Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
Abstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02652-w |
| _version_ | 1818751900603908096 |
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| author | Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert |
| author_facet | Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert |
| author_sort | Sander van Boheemen |
| collection | DOAJ |
| description | Abstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5′ and 3′ untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10−4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate. |
| first_indexed | 2024-12-18T04:42:55Z |
| format | Article |
| id | doaj.art-886636b805ea47d68fb3af0a6583c12b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:42:55Z |
| publishDate | 2017-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-886636b805ea47d68fb3af0a6583c12b2022-12-21T21:20:40ZengNature PortfolioScientific Reports2045-23222017-05-01711710.1038/s41598-017-02652-wQuasispecies composition and evolution of a typical Zika virus clinical isolate from SurinameSander van Boheemen0Ali Tas1S. Yahya Anvar2Rebecca van Grootveld3Irina C. Albulescu4Martijn P. Bauer5Mariet C. Feltkamp6Peter J. Bredenbeek7Martijn J. van Hemert8Department of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Human Genetics, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Infectious Diseases, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterDepartment of Medical Microbiology, Leiden University Medical CenterAbstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5′ and 3′ untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10−4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate.https://doi.org/10.1038/s41598-017-02652-w |
| spellingShingle | Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname Scientific Reports |
| title | Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname |
| title_full | Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname |
| title_fullStr | Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname |
| title_full_unstemmed | Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname |
| title_short | Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname |
| title_sort | quasispecies composition and evolution of a typical zika virus clinical isolate from suriname |
| url | https://doi.org/10.1038/s41598-017-02652-w |
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