Biological properties of domestic strain vRub-Ant of rubella virus
Introduction. Rubella is a mild infectious disease affecting mainly children and is caused by the rubella virus, part of the Matonoviridae family, genus Rubivirus. Rubella causes congenital rubella syndrome (CRS) and is the main cause of developmental abnormalities, especially blindness and deafness...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2022-12-01
|
| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/1254/768 |
| _version_ | 1828053467540750336 |
|---|---|
| author | Firaya G. Nagieva Elena P. Barkova Alexandra D. Stroeva Yulia I. Ammur Alexander V. Sidorov Olga S. Kharchenko Georgy N. Bukhtoyarov Oksana A. Svitich Vitaly V. Zverev |
| author_facet | Firaya G. Nagieva Elena P. Barkova Alexandra D. Stroeva Yulia I. Ammur Alexander V. Sidorov Olga S. Kharchenko Georgy N. Bukhtoyarov Oksana A. Svitich Vitaly V. Zverev |
| author_sort | Firaya G. Nagieva |
| collection | DOAJ |
| description | Introduction. Rubella is a mild infectious disease affecting mainly children and is caused by the rubella virus, part of the Matonoviridae family, genus Rubivirus. Rubella causes congenital rubella syndrome (CRS) and is the main cause of developmental abnormalities, especially blindness and deafness.
There is no specific treatment for rubella and CRS. In order to avoid possible complications from rubella infection, a live attenuated rubella vaccine based on the foreign strain of Wistar RA 27/3 rubella virus is used. However, the actual, more effective and preferred vaccine strain the rubella virus for the Russian Federation is considered to be a viral strain of rubella circulating on its territory.
The aim of the study was to study the biological properties of the developed domestic cold-adapted strain vRub-Ant circulating in the territory of the Russian Federation.
Materials and methods. Following cell cultures were used in the study human embryo lung diploid cell strain LECH-3, transferable cell line from embryonic kidney cells of green monkeys Vero CCL-81 and Vero ECC, human mesenchymal stem cells, human peripheral blood mononuclear cells (PBMC). Cell cultures were grown on a DMEM/F12 nutrient medium with the addition of 5% fetal bovine serum. Swabs from the pharynx and nasal passages from a child with rubella were used as clinical virus-containing material. Monoclonal anti-idiotypic antibodies m(anti-ID)Ab were used to assess the expression level of alpha/beta and gamma interferon receptors (/ and IFN-R)Ab, imitating the biological effects of alpha/beta and gamma interferons (/ and IFN) of humans. The cultural, virological, immunochemical and serological research methods were applied in the study.
Results. Attenuation of the vRub-Ant clinical isolate of rubella virus was carried out for 20 consecutive passages on LECH-3 diploid cells at a reduced temperature of 30C. The main biological markers of attenuation were determined to be ts and ca phenotypes. The avirulence of the attenuated viral strain (att-phenotype) was assessed by the level of expression of / and IFN-R. A lower level of / and IFN-R expression was found on the membranes of human PBMC induced by the vaccine strain vRub-Ant in comparison with the parent wild variant of the rubella virus. This trait,the att phenotype, is characteristic of attenuated viral strains. It has been shown that the vaccine strain vRub-Ant has lost neurotropism and was unable to bind to the membrane receptors of the brain (MRB) of guinea pig embryos, unlike its parent rubella virus strain. The high immunogenicity of the domestic cold-adapted strain vRub-Ant was confirmed by high titers of neutralizing rubella antibodies observed in guinea pigs immunized subcutaneously with one vaccination dose of the virus.
