Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries
The objective was to investigate the effects of semen freezing extender supplementation with antibiotics on bacterial load of semen samples, sperm functional and morphological metrics in the collared peccary. Fresh ejaculates from 10 males were extended in Tris-egg yolk-glycerol supplemented or not...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.954921/full |
| _version_ | 1798024945905696768 |
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| author | Samara S. J. Moreira Caio S. Santos Thibério S. Castelo Luana G. P. Bezerra Érica C. G. Praxedes Tayná M. Matos João B. F. Souza-Junior Francisco M. C. Feijó Pierre Comizzoli Alexandre R. Silva |
| author_facet | Samara S. J. Moreira Caio S. Santos Thibério S. Castelo Luana G. P. Bezerra Érica C. G. Praxedes Tayná M. Matos João B. F. Souza-Junior Francisco M. C. Feijó Pierre Comizzoli Alexandre R. Silva |
| author_sort | Samara S. J. Moreira |
| collection | DOAJ |
| description | The objective was to investigate the effects of semen freezing extender supplementation with antibiotics on bacterial load of semen samples, sperm functional and morphological metrics in the collared peccary. Fresh ejaculates from 10 males were extended in Tris-egg yolk-glycerol supplemented or not (control) with gentamicin (70 μg/mL) streptomycin-penicillin (SP; 1 mg/mL−1000 IU/mL) or and cryopreserved in liquid nitrogen. Bacterial load, sperm motility patterns, morphology, membrane functionality and integrity, mitochondrial activity, chromatin integrity and sperm-binding ability were evaluated in fresh and frozen-thawed samples. Regardless of the use of antibiotics, the sole cryopreservation provoked a significant decrease (P < 0.05) in bacterial load compared to fresh samples (from average values > 1 x 106 CFU/mL to <0.4 × 106 CFU/mL). Post-thawing sperm kinetic parameters were not affected by the absence or presence of different antibiotics, except for beat cross frequency that was significantly (P < 0.05) impaired by SP supplementation compared to the group without antibiotics. After thawing, sperm morphology, membrane functionality and integrity, and mitochondrial activity were also not affected by the presence or absence of antibiotics; however, a significant decrease was observed in the group without antibiotics (P < 0.05) in comparison to fresh samples. Regarding sperm-binding ability, there were no differences among the different groups. While collared peccary semen could be efficiently cryopreserved in the absence of antibiotics in the extender, the use of both gentamicin or the streptomycin-penicillin combination is recommended as effective antibiotic supplementation for a further control of bacterial loads without affecting sperm parameters. |
| first_indexed | 2024-04-11T18:10:58Z |
| format | Article |
| id | doaj.art-88529732cd95452fbb599f83a25cdd74 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-11T18:10:58Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-88529732cd95452fbb599f83a25cdd742022-12-22T04:10:07ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-09-01910.3389/fvets.2022.954921954921Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccariesSamara S. J. Moreira0Caio S. Santos1Thibério S. Castelo2Luana G. P. Bezerra3Érica C. G. Praxedes4Tayná M. Matos5João B. F. Souza-Junior6Francisco M. C. Feijó7Pierre Comizzoli8Alexandre R. Silva9Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilLaboratory of Veterinary Microbiology, UFERSA, Mossoró, RN, BrazilSmithsonian National Zoo and Conservation Biology Institute, Washington, DC, United StatesLaboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid (UFERSA), Mossoró, RN, BrazilThe objective was to investigate the effects of semen freezing extender supplementation with antibiotics on bacterial load of semen samples, sperm functional and morphological metrics in the collared peccary. Fresh ejaculates from 10 males were extended in Tris-egg yolk-glycerol supplemented or not (control) with gentamicin (70 μg/mL) streptomycin-penicillin (SP; 1 mg/mL−1000 IU/mL) or and cryopreserved in liquid nitrogen. Bacterial load, sperm motility patterns, morphology, membrane functionality and integrity, mitochondrial activity, chromatin integrity and sperm-binding ability were evaluated in fresh and frozen-thawed samples. Regardless of the use of antibiotics, the sole cryopreservation provoked a significant decrease (P < 0.05) in bacterial load compared to fresh samples (from average values > 1 x 106 CFU/mL to <0.4 × 106 CFU/mL). Post-thawing sperm kinetic parameters were not affected by the absence or presence of different antibiotics, except for beat cross frequency that was significantly (P < 0.05) impaired by SP supplementation compared to the group without antibiotics. After thawing, sperm morphology, membrane functionality and integrity, and mitochondrial activity were also not affected by the presence or absence of antibiotics; however, a significant decrease was observed in the group without antibiotics (P < 0.05) in comparison to fresh samples. Regarding sperm-binding ability, there were no differences among the different groups. While collared peccary semen could be efficiently cryopreserved in the absence of antibiotics in the extender, the use of both gentamicin or the streptomycin-penicillin combination is recommended as effective antibiotic supplementation for a further control of bacterial loads without affecting sperm parameters.https://www.frontiersin.org/articles/10.3389/fvets.2022.954921/fullwildlifebiobankantibioticssemen extenderbacterial loadcollared peccary |
| spellingShingle | Samara S. J. Moreira Caio S. Santos Thibério S. Castelo Luana G. P. Bezerra Érica C. G. Praxedes Tayná M. Matos João B. F. Souza-Junior Francisco M. C. Feijó Pierre Comizzoli Alexandre R. Silva Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries Frontiers in Veterinary Science wildlife biobank antibiotics semen extender bacterial load collared peccary |
| title | Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| title_full | Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| title_fullStr | Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| title_full_unstemmed | Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| title_short | Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| title_sort | investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries |
| topic | wildlife biobank antibiotics semen extender bacterial load collared peccary |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.954921/full |
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