Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives
The main active ingredients of the fruiting bodies of <i>Shiraia bambusicola</i> and <i>Rubroshiraia bambusae</i> are Hypocrellins, belonging perylenequinones with potential photodynamic activity against cancer and microbial diseases. However, the strains of <i>S. bambu...
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MDPI AG
2021-07-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/7/563 |
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| author | Xin Tong Qiu-Tong Wang Xiao-Ye Shen Cheng-Lin Hou Paul F. Cannon |
| author_facet | Xin Tong Qiu-Tong Wang Xiao-Ye Shen Cheng-Lin Hou Paul F. Cannon |
| author_sort | Xin Tong |
| collection | DOAJ |
| description | The main active ingredients of the fruiting bodies of <i>Shiraia bambusicola</i> and <i>Rubroshiraia bambusae</i> are Hypocrellins, belonging perylenequinones with potential photodynamic activity against cancer and microbial diseases. However, the strains of <i>S. bambusicola</i> and <i>R. bambusae</i> do not produce hypocrellins in culture, so resource exploitation of natural products was seriously restricted. In this study, a series of novel <i>Shiraia</i>-like fungal endophyte strains, with varying sporulation ability and synthesizing diverse secondary metabolites, was isolated from different bamboos. Based on phylogenetic analyses and morphological characteristics of the endophytes, <i>Pseudoshiraia conidialis</i> gen. et sp. nov. is proposed. The secondary metabolites of different fruiting bodies and strains have been comprehensively analyzed for the first time, finding that the endophytic strains are shown not only to produce hypocrellins, but also other perylenequinonoid compounds. It was noteworthy that the highest yield of total perylenequinone production and hypocrellin A appeared in <i>P. conidialis</i> CNUCC 1353PR (1410.13 mg/L), which was significantly higher than any other wild type <i>P. conidialis</i> strains in published reports. In view of these results, the identification of <i>Shiraia</i>-like endophytes not only confirm the phylogenetic status of similar strains, but will further assist in developing the production of valuable natural products. |
| first_indexed | 2024-03-10T09:35:27Z |
| format | Article |
| id | doaj.art-8862694820b54657830b3c26fe720e07 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T09:35:27Z |
| publishDate | 2021-07-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-8862694820b54657830b3c26fe720e072023-11-22T04:10:07ZengMDPI AGJournal of Fungi2309-608X2021-07-017756310.3390/jof7070563Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin DerivativesXin Tong0Qiu-Tong Wang1Xiao-Ye Shen2Cheng-Lin Hou3Paul F. Cannon4College of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, ChinaCollege of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, ChinaCollege of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, ChinaCollege of Life Science, Capital Normal University, Xisanhuanbeilu 105, Haidian, Beijing 100048, ChinaRoyal Botanic Gardens, Kew, Surrey TW9 3AB, UKThe main active ingredients of the fruiting bodies of <i>Shiraia bambusicola</i> and <i>Rubroshiraia bambusae</i> are Hypocrellins, belonging perylenequinones with potential photodynamic activity against cancer and microbial diseases. However, the strains of <i>S. bambusicola</i> and <i>R. bambusae</i> do not produce hypocrellins in culture, so resource exploitation of natural products was seriously restricted. In this study, a series of novel <i>Shiraia</i>-like fungal endophyte strains, with varying sporulation ability and synthesizing diverse secondary metabolites, was isolated from different bamboos. Based on phylogenetic analyses and morphological characteristics of the endophytes, <i>Pseudoshiraia conidialis</i> gen. et sp. nov. is proposed. The secondary metabolites of different fruiting bodies and strains have been comprehensively analyzed for the first time, finding that the endophytic strains are shown not only to produce hypocrellins, but also other perylenequinonoid compounds. It was noteworthy that the highest yield of total perylenequinone production and hypocrellin A appeared in <i>P. conidialis</i> CNUCC 1353PR (1410.13 mg/L), which was significantly higher than any other wild type <i>P. conidialis</i> strains in published reports. In view of these results, the identification of <i>Shiraia</i>-like endophytes not only confirm the phylogenetic status of similar strains, but will further assist in developing the production of valuable natural products.https://www.mdpi.com/2309-608X/7/7/563shiraiaceaeendophytemorphologysecondary metabolitesnew taxa |
| spellingShingle | Xin Tong Qiu-Tong Wang Xiao-Ye Shen Cheng-Lin Hou Paul F. Cannon Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives Journal of Fungi shiraiaceae endophyte morphology secondary metabolites new taxa |
| title | Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives |
| title_full | Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives |
| title_fullStr | Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives |
| title_full_unstemmed | Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives |
| title_short | Phylogenetic Position of <i>Shiraia</i>-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives |
| title_sort | phylogenetic position of i shiraia i like endophytes on bamboos and the diverse biosynthesis of hypocrellin and hypocrellin derivatives |
| topic | shiraiaceae endophyte morphology secondary metabolites new taxa |
| url | https://www.mdpi.com/2309-608X/7/7/563 |
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