Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens
The gastrointestinal tract of broilers is susceptible to oxidative stress induced by heat stress (HS) and diet, which can be mitigated by the supply of exogenous vitamins and antioxidants. The aim of this study was to compare the extent of the effects of cyclic HS, and high levels of polyunsaturated...
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MDPI AG
2023-12-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/14/1/64 |
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| author | Vida Rezar Manca Pečjak Pal Jakob Leskovec Alenka Levart Janez Salobir Andrej Lavrenčič Milka Vrecl Tatjana Pirman |
| author_facet | Vida Rezar Manca Pečjak Pal Jakob Leskovec Alenka Levart Janez Salobir Andrej Lavrenčič Milka Vrecl Tatjana Pirman |
| author_sort | Vida Rezar |
| collection | DOAJ |
| description | The gastrointestinal tract of broilers is susceptible to oxidative stress induced by heat stress (HS) and diet, which can be mitigated by the supply of exogenous vitamins and antioxidants. The aim of this study was to compare the extent of the effects of cyclic HS, and high levels of polyunsaturated fatty acids (PUFA) (HP) on gut health. It also aimed to investigate whether additional supplementation with vitamins E, C and selenium (HA) is required to support gut health under these conditions. In the present study, 192 one-day-old male Ross 308 broilers were randomly divided into eight experimental groups. Exposure to HS and HP significantly (<i>p</i> < 0.001) decreased villus height and villus-to-crypt ratio in the duodenum, while no differences were observed in the jejunum and ileum. In addition, oxidative stress in the liver, indicated by increased malondialdehyde (MDA) levels, was increased in the HP groups (<i>p</i> < 0.0001), while the HA groups had a positive effect on lowering MDA levels. The results confirm that cyclic HS and HP induce oxidative stress that damages the structure of intestinal morphology and that supplementation with HA could be a potential approach to mitigate the negative effects of these stressors. |
| first_indexed | 2024-03-08T09:59:56Z |
| format | Article |
| id | doaj.art-8852c745459e4c788ef14b36ae27ec95 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-08T09:59:56Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-8852c745459e4c788ef14b36ae27ec952024-01-29T13:39:33ZengMDPI AGAgriculture2077-04722023-12-011416410.3390/agriculture14010064Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler ChickensVida Rezar0Manca Pečjak Pal1Jakob Leskovec2Alenka Levart3Janez Salobir4Andrej Lavrenčič5Milka Vrecl6Tatjana Pirman7Department of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaInstitute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, SloveniaThe gastrointestinal tract of broilers is susceptible to oxidative stress induced by heat stress (HS) and diet, which can be mitigated by the supply of exogenous vitamins and antioxidants. The aim of this study was to compare the extent of the effects of cyclic HS, and high levels of polyunsaturated fatty acids (PUFA) (HP) on gut health. It also aimed to investigate whether additional supplementation with vitamins E, C and selenium (HA) is required to support gut health under these conditions. In the present study, 192 one-day-old male Ross 308 broilers were randomly divided into eight experimental groups. Exposure to HS and HP significantly (<i>p</i> < 0.001) decreased villus height and villus-to-crypt ratio in the duodenum, while no differences were observed in the jejunum and ileum. In addition, oxidative stress in the liver, indicated by increased malondialdehyde (MDA) levels, was increased in the HP groups (<i>p</i> < 0.0001), while the HA groups had a positive effect on lowering MDA levels. The results confirm that cyclic HS and HP induce oxidative stress that damages the structure of intestinal morphology and that supplementation with HA could be a potential approach to mitigate the negative effects of these stressors.https://www.mdpi.com/2077-0472/14/1/64cyclic heat stresspolyunsaturated fatty acidsantioxidantsoxidative stressgut fermentationshort chain fatty acids |
| spellingShingle | Vida Rezar Manca Pečjak Pal Jakob Leskovec Alenka Levart Janez Salobir Andrej Lavrenčič Milka Vrecl Tatjana Pirman Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens Agriculture cyclic heat stress polyunsaturated fatty acids antioxidants oxidative stress gut fermentation short chain fatty acids |
| title | Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens |
| title_full | Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens |
| title_fullStr | Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens |
| title_full_unstemmed | Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens |
| title_short | Combined Effects of Cyclic Heat Stress, Dietary Induced Oxidative Stress and Different Levels of Antioxidant on Gut Fermentation Activity and Mucosal Morphology in Broiler Chickens |
| title_sort | combined effects of cyclic heat stress dietary induced oxidative stress and different levels of antioxidant on gut fermentation activity and mucosal morphology in broiler chickens |
| topic | cyclic heat stress polyunsaturated fatty acids antioxidants oxidative stress gut fermentation short chain fatty acids |
| url | https://www.mdpi.com/2077-0472/14/1/64 |
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