Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function
Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this s...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2015-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/08964 |
| _version_ | 1828375132487286784 |
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| author | Dimitar Kostadinov Joshua R Sanes |
| author_facet | Dimitar Kostadinov Joshua R Sanes |
| author_sort | Dimitar Kostadinov |
| collection | DOAJ |
| description | Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this study, we used retinal starburst amacrine cells (SACs), critical components of a direction-selective circuit, to address this issue. In SACs, both processes are mediated by the gamma-protocadherins (Pcdhgs), a family of 22 recognition molecules. We manipulated Pcdhg expression in SACs and recorded from them and their targets, direction-selective ganglion cells (DSGCs). SACs form autapses when self-avoidance is disrupted and fail to form connections with other SACs when self/non-self discrimination is perturbed. Pcdhgs are also required to prune connections between closely spaced SACs. These alterations degrade the direction selectivity of DSGCs. Thus, self-avoidance, self/non-self discrimination, and synapse elimination are essential for proper function of a circuit that computes directional motion. |
| first_indexed | 2024-04-14T07:42:16Z |
| format | Article |
| id | doaj.art-8859c39571ff476980a08b9828861e89 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:42:16Z |
| publishDate | 2015-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-8859c39571ff476980a08b9828861e892022-12-22T02:05:27ZengeLife Sciences Publications LtdeLife2050-084X2015-07-01410.7554/eLife.08964Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit functionDimitar Kostadinov0Joshua R Sanes1Center for Brain Science, Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States; Program in Neuroscience, Harvard Medical School, Boston, United StatesCenter for Brain Science, Department of Molecular and Cellular Biology, Harvard University, Cambridge, United StatesDendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other. In some cases, these neurites interact with those of neighboring neurons, a phenomenon called self/non-self discrimination. The functional roles of these processes remain unknown. In this study, we used retinal starburst amacrine cells (SACs), critical components of a direction-selective circuit, to address this issue. In SACs, both processes are mediated by the gamma-protocadherins (Pcdhgs), a family of 22 recognition molecules. We manipulated Pcdhg expression in SACs and recorded from them and their targets, direction-selective ganglion cells (DSGCs). SACs form autapses when self-avoidance is disrupted and fail to form connections with other SACs when self/non-self discrimination is perturbed. Pcdhgs are also required to prune connections between closely spaced SACs. These alterations degrade the direction selectivity of DSGCs. Thus, self-avoidance, self/non-self discrimination, and synapse elimination are essential for proper function of a circuit that computes directional motion.https://elifesciences.org/articles/08964retinastarburst amacrine cellsynapse eliminationself-recognitiondirection selectivity |
| spellingShingle | Dimitar Kostadinov Joshua R Sanes Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function eLife retina starburst amacrine cell synapse elimination self-recognition direction selectivity |
| title | Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_full | Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_fullStr | Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_full_unstemmed | Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_short | Protocadherin-dependent dendritic self-avoidance regulates neural connectivity and circuit function |
| title_sort | protocadherin dependent dendritic self avoidance regulates neural connectivity and circuit function |
| topic | retina starburst amacrine cell synapse elimination self-recognition direction selectivity |
| url | https://elifesciences.org/articles/08964 |
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