Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats
The objective of this study is to evaluate the acute and subacute toxicity of the ethanolic extract of Marsdenia tenacissima (MTE) leaves (family: Asclepiadaceae) in albino rats. The acute toxicity was performed where the limit dose of 5000 mg/kg body weight used. Observations were made and recorded...
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MDPI AG
2017-08-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/85/3/29 |
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| author | Mayur Porwal Najam Ali Khan Kamal Kishore Maheshwari |
| author_facet | Mayur Porwal Najam Ali Khan Kamal Kishore Maheshwari |
| author_sort | Mayur Porwal |
| collection | DOAJ |
| description | The objective of this study is to evaluate the acute and subacute toxicity of the ethanolic extract of Marsdenia tenacissima (MTE) leaves (family: Asclepiadaceae) in albino rats. The acute toxicity was performed where the limit dose of 5000 mg/kg body weight used. Observations were made and recorded for 24 h, and once daily further for a period of 14 days. The rats were weighed and various observations, like mortality, behavior, injury, or any signs of illness were conducted once daily during the period. For subacute study, four groups of 10 animals (female rats) received 10% Tween 20 in distilled water (control), and 250, 500, and 1000 mg/kg of freshly-prepared extracts, respectively, every 24 h orally for 28 days. At the end of each study, hematological analysis and biochemical parameters were evaluated. Histopathological examination of vital organs of the animals were taken for gross findings, compared to controls. There was no significant difference (p > 0.05) observed in the relative organs, body weights, hematological, biochemical parameters, and gross abnormalities, compared to the control. No mortality was recorded. Therefore, analysis of results may lead to the conclusion that the medium-term oral administration of the MTE leaves for 28 days does not cause toxicity. |
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| id | doaj.art-887a86ebc2a84781b29c760a02cc4f18 |
| institution | Directory Open Access Journal |
| issn | 2218-0532 |
| language | English |
| last_indexed | 2024-04-11T12:08:34Z |
| publishDate | 2017-08-01 |
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| series | Scientia Pharmaceutica |
| spelling | doaj.art-887a86ebc2a84781b29c760a02cc4f182022-12-22T04:24:40ZengMDPI AGScientia Pharmaceutica2218-05322017-08-018532910.3390/scipharm85030029scipharm85030029Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental RatsMayur Porwal0Najam Ali Khan1Kamal Kishore Maheshwari2Department of Pharmacology, Faculty of Pharmacy, IFTM University, Moradabad 244102, IndiaDepartment of Pharmacology, Faculty of Pharmacy, IFTM University, Moradabad 244102, IndiaDepartment of Pharmacy, M.J.P. Rohilkhand University, Bareilly 243006, IndiaThe objective of this study is to evaluate the acute and subacute toxicity of the ethanolic extract of Marsdenia tenacissima (MTE) leaves (family: Asclepiadaceae) in albino rats. The acute toxicity was performed where the limit dose of 5000 mg/kg body weight used. Observations were made and recorded for 24 h, and once daily further for a period of 14 days. The rats were weighed and various observations, like mortality, behavior, injury, or any signs of illness were conducted once daily during the period. For subacute study, four groups of 10 animals (female rats) received 10% Tween 20 in distilled water (control), and 250, 500, and 1000 mg/kg of freshly-prepared extracts, respectively, every 24 h orally for 28 days. At the end of each study, hematological analysis and biochemical parameters were evaluated. Histopathological examination of vital organs of the animals were taken for gross findings, compared to controls. There was no significant difference (p > 0.05) observed in the relative organs, body weights, hematological, biochemical parameters, and gross abnormalities, compared to the control. No mortality was recorded. Therefore, analysis of results may lead to the conclusion that the medium-term oral administration of the MTE leaves for 28 days does not cause toxicity.https://www.mdpi.com/2218-0532/85/3/29acute toxicitybiochemical analysishematological parametersMarsdenia tenacissimasubacute toxicityhistopathology |
| spellingShingle | Mayur Porwal Najam Ali Khan Kamal Kishore Maheshwari Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats Scientia Pharmaceutica acute toxicity biochemical analysis hematological parameters Marsdenia tenacissima subacute toxicity histopathology |
| title | Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats |
| title_full | Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats |
| title_fullStr | Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats |
| title_full_unstemmed | Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats |
| title_short | Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats |
| title_sort | evaluation of acute and subacute oral toxicity induced by ethanolic extract of marsdenia tenacissima leaves in experimental rats |
| topic | acute toxicity biochemical analysis hematological parameters Marsdenia tenacissima subacute toxicity histopathology |
| url | https://www.mdpi.com/2218-0532/85/3/29 |
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