Conclusion. A domestic attenuated vaccine strain vRub-Ant of the rubella virus that has the main biological markers of attenuation (ts-ca and att phenotypes) has been developed. The vaccine strain vRub-Ant induces a high levels of neutralizing antibodies in guinea pigs following the immunization with a single vaccination dose of the vaccine. The viral strain vRub-Ant has lost its tropism to the MRB of guinea pig embryos, unlike its parent variant. |
| first_indexed | 2024-04-10T20:08:08Z |
| format | Article |
| id | doaj.art-885553af3c2042ec83eb0f18fd0b392b |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-10T20:08:08Z |
| publishDate | 2022-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-885553af3c2042ec83eb0f18fd0b392b2023-01-26T15:09:56ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132022-12-0199550551310.36233/0372-9311-320836Biological properties of domestic strain vRub-Ant of rubella virusFiraya G. Nagieva0https://orcid.org/0000-0001-8204-4899Elena P. Barkova1https://orcid.org/0000-0002-3369-8869Alexandra D. Stroeva2https://orcid.org/0000-0002-4179-931XYulia I. Ammur3https://orcid.org/0000-0003-0223-5738Alexander V. Sidorov4https://orcid.org/0000-0002-3561-8295Olga S. Kharchenko5https://orcid.org/0000-0002-2169-9610Georgy N. Bukhtoyarov6https://orcid.org/0000-0987-6885-3210Oksana A. Svitich7https://orcid.org/0000-0003-1757-8389Vitaly V. Zverev8https://orcid.org/0000-0001-5808-2246Mechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraMechnikov Research Institute for Vaccines and SeraIntroduction. Rubella is a mild infectious disease affecting mainly children and is caused by the rubella virus, part of the Matonoviridae family, genus Rubivirus. Rubella causes congenital rubella syndrome (CRS) and is the main cause of developmental abnormalities, especially blindness and deafness. There is no specific treatment for rubella and CRS. In order to avoid possible complications from rubella infection, a live attenuated rubella vaccine based on the foreign strain of Wistar RA 27/3 rubella virus is used. However, the actual, more effective and preferred vaccine strain the rubella virus for the Russian Federation is considered to be a viral strain of rubella circulating on its territory. The aim of the study was to study the biological properties of the developed domestic cold-adapted strain vRub-Ant circulating in the territory of the Russian Federation. Materials and methods. Following cell cultures were used in the study human embryo lung diploid cell strain LECH-3, transferable cell line from embryonic kidney cells of green monkeys Vero CCL-81 and Vero ECC, human mesenchymal stem cells, human peripheral blood mononuclear cells (PBMC). Cell cultures were grown on a DMEM/F12 nutrient medium with the addition of 5% fetal bovine serum. Swabs from the pharynx and nasal passages from a child with rubella were used as clinical virus-containing material. Monoclonal anti-idiotypic antibodies m(anti-ID)Ab were used to assess the expression level of alpha/beta and gamma interferon receptors (/ and IFN-R)Ab, imitating the biological effects of alpha/beta and gamma interferons (/ and IFN) of humans. The cultural, virological, immunochemical and serological research methods were applied in the study. Results. Attenuation of the vRub-Ant clinical isolate of rubella virus was carried out for 20 consecutive passages on LECH-3 diploid cells at a reduced temperature of 30C. The main biological markers of attenuation were determined to be ts and ca phenotypes. The avirulence of the attenuated viral strain (att-phenotype) was assessed by the level of expression of / and IFN-R. A lower level of / and IFN-R expression was found on the membranes of human PBMC induced by the vaccine strain vRub-Ant in comparison with the parent wild variant of the rubella virus. This trait,the att phenotype, is characteristic of attenuated viral strains. It has been shown that the vaccine strain vRub-Ant has lost neurotropism and was unable to bind to the membrane receptors of the brain (MRB) of guinea pig embryos, unlike its parent rubella virus strain. The high immunogenicity of the domestic cold-adapted strain vRub-Ant was confirmed by high titers of neutralizing rubella antibodies observed in guinea pigs immunized subcutaneously with one vaccination dose of the virus. Conclusion. A domestic attenuated vaccine strain vRub-Ant of the rubella virus that has the main biological markers of attenuation (ts-ca and att phenotypes) has been developed. The vaccine strain vRub-Ant induces a high levels of neutralizing antibodies in guinea pigs following the immunization with a single vaccination dose of the vaccine. The viral strain vRub-Ant has lost its tropism to the MRB of guinea pig embryos, unlike its parent variant.https://microbiol.crie.ru/jour/article/viewFile/1254/768rubella viruscell culturecold-adapted domestic strain of rubella virus attenuationinfectivity of the sampleimmunogenicityantigenicityneurotropicitypreparation of the membrane receptor of the brain of guinea pigs |
| spellingShingle | Firaya G. Nagieva Elena P. Barkova Alexandra D. Stroeva Yulia I. Ammur Alexander V. Sidorov Olga S. Kharchenko Georgy N. Bukhtoyarov Oksana A. Svitich Vitaly V. Zverev Biological properties of domestic strain vRub-Ant of rubella virus Журнал микробиологии, эпидемиологии и иммунобиологии rubella virus cell culture cold-adapted domestic strain of rubella virus attenuation infectivity of the sample immunogenicity antigenicity neurotropicity preparation of the membrane receptor of the brain of guinea pigs |
| title | Biological properties of domestic strain vRub-Ant of rubella virus |
| title_full | Biological properties of domestic strain vRub-Ant of rubella virus |
| title_fullStr | Biological properties of domestic strain vRub-Ant of rubella virus |
| title_full_unstemmed | Biological properties of domestic strain vRub-Ant of rubella virus |
| title_short | Biological properties of domestic strain vRub-Ant of rubella virus |
| title_sort | biological properties of domestic strain vrub ant of rubella virus |
| topic | rubella virus cell culture cold-adapted domestic strain of rubella virus attenuation infectivity of the sample immunogenicity antigenicity neurotropicity preparation of the membrane receptor of the brain of guinea pigs |
| url | https://microbiol.crie.ru/jour/article/viewFile/1254/768 |
| work_keys_str_mv | AT firayagnagieva biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT elenapbarkova biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT alexandradstroeva biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT yuliaiammur biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT alexandervsidorov biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT olgaskharchenko biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT georgynbukhtoyarov biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT oksanaasvitich biologicalpropertiesofdomesticstrainvrubantofrubellavirus AT vitalyvzverev biologicalpropertiesofdomesticstrainvrubantofrubellavirus |