Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites.
Coral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practice...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3202587?pdf=render |
| _version_ | 1818126789145264128 |
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| author | Carly D Kenkel Galina Aglyamova Ada Alamaru Ranjeet Bhagooli Roxana Capper Ross Cunning Amanda deVillers Joshua A Haslun Laetitia Hédouin Shashank Keshavmurthy Kristin A Kuehl Huda Mahmoud Elizabeth S McGinty Phanor H Montoya-Maya Caroline V Palmer Raffaella Pantile Juan A Sánchez Tom Schils Rachel N Silverstein Logan B Squiers Pei-Ciao Tang Tamar L Goulet Mikhail V Matz |
| author_facet | Carly D Kenkel Galina Aglyamova Ada Alamaru Ranjeet Bhagooli Roxana Capper Ross Cunning Amanda deVillers Joshua A Haslun Laetitia Hédouin Shashank Keshavmurthy Kristin A Kuehl Huda Mahmoud Elizabeth S McGinty Phanor H Montoya-Maya Caroline V Palmer Raffaella Pantile Juan A Sánchez Tom Schils Rachel N Silverstein Logan B Squiers Pei-Ciao Tang Tamar L Goulet Mikhail V Matz |
| author_sort | Carly D Kenkel |
| collection | DOAJ |
| description | Coral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practices by making it possible to assess the distribution of bleaching risks among individual reef sites. Gene expression analysis based on quantitative PCR (qPCR) can be used as a diagnostic tool to determine coral condition in situ. We evaluated the expression of 13 candidate genes during heat-light stress in a common Caribbean coral Porites astreoides, and observed strong and consistent changes in gene expression in two independent experiments. Furthermore, we found that the apparent return to baseline expression levels during a recovery phase was rapid, despite visible signs of colony bleaching. We show that the response to acute heat-light stress in P. astreoides can be monitored by measuring the difference in expression of only two genes: Hsp16 and actin. We demonstrate that this assay discriminates between corals sampled from two field sites experiencing different temperatures. We also show that the assay is applicable to an Indo-Pacific congener, P. lobata, and therefore could potentially be used to diagnose acute heat-light stress on coral reefs worldwide. |
| first_indexed | 2024-12-11T07:07:02Z |
| format | Article |
| id | doaj.art-886d001fe9d149328e6fdea9a8fdc409 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T07:07:02Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-886d001fe9d149328e6fdea9a8fdc4092022-12-22T01:16:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2691410.1371/journal.pone.0026914Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites.Carly D KenkelGalina AglyamovaAda AlamaruRanjeet BhagooliRoxana CapperRoss CunningAmanda deVillersJoshua A HaslunLaetitia HédouinShashank KeshavmurthyKristin A KuehlHuda MahmoudElizabeth S McGintyPhanor H Montoya-MayaCaroline V PalmerRaffaella PantileJuan A SánchezTom SchilsRachel N SilversteinLogan B SquiersPei-Ciao TangTamar L GouletMikhail V MatzCoral reefs are declining worldwide due to increased incidence of climate-induced coral bleaching, which will have widespread biodiversity and economic impacts. A simple method to measure the sub-bleaching level of heat-light stress experienced by corals would greatly inform reef management practices by making it possible to assess the distribution of bleaching risks among individual reef sites. Gene expression analysis based on quantitative PCR (qPCR) can be used as a diagnostic tool to determine coral condition in situ. We evaluated the expression of 13 candidate genes during heat-light stress in a common Caribbean coral Porites astreoides, and observed strong and consistent changes in gene expression in two independent experiments. Furthermore, we found that the apparent return to baseline expression levels during a recovery phase was rapid, despite visible signs of colony bleaching. We show that the response to acute heat-light stress in P. astreoides can be monitored by measuring the difference in expression of only two genes: Hsp16 and actin. We demonstrate that this assay discriminates between corals sampled from two field sites experiencing different temperatures. We also show that the assay is applicable to an Indo-Pacific congener, P. lobata, and therefore could potentially be used to diagnose acute heat-light stress on coral reefs worldwide.http://europepmc.org/articles/PMC3202587?pdf=render |
| spellingShingle | Carly D Kenkel Galina Aglyamova Ada Alamaru Ranjeet Bhagooli Roxana Capper Ross Cunning Amanda deVillers Joshua A Haslun Laetitia Hédouin Shashank Keshavmurthy Kristin A Kuehl Huda Mahmoud Elizabeth S McGinty Phanor H Montoya-Maya Caroline V Palmer Raffaella Pantile Juan A Sánchez Tom Schils Rachel N Silverstein Logan B Squiers Pei-Ciao Tang Tamar L Goulet Mikhail V Matz Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. PLoS ONE |
| title | Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. |
| title_full | Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. |
| title_fullStr | Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. |
| title_full_unstemmed | Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. |
| title_short | Development of gene expression markers of acute heat-light stress in reef-building corals of the genus Porites. |
| title_sort | development of gene expression markers of acute heat light stress in reef building corals of the genus porites |
| url | http://europepmc.org/articles/PMC3202587?pdf=render |
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