Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice
Shank2 is an abundant postsynaptic scaffolding protein implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders (ASD). Deletion of Shank2 in mice has been shown to induce social deficits, repetitive behaviors, and hyperactivity, but the identity of the cell typ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnmol.2018.00209/full |
| _version_ | 1818928963086450688 |
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| author | Seungjoon Lee Eunee Lee Ryunhee Kim Jihye Kim Suho Lee Haram Park Esther Yang Hyun Kim Eunjoon Kim Eunjoon Kim |
| author_facet | Seungjoon Lee Eunee Lee Ryunhee Kim Jihye Kim Suho Lee Haram Park Esther Yang Hyun Kim Eunjoon Kim Eunjoon Kim |
| author_sort | Seungjoon Lee |
| collection | DOAJ |
| description | Shank2 is an abundant postsynaptic scaffolding protein implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders (ASD). Deletion of Shank2 in mice has been shown to induce social deficits, repetitive behaviors, and hyperactivity, but the identity of the cell types that contribute to these phenotypes has remained unclear. Here, we report a conditional mouse line with a Shank2 deletion restricted to parvalbumin (PV)-positive neurons (Pv-Cre;Shank2fl/fl mice). These mice display moderate hyperactivity in both novel and familiar environments and enhanced self-grooming in novel, but not familiar, environments. In contrast, they showed normal levels of social interaction, anxiety-like behavior, and learning and memory. Basal brain rhythms in Pv-Cre;Shank2fl/fl mice, measured by electroencephalography, were normal, but susceptibility to pentylenetetrazole (PTZ)-induced seizures was decreased. These results suggest that Shank2 deletion in PV-positive neurons leads to hyperactivity, enhanced self-grooming and suppressed brain excitation. |
| first_indexed | 2024-12-20T03:37:15Z |
| format | Article |
| id | doaj.art-8878d0d702c14084bed80abb2dd52df4 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-12-20T03:37:15Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-8878d0d702c14084bed80abb2dd52df42022-12-21T19:54:51ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992018-06-011110.3389/fnmol.2018.00209358353Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in MiceSeungjoon Lee0Eunee Lee1Ryunhee Kim2Jihye Kim3Suho Lee4Haram Park5Esther Yang6Hyun Kim7Eunjoon Kim8Eunjoon Kim9Department of Biological Sciences, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, South KoreaCenter for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, South KoreaCenter for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon, South KoreaCenter for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon, South KoreaDepartment of Biological Sciences, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, South KoreaDepartment of Anatomy, College of Medicine, Korea University, Seoul, South KoreaDepartment of Anatomy, College of Medicine, Korea University, Seoul, South KoreaDepartment of Biological Sciences, Korea Advanced Institute for Science and Technology (KAIST), Daejeon, South KoreaCenter for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS), Daejeon, South KoreaShank2 is an abundant postsynaptic scaffolding protein implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders (ASD). Deletion of Shank2 in mice has been shown to induce social deficits, repetitive behaviors, and hyperactivity, but the identity of the cell types that contribute to these phenotypes has remained unclear. Here, we report a conditional mouse line with a Shank2 deletion restricted to parvalbumin (PV)-positive neurons (Pv-Cre;Shank2fl/fl mice). These mice display moderate hyperactivity in both novel and familiar environments and enhanced self-grooming in novel, but not familiar, environments. In contrast, they showed normal levels of social interaction, anxiety-like behavior, and learning and memory. Basal brain rhythms in Pv-Cre;Shank2fl/fl mice, measured by electroencephalography, were normal, but susceptibility to pentylenetetrazole (PTZ)-induced seizures was decreased. These results suggest that Shank2 deletion in PV-positive neurons leads to hyperactivity, enhanced self-grooming and suppressed brain excitation.https://www.frontiersin.org/article/10.3389/fnmol.2018.00209/fullautism spectrum disorderShank2parvalbuminGABAergicsocial interactionself-grooming |
| spellingShingle | Seungjoon Lee Eunee Lee Ryunhee Kim Jihye Kim Suho Lee Haram Park Esther Yang Hyun Kim Eunjoon Kim Eunjoon Kim Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice Frontiers in Molecular Neuroscience autism spectrum disorder Shank2 parvalbumin GABAergic social interaction self-grooming |
| title | Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice |
| title_full | Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice |
| title_fullStr | Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice |
| title_full_unstemmed | Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice |
| title_short | Shank2 Deletion in Parvalbumin Neurons Leads to Moderate Hyperactivity, Enhanced Self-Grooming and Suppressed Seizure Susceptibility in Mice |
| title_sort | shank2 deletion in parvalbumin neurons leads to moderate hyperactivity enhanced self grooming and suppressed seizure susceptibility in mice |
| topic | autism spectrum disorder Shank2 parvalbumin GABAergic social interaction self-grooming |
| url | https://www.frontiersin.org/article/10.3389/fnmol.2018.00209/full |
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