Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos
Abstract Background The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic who...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-02-01
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| Series: | Environmental Sciences Europe |
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| Online Access: | http://link.springer.com/article/10.1186/s12302-019-0188-y |
| _version_ | 1828531054440349696 |
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| author | Riccardo Massei Henner Hollert Martin Krauss Wolf von Tümpling Cindy Weidauer Peter Haglund Eberhard Küster Christine Gallampois Mats Tysklind Werner Brack |
| author_facet | Riccardo Massei Henner Hollert Martin Krauss Wolf von Tümpling Cindy Weidauer Peter Haglund Eberhard Küster Christine Gallampois Mats Tysklind Werner Brack |
| author_sort | Riccardo Massei |
| collection | DOAJ |
| description | Abstract Background The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic whole mixture assessment workflow based on a slightly modified Danio rerio embryo acute toxicity test with chemical characterization. Danio rerio embryos were directly exposed to sieved sediment (≤ 63 μm) for 96 h. Sediment samples were collected from three polluted sites (Kramfors, Sundsvall and Örnsköldsvik) in the Gulf of Bothnia (Sweden) which are characterized by a long history of pulp and paper industry impact. Effect data were supported by chemical analyses of 237 organic pollutants and 30 trace elements. Results The results show that malformations and neurotoxic compounds are the main drivers of differentiation in chemical and effects analyses, respectively. Specific spinal cord malformations and delayed hatching were observed only in sediments from Kramfors while light hyperactivity was seen only after exposure to sediments from Sundsvall. Conclusions Our experiments demonstrate that specific chemical profiles lead to specific effect patterns in Danio rerio embryos. In fact, behavioral endpoints could help detect the exposure to neurotoxins, and the observation of body malformations seems to be a potential tool for the identification of site-specific pollutants as polychlorinated biphenyl (PCBs), brominated flame retardants (BFRs) and several pesticides. Overall, results show the suitability of Danio rerio embryos for the fast screening of sediment samples. |
| first_indexed | 2024-12-11T22:32:05Z |
| format | Article |
| id | doaj.art-885600502eeb4acdb55fa392054693c4 |
| institution | Directory Open Access Journal |
| issn | 2190-4707 2190-4715 |
| language | English |
| last_indexed | 2024-12-11T22:32:05Z |
| publishDate | 2019-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Environmental Sciences Europe |
| spelling | doaj.art-885600502eeb4acdb55fa392054693c42022-12-22T00:48:05ZengSpringerOpenEnvironmental Sciences Europe2190-47072190-47152019-02-0131111210.1186/s12302-019-0188-yToxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryosRiccardo Massei0Henner Hollert1Martin Krauss2Wolf von Tümpling3Cindy Weidauer4Peter Haglund5Eberhard Küster6Christine Gallampois7Mats Tysklind8Werner Brack9Department of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZDepartment of Ecosystem Analysis (ESA), Institute for Environmental Research, RWTH-Aachen UniversityDepartment of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZCentral Laboratory for Water Analytics and Chemometrics, Helmholtz Centre for Environmental Research - UFZDepartment of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZDepartment of Chemistry, Umea UniversityDepartment Bioanalytical Ecotoxicology, Helmholtz Centre for Environmental Research - UFZDepartment of Chemistry, Umea UniversityDepartment of Chemistry, Umea UniversityDepartment of Effect-Directed Analysis, Helmholtz Centre for Environmental Research - UFZAbstract Background The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic whole mixture assessment workflow based on a slightly modified Danio rerio embryo acute toxicity test with chemical characterization. Danio rerio embryos were directly exposed to sieved sediment (≤ 63 μm) for 96 h. Sediment samples were collected from three polluted sites (Kramfors, Sundsvall and Örnsköldsvik) in the Gulf of Bothnia (Sweden) which are characterized by a long history of pulp and paper industry impact. Effect data were supported by chemical analyses of 237 organic pollutants and 30 trace elements. Results The results show that malformations and neurotoxic compounds are the main drivers of differentiation in chemical and effects analyses, respectively. Specific spinal cord malformations and delayed hatching were observed only in sediments from Kramfors while light hyperactivity was seen only after exposure to sediments from Sundsvall. Conclusions Our experiments demonstrate that specific chemical profiles lead to specific effect patterns in Danio rerio embryos. In fact, behavioral endpoints could help detect the exposure to neurotoxins, and the observation of body malformations seems to be a potential tool for the identification of site-specific pollutants as polychlorinated biphenyl (PCBs), brominated flame retardants (BFRs) and several pesticides. Overall, results show the suitability of Danio rerio embryos for the fast screening of sediment samples.http://link.springer.com/article/10.1186/s12302-019-0188-yDanio rerio embryosSedimentsMonitoringNeurotoxicityBehaviorMixtures |
| spellingShingle | Riccardo Massei Henner Hollert Martin Krauss Wolf von Tümpling Cindy Weidauer Peter Haglund Eberhard Küster Christine Gallampois Mats Tysklind Werner Brack Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos Environmental Sciences Europe Danio rerio embryos Sediments Monitoring Neurotoxicity Behavior Mixtures |
| title | Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos |
| title_full | Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos |
| title_fullStr | Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos |
| title_full_unstemmed | Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos |
| title_short | Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos |
| title_sort | toxicity and neurotoxicity profiling of contaminated sediments from gulf of bothnia sweden a multi endpoint assay with zebrafish embryos |
| topic | Danio rerio embryos Sediments Monitoring Neurotoxicity Behavior Mixtures |
| url | http://link.springer.com/article/10.1186/s12302-019-0188-y |
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