Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs
<p>Abstract</p> <p>Background</p> <p>Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate wh...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://respiratory-research.com/content/13/1/103 |
| _version_ | 1818835479596892160 |
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| author | Shimodaira Kayoko Okubo Yoichiro Ochiai Eri Nakayama Haruo Katano Harutaka Wakayama Megumi Shinozaki Minoru Ishiwatari Takao Sasai Daisuke Tochigi Naobumi Nemoto Tetsuo Saji Tsutomu Kamei Katsuhiko Shibuya Kazutoshi |
| author_facet | Shimodaira Kayoko Okubo Yoichiro Ochiai Eri Nakayama Haruo Katano Harutaka Wakayama Megumi Shinozaki Minoru Ishiwatari Takao Sasai Daisuke Tochigi Naobumi Nemoto Tetsuo Saji Tsutomu Kamei Katsuhiko Shibuya Kazutoshi |
| author_sort | Shimodaira Kayoko |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate what essentially contributes to the onset and progression of IPAH, we investigated factors playing an important role in IPAH by searching discrepant or controversial expression patterns between our murine model and those previously published for human IPAH. We employed the mouse model, which induced muscularization of pulmonary artery leading to hypertension by repeated intratracheal injection of <it>Stachybotrys chartarum</it>, a member of nonpathogenic and ubiquitous fungus in our envelopment.</p> <p>Methods</p> <p>Microarray assays with ontology and pathway analyses were performed with the lungs of mice. A comparison was made of the expression patterns of biological pathways between our model and those published for IPAH.</p> <p>Results</p> <p>Some pathways in our model showed the same expression patterns in IPAH, which included bone morphogenetic protein (BMP) signaling with down-regulation of BMP receptor type 2, activin-like kinase type 1, and endoglin. On the other hand, both Wnt/planar cell polarity (PCP) signaling and its downstream Rho/ROCK signaling were found alone to be activated in IPAH and not in our model.</p> <p>Conclusions</p> <p>Activation of Wnt/PCP signaling, in upstream positions of the pathway, found alone in lungs from end stage IPAH may play essential roles in the pathogenesis of the disease.</p> |
| first_indexed | 2024-12-19T02:51:22Z |
| format | Article |
| id | doaj.art-884db002e0494fc8b431f30dbfb599a9 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-19T02:51:22Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-884db002e0494fc8b431f30dbfb599a92022-12-21T20:38:36ZengBMCRespiratory Research1465-99212012-11-0113110310.1186/1465-9921-13-103Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungsShimodaira KayokoOkubo YoichiroOchiai EriNakayama HaruoKatano HarutakaWakayama MegumiShinozaki MinoruIshiwatari TakaoSasai DaisukeTochigi NaobumiNemoto TetsuoSaji TsutomuKamei KatsuhikoShibuya Kazutoshi<p>Abstract</p> <p>Background</p> <p>Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate what essentially contributes to the onset and progression of IPAH, we investigated factors playing an important role in IPAH by searching discrepant or controversial expression patterns between our murine model and those previously published for human IPAH. We employed the mouse model, which induced muscularization of pulmonary artery leading to hypertension by repeated intratracheal injection of <it>Stachybotrys chartarum</it>, a member of nonpathogenic and ubiquitous fungus in our envelopment.</p> <p>Methods</p> <p>Microarray assays with ontology and pathway analyses were performed with the lungs of mice. A comparison was made of the expression patterns of biological pathways between our model and those published for IPAH.</p> <p>Results</p> <p>Some pathways in our model showed the same expression patterns in IPAH, which included bone morphogenetic protein (BMP) signaling with down-regulation of BMP receptor type 2, activin-like kinase type 1, and endoglin. On the other hand, both Wnt/planar cell polarity (PCP) signaling and its downstream Rho/ROCK signaling were found alone to be activated in IPAH and not in our model.</p> <p>Conclusions</p> <p>Activation of Wnt/PCP signaling, in upstream positions of the pathway, found alone in lungs from end stage IPAH may play essential roles in the pathogenesis of the disease.</p>http://respiratory-research.com/content/13/1/103Pulmonary Vascular Remodeling<it>Stachybotrys chartarum</it>BMP signalingBMPR2PCP pathway |
| spellingShingle | Shimodaira Kayoko Okubo Yoichiro Ochiai Eri Nakayama Haruo Katano Harutaka Wakayama Megumi Shinozaki Minoru Ishiwatari Takao Sasai Daisuke Tochigi Naobumi Nemoto Tetsuo Saji Tsutomu Kamei Katsuhiko Shibuya Kazutoshi Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs Respiratory Research Pulmonary Vascular Remodeling <it>Stachybotrys chartarum</it> BMP signaling BMPR2 PCP pathway |
| title | Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs |
| title_full | Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs |
| title_fullStr | Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs |
| title_full_unstemmed | Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs |
| title_short | Gene expression analysis of a murine model with pulmonary vascular remodeling compared to end-stage IPAH lungs |
| title_sort | gene expression analysis of a murine model with pulmonary vascular remodeling compared to end stage ipah lungs |
| topic | Pulmonary Vascular Remodeling <it>Stachybotrys chartarum</it> BMP signaling BMPR2 PCP pathway |
| url | http://respiratory-research.com/content/13/1/103 |
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