Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC
Abstract Background Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis...
Main Authors: | , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-03-01
|
Series: | Journal of Nanobiotechnology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12951-021-00821-8 |
_version_ | 1811280857005031424 |
---|---|
author | Kai Fan Chengying Lu Gaofeng Shu Xiu-Ling Lv Enqi Qiao Nannan Zhang Minjiang Chen Jingjing Song Fazong Wu Zhongwei Zhao Xiaoling Xu Min Xu Chunmiao Chen Weibin Yang Jihong Sun Yongzhong Du Jiansong Ji |
author_facet | Kai Fan Chengying Lu Gaofeng Shu Xiu-Ling Lv Enqi Qiao Nannan Zhang Minjiang Chen Jingjing Song Fazong Wu Zhongwei Zhao Xiaoling Xu Min Xu Chunmiao Chen Weibin Yang Jihong Sun Yongzhong Du Jiansong Ji |
author_sort | Kai Fan |
collection | DOAJ |
description | Abstract Background Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis of HCC due to their relatively low sensitivity and biosafety. Thus, the development of effective MR agents is highly needed for the early diagnosis of HCC. Results Herein, we fabricated an HCC-targeted nanocomplexes containing SPIO-loaded mesoporous polydopamine (MPDA@SPIO), sialic acid (SA)-modified polyethyleneimine (SA-PEI), and alpha-fetoprotein regulated ferritin gene (AFP-Fth) which was developed for the early diagnosis of HCC. It was found that the prepared nanocomplexes (MPDA@SPIO/SA-PEI/AFP-Fth) has an excellent biocompatibility towards the liver cells. In vivo and in vivo studies revealed that the transfection of AFP-Fth gene in hepatic cells significantly upregulated the expression level of ferritin, thereby resulting in an enhanced contrast on T2-weighted images via the formed endogenous MR contrast. Conclusions The results suggested that MPDA@SPIO/SA-PEI/AFP-Fth had a superior ability to enhance the MR contrast of T2-weighted images of tumor region than the other preparations, which was due to its HCC-targeted ability and the combined T2 contrast effect of endogenous ferritin and exogenous SPIO. Our study proved that MPDA@SPIO/SA-PEI/AFP-Fth nanocomplexes could be used as an effective MR contrast agent to detect HCC in the early stage. |
first_indexed | 2024-04-13T01:23:12Z |
format | Article |
id | doaj.art-886cd0e66de4471f970483d41deb46f4 |
institution | Directory Open Access Journal |
issn | 1477-3155 |
language | English |
last_indexed | 2024-04-13T01:23:12Z |
publishDate | 2021-03-01 |
publisher | BMC |
record_format | Article |
series | Journal of Nanobiotechnology |
spelling | doaj.art-886cd0e66de4471f970483d41deb46f42022-12-22T03:08:42ZengBMCJournal of Nanobiotechnology1477-31552021-03-0119111710.1186/s12951-021-00821-8Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCCKai Fan0Chengying Lu1Gaofeng Shu2Xiu-Ling Lv3Enqi Qiao4Nannan Zhang5Minjiang Chen6Jingjing Song7Fazong Wu8Zhongwei Zhao9Xiaoling Xu10Min Xu11Chunmiao Chen12Weibin Yang13Jihong Sun14Yongzhong Du15Jiansong Ji16Department of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityDepartment of Radiology, Sir Run Shaw Affiliated Hospital, School of Medicine, Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityDepartment of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang UniversityAbstract Background Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis of HCC due to their relatively low sensitivity and biosafety. Thus, the development of effective MR agents is highly needed for the early diagnosis of HCC. Results Herein, we fabricated an HCC-targeted nanocomplexes containing SPIO-loaded mesoporous polydopamine (MPDA@SPIO), sialic acid (SA)-modified polyethyleneimine (SA-PEI), and alpha-fetoprotein regulated ferritin gene (AFP-Fth) which was developed for the early diagnosis of HCC. It was found that the prepared nanocomplexes (MPDA@SPIO/SA-PEI/AFP-Fth) has an excellent biocompatibility towards the liver cells. In vivo and in vivo studies revealed that the transfection of AFP-Fth gene in hepatic cells significantly upregulated the expression level of ferritin, thereby resulting in an enhanced contrast on T2-weighted images via the formed endogenous MR contrast. Conclusions The results suggested that MPDA@SPIO/SA-PEI/AFP-Fth had a superior ability to enhance the MR contrast of T2-weighted images of tumor region than the other preparations, which was due to its HCC-targeted ability and the combined T2 contrast effect of endogenous ferritin and exogenous SPIO. Our study proved that MPDA@SPIO/SA-PEI/AFP-Fth nanocomplexes could be used as an effective MR contrast agent to detect HCC in the early stage.https://doi.org/10.1186/s12951-021-00821-8Magnetic resonance imagingSuperparamagnetic iron oxidePolydopamineFerritin geneHepatocellular carcinoma |
spellingShingle | Kai Fan Chengying Lu Gaofeng Shu Xiu-Ling Lv Enqi Qiao Nannan Zhang Minjiang Chen Jingjing Song Fazong Wu Zhongwei Zhao Xiaoling Xu Min Xu Chunmiao Chen Weibin Yang Jihong Sun Yongzhong Du Jiansong Ji Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC Journal of Nanobiotechnology Magnetic resonance imaging Superparamagnetic iron oxide Polydopamine Ferritin gene Hepatocellular carcinoma |
title | Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC |
title_full | Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC |
title_fullStr | Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC |
title_full_unstemmed | Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC |
title_short | Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC |
title_sort | sialic acid engineered mesoporous polydopamine dual loaded with ferritin gene and spio for achieving endogenous and exogenous synergistic t2 weighted magnetic resonance imaging of hcc |
topic | Magnetic resonance imaging Superparamagnetic iron oxide Polydopamine Ferritin gene Hepatocellular carcinoma |
url | https://doi.org/10.1186/s12951-021-00821-8 |
work_keys_str_mv | AT kaifan sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT chengyinglu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT gaofengshu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT xiulinglv sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT enqiqiao sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT nannanzhang sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT minjiangchen sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT jingjingsong sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT fazongwu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT zhongweizhao sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT xiaolingxu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT minxu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT chunmiaochen sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT weibinyang sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT jihongsun sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT yongzhongdu sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc AT jiansongji sialicacidengineeredmesoporouspolydopaminedualloadedwithferritingeneandspioforachievingendogenousandexogenoussynergistict2weightedmagneticresonanceimagingofhcc |