoptosis is a co-factor that contributes to the onset and progression of prostate cancer, though the molecular mechanisms are not entirely understood. Poly (ADP-ribose) polymerase 1 (<em>PARP-1</em>) gene is required for translocation of the apoptosis-inducing factor (AIF) from the mitochondria to the nucleus. Hence, it is involved in programmed cell death. Different <em>PARP-1</em> gene expression has been observed in various tumors such as glioblastoma, lung, ovarian, endometrial, and skin cancers. We evaluated the expression of PARP-1 protein in prostatic cancer and normal prostate tissues by immunohistochemistry in 40 men with prostate cancer and in 37 normal men. Positive nuclear PARP-1 staining was found in all samples (normal prostate and prostate cancer tissues). No cytoplasmic staining was observed in any sample. PARP-1-positive cells resulted significantly higher in patients with prostate carcinoma compared with controls (P<0.001).<em> PARP-1</em> over-expression in prostate cancer tissue compared with normal prostate suggests a greater activity of<em> PARP-1</em> in these tumors. These findings suggest that PARP-1 expression in prostate cancer is an attempt to trigger apoptosis in this type of tumor similarly to what reported in other cancers. |
| first_indexed | 2024-12-11T23:01:55Z |
| format | Article |
| id | doaj.art-884d3b7934724a9f8cbbb09b2b741f9a |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-11T23:01:55Z |
| publishDate | 2013-04-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-884d3b7934724a9f8cbbb09b2b741f9a2022-12-22T00:47:03ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062013-04-01572e13e1310.4081/ejh.2013.e131330Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissueM. Salemi0A. Galia1F. Fraggetta2C. La Corte3P. Pepe4S. La Vignera5G. Improta6P. Bosco7A.E. Calogero8University of CataniaCannizzaro Hospital, CataniaCannizzaro Hospital, CataniaCannizzaro Hospital, CataniaCannizzaro Hospital, CataniaUniversity of CataniaIRCCS-CROB Hospital, Rionero in VultureOasi Institute for Research on Mental Retardation and Brain Aging, Troina, EnnaUniversity of CataniaA genetic background has been implicated in the development of prostate cancer. Protein microarrays have enabled the identification of proteins, some of which associated with apoptosis, that may play a role in the development of such a tumor. Inhibition of apoptosis is a co-factor that contributes to the onset and progression of prostate cancer, though the molecular mechanisms are not entirely understood. Poly (ADP-ribose) polymerase 1 (<em>PARP-1</em>) gene is required for translocation of the apoptosis-inducing factor (AIF) from the mitochondria to the nucleus. Hence, it is involved in programmed cell death. Different <em>PARP-1</em> gene expression has been observed in various tumors such as glioblastoma, lung, ovarian, endometrial, and skin cancers. We evaluated the expression of PARP-1 protein in prostatic cancer and normal prostate tissues by immunohistochemistry in 40 men with prostate cancer and in 37 normal men. Positive nuclear PARP-1 staining was found in all samples (normal prostate and prostate cancer tissues). No cytoplasmic staining was observed in any sample. PARP-1-positive cells resulted significantly higher in patients with prostate carcinoma compared with controls (P<0.001).<em> PARP-1</em> over-expression in prostate cancer tissue compared with normal prostate suggests a greater activity of<em> PARP-1</em> in these tumors. These findings suggest that PARP-1 expression in prostate cancer is an attempt to trigger apoptosis in this type of tumor similarly to what reported in other cancers.http://www.ejh.it/index.php/ejh/article/view/2155prostate cancer, PARP-1 geneprotein expressionimmunoistochemistry |
| spellingShingle | M. Salemi A. Galia F. Fraggetta C. La Corte P. Pepe S. La Vignera G. Improta P. Bosco A.E. Calogero Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue European Journal of Histochemistry prostate cancer, PARP-1 gene protein expression immunoistochemistry |
| title | Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| title_full | Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| title_fullStr | Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| title_full_unstemmed | Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| title_short | Poly (ADP-ribose) polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| title_sort | poly adp ribose polymerase 1 protein expression in normal and neoplastic prostatic tissue |
| topic | prostate cancer, PARP-1 gene protein expression immunoistochemistry |
| url | http://www.ejh.it/index.php/ejh/article/view/2155 |
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