Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization
The current study focuses on the optimization of bacterial growing medium composition, including the carbon and nitrogen source in different concentrations, the pH value of the medium and the temperature. Optimization was performed for four environmental bacterial isolates belonging to the genera &l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/12/2521 |
| _version_ | 1797507011983704064 |
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| author | Michał Pylak Karolina Oszust Magdalena Frąc |
| author_facet | Michał Pylak Karolina Oszust Magdalena Frąc |
| author_sort | Michał Pylak |
| collection | DOAJ |
| description | The current study focuses on the optimization of bacterial growing medium composition, including the carbon and nitrogen source in different concentrations, the pH value of the medium and the temperature. Optimization was performed for four environmental bacterial isolates belonging to the genera <i>Arthrobacter</i>, <i>Pseudomonas</i> and <i>Rhodococcus</i>, which were previously obtained from wild raspberries. These bacteria proved to be potent antagonists against certain fungal and fungal-like plant pathogens. Furthermore, three preservation methods and three sample preparation techniques were evaluated. In addition, a prebiotic supplementary blend based on previous research was tested. The research included a pot experiment to estimate the influence of bacterial cultures on the growth of plant shoots and roots, on the soil enzymatic activity and the content of macronutrients, minerals and nitrogen in the soil depending on the naturalization strategy. The best carbon and nitrogen source were chosen. The addition of a supplementary blend resulted in the increased growth of two bacterial isolates. Bacterial inoculum applied to the roots and watering resulted in increased shoot mass in objects infected with plant pathogens, although in plants without the pathogen infection, bacterial inoculum resulted in the decreased mass of plants. Naturalization strategy should be matched to the pathogens present at plantations. |
| first_indexed | 2024-03-10T04:40:39Z |
| format | Article |
| id | doaj.art-886a43fc6e134b4c8e075eb084c421d8 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T04:40:39Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-886a43fc6e134b4c8e075eb084c421d82023-11-23T03:23:11ZengMDPI AGAgronomy2073-43952021-12-011112252110.3390/agronomy11122521Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry NaturalizationMichał Pylak0Karolina Oszust1Magdalena Frąc2Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandInstitute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290 Lublin, PolandThe current study focuses on the optimization of bacterial growing medium composition, including the carbon and nitrogen source in different concentrations, the pH value of the medium and the temperature. Optimization was performed for four environmental bacterial isolates belonging to the genera <i>Arthrobacter</i>, <i>Pseudomonas</i> and <i>Rhodococcus</i>, which were previously obtained from wild raspberries. These bacteria proved to be potent antagonists against certain fungal and fungal-like plant pathogens. Furthermore, three preservation methods and three sample preparation techniques were evaluated. In addition, a prebiotic supplementary blend based on previous research was tested. The research included a pot experiment to estimate the influence of bacterial cultures on the growth of plant shoots and roots, on the soil enzymatic activity and the content of macronutrients, minerals and nitrogen in the soil depending on the naturalization strategy. The best carbon and nitrogen source were chosen. The addition of a supplementary blend resulted in the increased growth of two bacterial isolates. Bacterial inoculum applied to the roots and watering resulted in increased shoot mass in objects infected with plant pathogens, although in plants without the pathogen infection, bacterial inoculum resulted in the decreased mass of plants. Naturalization strategy should be matched to the pathogens present at plantations.https://www.mdpi.com/2073-4395/11/12/2521biopreparationorganic farmingfungal plant pathogensoptimizationpreservationpot experiment |
| spellingShingle | Michał Pylak Karolina Oszust Magdalena Frąc Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization Agronomy biopreparation organic farming fungal plant pathogens optimization preservation pot experiment |
| title | Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization |
| title_full | Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization |
| title_fullStr | Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization |
| title_full_unstemmed | Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization |
| title_short | Optimization of Growing Medium and Preservation Methods for Plant Beneficial Bacteria, and Formulating a Microbial Biopreparation for Raspberry Naturalization |
| title_sort | optimization of growing medium and preservation methods for plant beneficial bacteria and formulating a microbial biopreparation for raspberry naturalization |
| topic | biopreparation organic farming fungal plant pathogens optimization preservation pot experiment |
| url | https://www.mdpi.com/2073-4395/11/12/2521 |
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