Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i>
A <i>Vitreoscilla</i> hemoglobin (VHb) gene was efficiently expressed by the optimization of codons and intron addition in <i>G. lucidum</i>. Expression of the VHb gene was confirmed by genome PCR, quantitative real-time PCR and carbon monoxide (CO)-difference spectrum analys...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/8/1551 |
| _version_ | 1797522856281636864 |
|---|---|
| author | Zi-Xu Wang Na Li Jun-Wei Xu |
| author_facet | Zi-Xu Wang Na Li Jun-Wei Xu |
| author_sort | Zi-Xu Wang |
| collection | DOAJ |
| description | A <i>Vitreoscilla</i> hemoglobin (VHb) gene was efficiently expressed by the optimization of codons and intron addition in <i>G. lucidum</i>. Expression of the VHb gene was confirmed by genome PCR, quantitative real-time PCR and carbon monoxide (CO)-difference spectrum analysis in the transformant. The effects of the efficient expression of VHb gene on production, monosaccharide compostion, and antioxidant activity of <i>G. lucidum</i> exopolysaccharides were studied. The maximum production of exopolysaccharides in the VHb gene-bearing transformant was 1.63 g/L, which was 1.5-fold higher than expression in the wild-type strain. Efficient expression of the VHb gene did not change the monosaccharide composition or distribution of molecular weight, but it increased the mole percentage ratio of galactose and mannose in <i>G. lucidum</i> exopolysaccharide. Exopolysaccharides from the transformant had higher scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical capacity and reducing power than those from the wild-type strain. These results may be helpful for increasing production and application of exopolysaccharides produced by <i>G. lucidum</i> fermentation. |
| first_indexed | 2024-03-10T08:35:16Z |
| format | Article |
| id | doaj.art-8875d2563595498fb395752eef788dfa |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:35:16Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-8875d2563595498fb395752eef788dfa2023-11-22T08:45:22ZengMDPI AGMicroorganisms2076-26072021-07-0198155110.3390/microorganisms9081551Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i>Zi-Xu Wang0Na Li1Jun-Wei Xu2Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Science, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, ChinaA <i>Vitreoscilla</i> hemoglobin (VHb) gene was efficiently expressed by the optimization of codons and intron addition in <i>G. lucidum</i>. Expression of the VHb gene was confirmed by genome PCR, quantitative real-time PCR and carbon monoxide (CO)-difference spectrum analysis in the transformant. The effects of the efficient expression of VHb gene on production, monosaccharide compostion, and antioxidant activity of <i>G. lucidum</i> exopolysaccharides were studied. The maximum production of exopolysaccharides in the VHb gene-bearing transformant was 1.63 g/L, which was 1.5-fold higher than expression in the wild-type strain. Efficient expression of the VHb gene did not change the monosaccharide composition or distribution of molecular weight, but it increased the mole percentage ratio of galactose and mannose in <i>G. lucidum</i> exopolysaccharide. Exopolysaccharides from the transformant had higher scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH) radical capacity and reducing power than those from the wild-type strain. These results may be helpful for increasing production and application of exopolysaccharides produced by <i>G. lucidum</i> fermentation.https://www.mdpi.com/2076-2607/9/8/1551<i>Ganoderma</i><i>Vitreoscilla</i> hemoglobinexopolysaccharideproductionmonosaccharide compositionantioxidant activity |
| spellingShingle | Zi-Xu Wang Na Li Jun-Wei Xu Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> Microorganisms <i>Ganoderma</i> <i>Vitreoscilla</i> hemoglobin exopolysaccharide production monosaccharide composition antioxidant activity |
| title | Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> |
| title_full | Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> |
| title_fullStr | Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> |
| title_full_unstemmed | Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> |
| title_short | Effects of Efficient Expression of <i>Vitreoscilla</i> Hemoglobin on Production, Monosaccharide Composition, and Antioxidant Activity of Exopolysaccharides in <i>Ganoderma lucidum</i> |
| title_sort | effects of efficient expression of i vitreoscilla i hemoglobin on production monosaccharide composition and antioxidant activity of exopolysaccharides in i ganoderma lucidum i |
| topic | <i>Ganoderma</i> <i>Vitreoscilla</i> hemoglobin exopolysaccharide production monosaccharide composition antioxidant activity |
| url | https://www.mdpi.com/2076-2607/9/8/1551 |
| work_keys_str_mv | AT zixuwang effectsofefficientexpressionofivitreoscillaihemoglobinonproductionmonosaccharidecompositionandantioxidantactivityofexopolysaccharidesiniganodermalucidumi AT nali effectsofefficientexpressionofivitreoscillaihemoglobinonproductionmonosaccharidecompositionandantioxidantactivityofexopolysaccharidesiniganodermalucidumi AT junweixu effectsofefficientexpressionofivitreoscillaihemoglobinonproductionmonosaccharidecompositionandantioxidantactivityofexopolysaccharidesiniganodermalucidumi |