Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles
This study investigated the presence, distribution, and antimicrobial resistance profiles of extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> in a dairy herd located in Northern Italy. The feces of clinically healthy calves, their mothers, and the cows treated fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/3/241 |
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| author | Martina Penati Laura Musa Laura Filippone Pavesi Alessandro Guaraglia Fernando Ulloa Paolo Moroni Renata Piccinini Maria Filippa Addis |
| author_facet | Martina Penati Laura Musa Laura Filippone Pavesi Alessandro Guaraglia Fernando Ulloa Paolo Moroni Renata Piccinini Maria Filippa Addis |
| author_sort | Martina Penati |
| collection | DOAJ |
| description | This study investigated the presence, distribution, and antimicrobial resistance profiles of extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> in a dairy herd located in Northern Italy. The feces of clinically healthy calves, their mothers, and the cows treated for mastitis, as well as water, environmental samples, and waste milk were collected and subjected to bacteriological culture on CHROMagar<sup>TM</sup> ESBL plates. A questionnaire was administered to identify risk factors. The isolates were identified as <i>E. coli</i> by MALDI-TOF MS and subjected to the double-disk synergy test (DDST) and minimal inhibitory concentration (MIC) assay. As a result, ESBL <i>E. coli</i> was isolated from the feces of 28 of 37 (75.67%) calves, the feces of 2 of 3 (66.67%) treated cows, 8 of 14 (57.15%) environmental samples, and waste milk. All ESBL isolates showed multiple resistances and were categorized as multidrug-resistant (MDR). Several risk factors for ESBL <i>E. coli</i> selection and diffusion were identified, including lack of routine cleaning of calf feeding and housing equipment, administration of waste milk to male calves, and blanket dry cow therapy. In conclusion, this study highlighted the presence of MDR, ESBL <i>E. coli</i> in the feces of most dairy calves, and their association with different sample sources. Accordingly, adding to the prudent use of antibiotics, the adoption of adequate farm hygiene and biosecurity measures might also help prevent the spread and transmission of ESBL <i>E. coli</i> within the herd. |
| first_indexed | 2024-04-24T18:36:49Z |
| format | Article |
| id | doaj.art-88616b93e59d4b89b726c5ae48d8f936 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-04-24T18:36:49Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-88616b93e59d4b89b726c5ae48d8f9362024-03-27T13:18:10ZengMDPI AGAntibiotics2079-63822024-03-0113324110.3390/antibiotics13030241Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance ProfilesMartina Penati0Laura Musa1Laura Filippone Pavesi2Alessandro Guaraglia3Fernando Ulloa4Paolo Moroni5Renata Piccinini6Maria Filippa Addis7Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Humanities and Social Sciences, University of Sassari, 07100 Sassari, ItalyEscuela de Graduados, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia 5090000, ChileDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyThis study investigated the presence, distribution, and antimicrobial resistance profiles of extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> in a dairy herd located in Northern Italy. The feces of clinically healthy calves, their mothers, and the cows treated for mastitis, as well as water, environmental samples, and waste milk were collected and subjected to bacteriological culture on CHROMagar<sup>TM</sup> ESBL plates. A questionnaire was administered to identify risk factors. The isolates were identified as <i>E. coli</i> by MALDI-TOF MS and subjected to the double-disk synergy test (DDST) and minimal inhibitory concentration (MIC) assay. As a result, ESBL <i>E. coli</i> was isolated from the feces of 28 of 37 (75.67%) calves, the feces of 2 of 3 (66.67%) treated cows, 8 of 14 (57.15%) environmental samples, and waste milk. All ESBL isolates showed multiple resistances and were categorized as multidrug-resistant (MDR). Several risk factors for ESBL <i>E. coli</i> selection and diffusion were identified, including lack of routine cleaning of calf feeding and housing equipment, administration of waste milk to male calves, and blanket dry cow therapy. In conclusion, this study highlighted the presence of MDR, ESBL <i>E. coli</i> in the feces of most dairy calves, and their association with different sample sources. Accordingly, adding to the prudent use of antibiotics, the adoption of adequate farm hygiene and biosecurity measures might also help prevent the spread and transmission of ESBL <i>E. coli</i> within the herd.https://www.mdpi.com/2079-6382/13/3/241antimicrobial resistancebiosecuritydairy calvesESBL <i>E. coli</i>multidrug-resistantwaste milk |
| spellingShingle | Martina Penati Laura Musa Laura Filippone Pavesi Alessandro Guaraglia Fernando Ulloa Paolo Moroni Renata Piccinini Maria Filippa Addis Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles Antibiotics antimicrobial resistance biosecurity dairy calves ESBL <i>E. coli</i> multidrug-resistant waste milk |
| title | Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles |
| title_full | Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles |
| title_fullStr | Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles |
| title_full_unstemmed | Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles |
| title_short | Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles |
| title_sort | multidrug resistant extended spectrum beta lactamase esbl producing i escherichia coli i in a dairy herd distribution and antimicrobial resistance profiles |
| topic | antimicrobial resistance biosecurity dairy calves ESBL <i>E. coli</i> multidrug-resistant waste milk |
| url | https://www.mdpi.com/2079-6382/13/3/241 |
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