Immune cell phenotype and function patterns across the life course in individuals from rural Uganda
BackgroundTo determine the pattern of immune cell subsets across the life span in rural sub-Saharan Africa (SSA), and to set a reference standard for cell subsets amongst Africans, we characterised the major immune cell subsets in peripheral blood including T cells, B cells, monocytes, NK cells, neu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1356635/full |
| _version_ | 1827315486186012672 |
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| author | Angela Nalwoga Angela Nalwoga Marjorie Nakibuule Romin Roshan Moses Kwizera Mbonye Wendell Miley Denise Whitby Robert Newton Robert Newton Rosemary Rochford Stephen Cose Stephen Cose |
| author_facet | Angela Nalwoga Angela Nalwoga Marjorie Nakibuule Romin Roshan Moses Kwizera Mbonye Wendell Miley Denise Whitby Robert Newton Robert Newton Rosemary Rochford Stephen Cose Stephen Cose |
| author_sort | Angela Nalwoga |
| collection | DOAJ |
| description | BackgroundTo determine the pattern of immune cell subsets across the life span in rural sub-Saharan Africa (SSA), and to set a reference standard for cell subsets amongst Africans, we characterised the major immune cell subsets in peripheral blood including T cells, B cells, monocytes, NK cells, neutrophils and eosinophils, in individuals aged 3 to 89 years from Uganda.MethodsImmune phenotypes were measured using both conventional flow cytometry in 72 individuals, and full spectrum flow cytometry in 80 individuals. Epstein-Barr virus (EBV) IFN-γ T cell responses were quantified in 332 individuals using an ELISpot assay. Full blood counts of all study participants were also obtained.ResultsThe percentages of central memory (TCM) and senescent CD4+ and CD8+ T cell subsets, effector memory (TEM) CD8+ T cells and neutrophils increased with increasing age. On the other hand, the percentages of naïve T (TN) and B (BN) cells, atypical B cells (BA), total lymphocytes, eosinophils and basophils decreased with increasing age. There was no change in CD4+ or CD8+ T effector memory RA (TEMRA) cells, exhausted T cells, NK cells and monocytes with age. Higher eosinophil and basophil percentages were observed in males compared to females. T cell function as measured by IFN-γ responses to EBV increased with increasing age, peaking at 31-55 years.ConclusionThe percentages of cell subsets differ between individuals from SSA compared to those elsewhere, perhaps reflecting a different antigenic milieu. These results serve as a reference for normal values in this population. |
| first_indexed | 2024-04-24T22:57:11Z |
| format | Article |
| id | doaj.art-885aa9a0055d40bd8db4c22e12baa4f4 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-24T22:57:11Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-885aa9a0055d40bd8db4c22e12baa4f42024-03-18T04:40:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13566351356635Immune cell phenotype and function patterns across the life course in individuals from rural UgandaAngela Nalwoga0Angela Nalwoga1Marjorie Nakibuule2Romin Roshan3Moses Kwizera Mbonye4Wendell Miley5Denise Whitby6Robert Newton7Robert Newton8Rosemary Rochford9Stephen Cose10Stephen Cose11Department of Immunology and Microbiology, University of Colorado, Aurora, CO, United StatesMedical Research Council/ Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine, Entebbe, UgandaMedical Research Council/ Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine, Entebbe, UgandaFrederick National Laboratory for Cancer Research, Viral Oncology Section, AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick, MD, United StatesMedical Research Council/ Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine, Entebbe, UgandaFrederick National Laboratory for Cancer Research, Viral Oncology Section, AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick, MD, United StatesFrederick National Laboratory for Cancer Research, Viral Oncology Section, AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc., Frederick, MD, United StatesMedical Research Council/ Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine, Entebbe, UgandaDepartment of Health Sciences, University of York, York, United KingdomDepartment of Immunology and Microbiology, University of Colorado, Aurora, CO, United StatesMedical Research Council/ Uganda Virus Research Institute and London School of Hygiene & Tropical Medicine, Entebbe, UgandaDepartment of Clinical Research, London School of Hygiene & Tropical Medicine, London, United KingdomBackgroundTo determine the pattern of immune cell subsets across the life span in rural sub-Saharan Africa (SSA), and to set a reference standard for cell subsets amongst Africans, we characterised the major immune cell subsets in peripheral blood including T cells, B cells, monocytes, NK cells, neutrophils and eosinophils, in individuals aged 3 to 89 years from Uganda.MethodsImmune phenotypes were measured using both conventional flow cytometry in 72 individuals, and full spectrum flow cytometry in 80 individuals. Epstein-Barr virus (EBV) IFN-γ T cell responses were quantified in 332 individuals using an ELISpot assay. Full blood counts of all study participants were also obtained.ResultsThe percentages of central memory (TCM) and senescent CD4+ and CD8+ T cell subsets, effector memory (TEM) CD8+ T cells and neutrophils increased with increasing age. On the other hand, the percentages of naïve T (TN) and B (BN) cells, atypical B cells (BA), total lymphocytes, eosinophils and basophils decreased with increasing age. There was no change in CD4+ or CD8+ T effector memory RA (TEMRA) cells, exhausted T cells, NK cells and monocytes with age. Higher eosinophil and basophil percentages were observed in males compared to females. T cell function as measured by IFN-γ responses to EBV increased with increasing age, peaking at 31-55 years.ConclusionThe percentages of cell subsets differ between individuals from SSA compared to those elsewhere, perhaps reflecting a different antigenic milieu. These results serve as a reference for normal values in this population.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1356635/fullimmune parametersimmune phenotypesEpstein-Barr virus T cell responsesUgandalifecourse |
| spellingShingle | Angela Nalwoga Angela Nalwoga Marjorie Nakibuule Romin Roshan Moses Kwizera Mbonye Wendell Miley Denise Whitby Robert Newton Robert Newton Rosemary Rochford Stephen Cose Stephen Cose Immune cell phenotype and function patterns across the life course in individuals from rural Uganda Frontiers in Immunology immune parameters immune phenotypes Epstein-Barr virus T cell responses Uganda lifecourse |
| title | Immune cell phenotype and function patterns across the life course in individuals from rural Uganda |
| title_full | Immune cell phenotype and function patterns across the life course in individuals from rural Uganda |
| title_fullStr | Immune cell phenotype and function patterns across the life course in individuals from rural Uganda |
| title_full_unstemmed | Immune cell phenotype and function patterns across the life course in individuals from rural Uganda |
| title_short | Immune cell phenotype and function patterns across the life course in individuals from rural Uganda |
| title_sort | immune cell phenotype and function patterns across the life course in individuals from rural uganda |
| topic | immune parameters immune phenotypes Epstein-Barr virus T cell responses Uganda lifecourse |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1356635/full |
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