Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects
The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxida...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/1/50 |
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| author | Sung Eun Kim Somang Choi Jae-Young Hong Kyu-Sik Shim Tae-Hoon Kim Kyeongsoon Park Suk-Ha Lee |
| author_facet | Sung Eun Kim Somang Choi Jae-Young Hong Kyu-Sik Shim Tae-Hoon Kim Kyeongsoon Park Suk-Ha Lee |
| author_sort | Sung Eun Kim |
| collection | DOAJ |
| description | The purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H<sub>2</sub>O<sub>2</sub> treatment and increased proliferation of cells after H<sub>2</sub>O<sub>2</sub> treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3-E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells. |
| first_indexed | 2024-12-20T07:51:43Z |
| format | Article |
| id | doaj.art-88713e0bc3ba4dd59f75e83adc73d196 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T07:51:43Z |
| publishDate | 2019-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-88713e0bc3ba4dd59f75e83adc73d1962022-12-21T19:47:49ZengMDPI AGNanomaterials2079-49912019-12-011015010.3390/nano10010050nano10010050Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory EffectsSung Eun Kim0Somang Choi1Jae-Young Hong2Kyu-Sik Shim3Tae-Hoon Kim4Kyeongsoon Park5Suk-Ha Lee6Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, KoreaDepartment of Orthopedic Surgery and Rare Diseases Institute, Korea University Guro Hospital, #148, Gurodong-ro, Guro-gu, Seoul 08308, KoreaDepartment of Orthopedic Surgery, Korea University Ansan Hospital, #123, Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi-do 15355, KoreaDepartment of Biomedical Science, College of Medicine, Korea University, Anam-dong, Seongbuk-gu, Seoul 02841, KoreaDepartment of Orthopedic Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, #120-1, Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul 05030, KoreaDepartment of Systems Biotechnology, College of Biotechnology and Natural Resources, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, Gyeonggi-do 17546, KoreaDepartment of Orthopedic Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, #120-1, Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul 05030, KoreaThe purpose of this study was to investigate the effects of lactoferrin (LF)-conjugated nanodiamonds (NDs) in vitro on both anti-oxidant and anti-inflammation activity as well as osteogenic promotion. The application of LF-NDs resulted in sustained release of LF for up to 7 days. In vitro anti-oxidant analyses performed using Dichlorofluorescin diacetate (DCF-DA) assay and cell proliferation studies showed that LF (50 μg)-NDs effectively scavenged the reactive oxygen species (ROS) in MC3T3-E1 cells (osteoblast-like cells) after H<sub>2</sub>O<sub>2</sub> treatment and increased proliferation of cells after H<sub>2</sub>O<sub>2</sub> treatment. Treatment of lipopolysaccharide (LPS)-induced MC3T3-E1 cells with LF-NDs suppressed levels of pro-inflammatory cytokines, including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). In addition, LF-NDs were associated with outstanding enhancement of osteogenic activity of MC3T3-E1 cells due to increased alkaline phosphatase (ALP) and calcium deposition. Our findings suggest that LF-NDs are an important substrate for alleviating ROS effects and inflammation, as well as promoting osteogenic differentiation of cells.https://www.mdpi.com/2079-4991/10/1/50nanodiamondslactoferrinanti-oxidantanti-inflammatoryosteogenic differentiation |
| spellingShingle | Sung Eun Kim Somang Choi Jae-Young Hong Kyu-Sik Shim Tae-Hoon Kim Kyeongsoon Park Suk-Ha Lee Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects Nanomaterials nanodiamonds lactoferrin anti-oxidant anti-inflammatory osteogenic differentiation |
| title | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects |
| title_full | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects |
| title_fullStr | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects |
| title_full_unstemmed | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects |
| title_short | Accelerated Osteogenic Differentiation of MC3T3-E1 Cells by Lactoferrin-Conjugated Nanodiamonds through Enhanced Anti-Oxidant and Anti-Inflammatory Effects |
| title_sort | accelerated osteogenic differentiation of mc3t3 e1 cells by lactoferrin conjugated nanodiamonds through enhanced anti oxidant and anti inflammatory effects |
| topic | nanodiamonds lactoferrin anti-oxidant anti-inflammatory osteogenic differentiation |
| url | https://www.mdpi.com/2079-4991/10/1/50 |
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