Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines
The studies concerned the expression of sulfurtransferases and cystathionine beta-synthase in six human leukemia cell lines: B cell acute lymphoblastic leukemia-B-ALL (REH cells), T cell acute lymphoblastic leukemia-T-ALL (DND-41 and MOLT-4 cells), acute myeloid leukemia—AML (MV4-11 and MOLM-14 cell...
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MDPI AG
2022-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/2/148 |
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| author | Halina Jurkowska Maria Wróbel Ewa Jasek-Gajda Leszek Rydz |
| author_facet | Halina Jurkowska Maria Wróbel Ewa Jasek-Gajda Leszek Rydz |
| author_sort | Halina Jurkowska |
| collection | DOAJ |
| description | The studies concerned the expression of sulfurtransferases and cystathionine beta-synthase in six human leukemia cell lines: B cell acute lymphoblastic leukemia-B-ALL (REH cells), T cell acute lymphoblastic leukemia-T-ALL (DND-41 and MOLT-4 cells), acute myeloid leukemia—AML (MV4-11 and MOLM-14 cells), and chronic myeloid leukemia—CML (K562 cells). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed to determine the expression of thiosulfate sulfurtransferase, 3-mercaptopyruvate sulfurtransferase, gamma-cystathionase, and cystathionine beta-synthase on the mRNA and protein level. Interestingly, we found significant differences in the mRNA and protein levels of sulfurtransferases and cystathionine beta-synthase in the studied leukemia cells. The obtained results may contribute to elucidating the significance of the differences between the studied cells in the field of sulfur compound metabolism and finding new promising ways to inhibit the proliferation of various types of leukemic cells by modulating the activity of sulfurtransferases, cystathionine beta-synthase, and, consequently, the change of intracellular level of sulfane sulfur as well as H<sub>2</sub>S and reactive oxygen species production. |
| first_indexed | 2024-03-09T22:31:01Z |
| format | Article |
| id | doaj.art-886f67201c714d7aa232149fc885c8a4 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T22:31:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-886f67201c714d7aa232149fc885c8a42023-11-23T18:56:51ZengMDPI AGBiomolecules2218-273X2022-01-0112214810.3390/biom12020148Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell LinesHalina Jurkowska0Maria Wróbel1Ewa Jasek-Gajda2Leszek Rydz3Chair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 7 Kopernika St., 31-034 Kraków, PolandChair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 7 Kopernika St., 31-034 Kraków, PolandDepartment of Histology, Faculty of Medicine, Jagiellonian University Medical College, 7 Kopernika St., 31-034 Kraków, PolandChair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University Medical College, 7 Kopernika St., 31-034 Kraków, PolandThe studies concerned the expression of sulfurtransferases and cystathionine beta-synthase in six human leukemia cell lines: B cell acute lymphoblastic leukemia-B-ALL (REH cells), T cell acute lymphoblastic leukemia-T-ALL (DND-41 and MOLT-4 cells), acute myeloid leukemia—AML (MV4-11 and MOLM-14 cells), and chronic myeloid leukemia—CML (K562 cells). Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed to determine the expression of thiosulfate sulfurtransferase, 3-mercaptopyruvate sulfurtransferase, gamma-cystathionase, and cystathionine beta-synthase on the mRNA and protein level. Interestingly, we found significant differences in the mRNA and protein levels of sulfurtransferases and cystathionine beta-synthase in the studied leukemia cells. The obtained results may contribute to elucidating the significance of the differences between the studied cells in the field of sulfur compound metabolism and finding new promising ways to inhibit the proliferation of various types of leukemic cells by modulating the activity of sulfurtransferases, cystathionine beta-synthase, and, consequently, the change of intracellular level of sulfane sulfur as well as H<sub>2</sub>S and reactive oxygen species production.https://www.mdpi.com/2218-273X/12/2/148thiosulfate sulfurtransferase3-mercaptopyruvate sulfurtransferasegamma-cystathionasecystathionine beta-synthase<span style="font-variant: small-caps">l</span>-cysteineleukemia cells |
| spellingShingle | Halina Jurkowska Maria Wróbel Ewa Jasek-Gajda Leszek Rydz Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines Biomolecules thiosulfate sulfurtransferase 3-mercaptopyruvate sulfurtransferase gamma-cystathionase cystathionine beta-synthase <span style="font-variant: small-caps">l</span>-cysteine leukemia cells |
| title | Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines |
| title_full | Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines |
| title_fullStr | Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines |
| title_full_unstemmed | Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines |
| title_short | Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines |
| title_sort | sulfurtransferases and cystathionine beta synthase expression in different human leukemia cell lines |
| topic | thiosulfate sulfurtransferase 3-mercaptopyruvate sulfurtransferase gamma-cystathionase cystathionine beta-synthase <span style="font-variant: small-caps">l</span>-cysteine leukemia cells |
| url | https://www.mdpi.com/2218-273X/12/2/148 |
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