Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation
The simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) was performed by integrating natural deep eutectic solvents (NADES) and ultrasound-assisted extraction (UAE). Among the eight kinds of NADES screened, choline chloride-1,2-propylene glycol was the...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S135041772300370X |
| _version_ | 1797386784309510144 |
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| author | Yuxin Gan Chenyu Wang Chenfeng Xu Pingping Zhang Shutong Chen Lei Tang Junbing Zhang Huahao Zhang Shenhua Jiang |
| author_facet | Yuxin Gan Chenyu Wang Chenfeng Xu Pingping Zhang Shutong Chen Lei Tang Junbing Zhang Huahao Zhang Shenhua Jiang |
| author_sort | Yuxin Gan |
| collection | DOAJ |
| description | The simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) was performed by integrating natural deep eutectic solvents (NADES) and ultrasound-assisted extraction (UAE). Among the eight kinds of NADES screened, choline chloride-1,2-propylene glycol was the most suitable extractant. The probe-type ultrasound-assisted NADES extraction system (pr-UAE-NADES) demonstrated higher extraction efficiency compared with plate-type ultrasound-assisted NADES extraction system (pl-UAE-NADES). Orthogonal experimental design and a modified multi-index synthetic weighted scoring method were adopted to optimize pr-UAE-NADES extraction process. The optimal extraction conditions that had a maximum synthetic weighted score of 29.46 were determined to be 25 °C for extraction temperature, 600 W for ultrasonic power, 20 min for extraction time, and 25% (w/w) for water content in NADES, leading to the maximum yields (7.39 ± 0.20 mg/g and 57.99 ± 0.91 mg/g, respectively) of crocin and geniposide. Thirty-three compounds including iridoids, carotenoids, phenolic acids, flavonoids, and triterpenes in the NADES extract were identified by LC-Q-TOF-MS2 coupled with a feature-based molecular networking workflow. The kinetics evaluation of the conjugated dienes generation on Cu2+-induced low density lipoprotein (LDL) oxidation via the four-parameter logistic regression model showed that crocin increased the lag time of LDL oxidation in a concentration-dependent manner (15 μg/mL, 30 μg/mL, 45 μg/mL) by 12.66%, 35.44%, and 73.42%, respectively. The quantitative determination for fluorescence properties alteration of the apolipoprotein B-100 exhibited that crocin effectively inhibited the fluorescence quenching of tryptophan residues and the modification of lysine residues caused by reactive aldehydes and malondialdehydes. The pr-UAE-NADES showed significant efficiency toward the simultaneous extraction of crocin and geniposide from gardenia fruits. And this study demonstrates the potential utility of gardenia fruits in developing anti-atherogenic functional food. |
| first_indexed | 2024-03-08T22:15:22Z |
| format | Article |
| id | doaj.art-88770d6efa5d4225a05bec8b27617e6d |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-08T22:15:22Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
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| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-88770d6efa5d4225a05bec8b27617e6d2023-12-19T04:16:35ZengElsevierUltrasonics Sonochemistry1350-41772023-12-01101106658Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidationYuxin Gan0Chenyu Wang1Chenfeng Xu2Pingping Zhang3Shutong Chen4Lei Tang5Junbing Zhang6Huahao Zhang7Shenhua Jiang8School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, ChinaSchool of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, ChinaSchool of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, ChinaSchool of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; Corresponding authors at: School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China (S. Jiang).School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, ChinaSchool of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, ChinaJiangxi Danxia Biotechnology Co., Ltd, Yingtan 335000, ChinaSchool of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, ChinaSchool of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, China; Jiangxi Danxia Biotechnology Co., Ltd, Yingtan 335000, China; Corresponding authors at: School of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China (S. Jiang).The simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) was performed by integrating natural deep eutectic solvents (NADES) and ultrasound-assisted extraction (UAE). Among the eight kinds of NADES screened, choline chloride-1,2-propylene glycol was the most suitable extractant. The probe-type ultrasound-assisted NADES extraction system (pr-UAE-NADES) demonstrated higher extraction efficiency compared with plate-type ultrasound-assisted NADES extraction system (pl-UAE-NADES). Orthogonal experimental design and a modified multi-index synthetic weighted scoring method were adopted to optimize pr-UAE-NADES extraction process. The optimal extraction conditions that had a maximum synthetic weighted score of 29.46 were determined to be 25 °C for extraction temperature, 600 W for ultrasonic power, 20 min for extraction time, and 25% (w/w) for water content in NADES, leading to the maximum yields (7.39 ± 0.20 mg/g and 57.99 ± 0.91 mg/g, respectively) of crocin and geniposide. Thirty-three compounds including iridoids, carotenoids, phenolic acids, flavonoids, and triterpenes in the NADES extract were identified by LC-Q-TOF-MS2 coupled with a feature-based molecular networking workflow. The kinetics evaluation of the conjugated dienes generation on Cu2+-induced low density lipoprotein (LDL) oxidation via the four-parameter logistic regression model showed that crocin increased the lag time of LDL oxidation in a concentration-dependent manner (15 μg/mL, 30 μg/mL, 45 μg/mL) by 12.66%, 35.44%, and 73.42%, respectively. The quantitative determination for fluorescence properties alteration of the apolipoprotein B-100 exhibited that crocin effectively inhibited the fluorescence quenching of tryptophan residues and the modification of lysine residues caused by reactive aldehydes and malondialdehydes. The pr-UAE-NADES showed significant efficiency toward the simultaneous extraction of crocin and geniposide from gardenia fruits. And this study demonstrates the potential utility of gardenia fruits in developing anti-atherogenic functional food.http://www.sciencedirect.com/science/article/pii/S135041772300370XNatural deep eutectic solventsGardenia fruitsUltrasound-assisted extractionModified multi-index synthetic weighted scoring methodMolecular networkingLow-density lipoprotein oxidation |
| spellingShingle | Yuxin Gan Chenyu Wang Chenfeng Xu Pingping Zhang Shutong Chen Lei Tang Junbing Zhang Huahao Zhang Shenhua Jiang Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation Ultrasonics Sonochemistry Natural deep eutectic solvents Gardenia fruits Ultrasound-assisted extraction Modified multi-index synthetic weighted scoring method Molecular networking Low-density lipoprotein oxidation |
| title | Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| title_full | Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| title_fullStr | Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| title_full_unstemmed | Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| title_short | Simultaneous extraction of crocin and geniposide from gardenia fruits (Gardenia jasminoides Ellis) by probe-type ultrasound-assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| title_sort | simultaneous extraction of crocin and geniposide from gardenia fruits gardenia jasminoides ellis by probe type ultrasound assisted natural deep eutectic solvents and their inhibition effects on low density lipoprotein oxidation |
| topic | Natural deep eutectic solvents Gardenia fruits Ultrasound-assisted extraction Modified multi-index synthetic weighted scoring method Molecular networking Low-density lipoprotein oxidation |
| url | http://www.sciencedirect.com/science/article/pii/S135041772300370X |
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