Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants
Abstract Background To explore the community composition and diversity of the endophytic fungi in Taxillus chinensis, samples of the parasites growing on seven different hosts, Morus alba, Prunus salicina, Phellodendron chinense, Bauhinia purpurea, Dalbergia odorifera, Diospyros kaki and Dimocarpus...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-023-02878-x |
| _version_ | 1827948278088466432 |
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| author | Li-sha Song Juan Huo Lingyun Wan Limei Pan Ni Jiang Jine Fu Shugen Wei Lili He |
| author_facet | Li-sha Song Juan Huo Lingyun Wan Limei Pan Ni Jiang Jine Fu Shugen Wei Lili He |
| author_sort | Li-sha Song |
| collection | DOAJ |
| description | Abstract Background To explore the community composition and diversity of the endophytic fungi in Taxillus chinensis, samples of the parasites growing on seven different hosts, Morus alba, Prunus salicina, Phellodendron chinense, Bauhinia purpurea, Dalbergia odorifera, Diospyros kaki and Dimocarpus longan, were isolated. The strains were identified by their morphological characteristics and their internal transcribed spacer (ITS) sequences. Results 150 different endophytic fungi were isolated from the haustorial roots of the seven hosts with a total isolation rate of 61.24%. These endophytic fungi were found to belong to 1 phylum, 2 classes, 7 orders, 9 families, 11 genera and 8 species. Among of them, Pestalotiopsis, Neopestalotiopsis and Diaporthe were the dominant genera, accounting for 26.67, 17.33 and 31.33% of the total number of strains, respectively. Diversity and similarity analyses showed that the endophytic fungi isolated from D. longan (H’=1.60) had the highest diversity index. The highest richness indexes were found in M. alba and D. odorifera (both 2.23). The evenness index of D. longan was the highest (0.82). The similarity coefficient of D. odorifera was the most similar to D. longan and M. alba (33.33%), while the similarity coefficient of P. chinense was the lowest (7.69%) with M. alba and D. odorifera. Nine strains showed antimicrobial activities. Among them, Pestalotiopsis sp., N. parvum and H. investiens showed significant antifungal activity against three fungal phytopathogens of medicinal plants. At the same time, the crude extracts from the metabolites of the three endophytic fungi had strong inhibitory effects on the three pathogens. Pestalotiopsis sp., N. parvum and H. investiens had the strongest inhibitory effects of S. cucurbitacearum, with inhibitory rates of 100%, 100% and 81.51%, respectively. In addition, N. parvum had a strong inhibitory effect on D. glomerata and C. cassicola, with inhibitory rates of 82.35% and 72.80%, respectively. Conclusions These results indicate that the species composition and diversity of endophytic fungi in the branches of T. chinensis were varied in the different hosts and showed good antimicrobial potential in the control of plant pathogens. |
| first_indexed | 2024-04-09T12:51:46Z |
| format | Article |
| id | doaj.art-884f4cbcc22b4ce5bed9acb546299220 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-09T12:51:46Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-884f4cbcc22b4ce5bed9acb5462992202023-05-14T11:10:21ZengBMCBMC Microbiology1471-21802023-05-0123111210.1186/s12866-023-02878-xDifferences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plantsLi-sha Song0Juan Huo1Lingyun Wan2Limei Pan3Ni Jiang4Jine Fu5Shugen Wei6Lili He7Guangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsGuangxi Botanical Garden of Medicinal PlantsAbstract Background To explore the community composition and diversity of the endophytic fungi in Taxillus chinensis, samples of the parasites growing on seven different hosts, Morus alba, Prunus salicina, Phellodendron chinense, Bauhinia purpurea, Dalbergia odorifera, Diospyros kaki and Dimocarpus longan, were isolated. The strains were identified by their morphological characteristics and their internal transcribed spacer (ITS) sequences. Results 150 different endophytic fungi were isolated from the haustorial roots of the seven hosts with a total isolation rate of 61.24%. These endophytic fungi were found to belong to 1 phylum, 2 classes, 7 orders, 9 families, 11 genera and 8 species. Among of them, Pestalotiopsis, Neopestalotiopsis and Diaporthe were the dominant genera, accounting for 26.67, 17.33 and 31.33% of the total number of strains, respectively. Diversity and similarity analyses showed that the endophytic fungi isolated from D. longan (H’=1.60) had the highest diversity index. The highest richness indexes were found in M. alba and D. odorifera (both 2.23). The evenness index of D. longan was the highest (0.82). The similarity coefficient of D. odorifera was the most similar to D. longan and M. alba (33.33%), while the similarity coefficient of P. chinense was the lowest (7.69%) with M. alba and D. odorifera. Nine strains showed antimicrobial activities. Among them, Pestalotiopsis sp., N. parvum and H. investiens showed significant antifungal activity against three fungal phytopathogens of medicinal plants. At the same time, the crude extracts from the metabolites of the three endophytic fungi had strong inhibitory effects on the three pathogens. Pestalotiopsis sp., N. parvum and H. investiens had the strongest inhibitory effects of S. cucurbitacearum, with inhibitory rates of 100%, 100% and 81.51%, respectively. In addition, N. parvum had a strong inhibitory effect on D. glomerata and C. cassicola, with inhibitory rates of 82.35% and 72.80%, respectively. Conclusions These results indicate that the species composition and diversity of endophytic fungi in the branches of T. chinensis were varied in the different hosts and showed good antimicrobial potential in the control of plant pathogens.https://doi.org/10.1186/s12866-023-02878-xPlant endophytesInhibitory activityBiodiversityPhylogenyTaxillus chinensis |
| spellingShingle | Li-sha Song Juan Huo Lingyun Wan Limei Pan Ni Jiang Jine Fu Shugen Wei Lili He Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants BMC Microbiology Plant endophytes Inhibitory activity Biodiversity Phylogeny Taxillus chinensis |
| title | Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants |
| title_full | Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants |
| title_fullStr | Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants |
| title_full_unstemmed | Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants |
| title_short | Differences and biocontrol potential of haustorial endophytic fungi from Taxillus Chinensis on different host plants |
| title_sort | differences and biocontrol potential of haustorial endophytic fungi from taxillus chinensis on different host plants |
| topic | Plant endophytes Inhibitory activity Biodiversity Phylogeny Taxillus chinensis |
| url | https://doi.org/10.1186/s12866-023-02878-x |
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