Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments.
Adipose tissue macrophages are a heterogeneous collection of classically activated (M1) and alternatively activated (M2) macrophages. Interleukin 10 (IL-10) is an anti-inflammatory cytokine, secreted by a variety of cell types including M2 macrophages. We generated a macrophage cell line stably over...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3897709?pdf=render |
| _version_ | 1818244978234621952 |
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| author | Linglin Xie Qiang Fu Teresa M Ortega Lun Zhou Dane Rasmussen Jacy O'Keefe Ke K Zhang Stephen K Chapes |
| author_facet | Linglin Xie Qiang Fu Teresa M Ortega Lun Zhou Dane Rasmussen Jacy O'Keefe Ke K Zhang Stephen K Chapes |
| author_sort | Linglin Xie |
| collection | DOAJ |
| description | Adipose tissue macrophages are a heterogeneous collection of classically activated (M1) and alternatively activated (M2) macrophages. Interleukin 10 (IL-10) is an anti-inflammatory cytokine, secreted by a variety of cell types including M2 macrophages. We generated a macrophage cell line stably overexpressing IL-10 (C2D-IL10) and analyzed the C2D-IL10 cells for several macrophage markers after exposure to adipocytes compared to C2D cells transfected with an empty vector (C2D-vector). C2D-IL10 macrophage cells expressed more CD206 when co-cultured with adipocytes than C2D-vector cells; while the co-cultured cell mixture also expressed higher levels of Il4, Il10, Il1β and Tnf. Since regular C2D cells traffic to adipose tissue after adoptive transfer, we explored the impact of constitutive IL-10 expression on C2D-IL10 macrophages in adipose tissue in vivo. Adipose tissue-isolated C2D-IL10 cells increased the percentage of CD206(+), CD301(+), CD11c(-)CD206(+) (M2) and CD11c(+)CD206(+) (M1b) on their cell surface, compared to isolated C2D-vector cells. These data suggest that the expression of IL-10 remains stable, alters the C2D-IL10 macrophage cell surface phenotype and may play a role in regulating macrophage interactions with the adipose tissue. |
| first_indexed | 2024-12-12T14:25:36Z |
| format | Article |
| id | doaj.art-8874269faff54cbcbcb432878eb22713 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T14:25:36Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8874269faff54cbcbcb432878eb227132022-12-22T00:21:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8654110.1371/journal.pone.0086541Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments.Linglin XieQiang FuTeresa M OrtegaLun ZhouDane RasmussenJacy O'KeefeKe K ZhangStephen K ChapesAdipose tissue macrophages are a heterogeneous collection of classically activated (M1) and alternatively activated (M2) macrophages. Interleukin 10 (IL-10) is an anti-inflammatory cytokine, secreted by a variety of cell types including M2 macrophages. We generated a macrophage cell line stably overexpressing IL-10 (C2D-IL10) and analyzed the C2D-IL10 cells for several macrophage markers after exposure to adipocytes compared to C2D cells transfected with an empty vector (C2D-vector). C2D-IL10 macrophage cells expressed more CD206 when co-cultured with adipocytes than C2D-vector cells; while the co-cultured cell mixture also expressed higher levels of Il4, Il10, Il1β and Tnf. Since regular C2D cells traffic to adipose tissue after adoptive transfer, we explored the impact of constitutive IL-10 expression on C2D-IL10 macrophages in adipose tissue in vivo. Adipose tissue-isolated C2D-IL10 cells increased the percentage of CD206(+), CD301(+), CD11c(-)CD206(+) (M2) and CD11c(+)CD206(+) (M1b) on their cell surface, compared to isolated C2D-vector cells. These data suggest that the expression of IL-10 remains stable, alters the C2D-IL10 macrophage cell surface phenotype and may play a role in regulating macrophage interactions with the adipose tissue.http://europepmc.org/articles/PMC3897709?pdf=render |
| spellingShingle | Linglin Xie Qiang Fu Teresa M Ortega Lun Zhou Dane Rasmussen Jacy O'Keefe Ke K Zhang Stephen K Chapes Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. PLoS ONE |
| title | Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. |
| title_full | Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. |
| title_fullStr | Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. |
| title_full_unstemmed | Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. |
| title_short | Overexpression of IL-10 in C2D macrophages promotes a macrophage phenotypic switch in adipose tissue environments. |
| title_sort | overexpression of il 10 in c2d macrophages promotes a macrophage phenotypic switch in adipose tissue environments |
| url | http://europepmc.org/articles/PMC3897709?pdf=render |
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