Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars
Abstract Background In the context of sustainable development, yeast are one class of microorganisms foreseen for the production of oil from diverse renewable feedstocks, in particular those that do not compete with the food supply. However, their use in bulk production, such as for the production o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-019-1492-x |
| _version_ | 1817975782683705344 |
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| author | Stéphane Thomas Daniel R. A. Sanya Florian Fouchard Huu-Vang Nguyen Gotthard Kunze Cécile Neuvéglise Anne-Marie Crutz-Le Coq |
| author_facet | Stéphane Thomas Daniel R. A. Sanya Florian Fouchard Huu-Vang Nguyen Gotthard Kunze Cécile Neuvéglise Anne-Marie Crutz-Le Coq |
| author_sort | Stéphane Thomas |
| collection | DOAJ |
| description | Abstract Background In the context of sustainable development, yeast are one class of microorganisms foreseen for the production of oil from diverse renewable feedstocks, in particular those that do not compete with the food supply. However, their use in bulk production, such as for the production of biodiesel, is still not cost effective, partly due to the possible poor use of desired substrates or poor robustness in the practical bioconversion process. We investigated the natural capacity of Blastobotrys adeninivorans, a yeast already used in biotechnology, to store lipids under different conditions. Results The genotyping of seven strains showed the species to actually be composed of two different groups, one that (including the well-known strain LS3) could be reassigned to Blastobotrys raffinosifermentans. We showed that, under nitrogen limitation, strains of both species can synthesize lipids to over 20% of their dry-cell weight during shake-flask cultivation in glucose or xylose medium for 96 h. In addition, organic acids were excreted into the medium. LS3, our best lipid-producing strain, could also accumulate lipids from exogenous oleic acid, up to 38.1 ± 1.6% of its dry-cell weight, and synthesize lipids from various sugar substrates, up to 36.6 ± 0.5% when growing in cellobiose. Both species, represented by LS3 and CBS 8244T, could grow with little filamentation in the lipogenic medium from 28 to 45 °C and reached lipid titers ranging from 1.76 ± 0.28 to 3.08 ± 0.49 g/L in flasks. Under these conditions, the maximum bioconversion yield (Y FA/S = 0.093 ± 0.017) was obtained with LS3 at 37 °C. The presence of genes for predicted subunits of an ATP citrate lyase in the genome of LS3 reinforces its oleaginous character. Conclusions Blastobotrys adeninivorans and B. raffinosifermentans, which are known to be xerotolerant and genetically-tractable, are promising biotechnological yeasts of the Saccharomycotina that could be further developed through genetic engineering for the production of microbial oil. To our knowledge, this is the first report of efficient lipid storage in yeast when cultivated at a temperature above 40 °C. This paves the way to help reducing costs through consolidated bioprocessing. |
| first_indexed | 2024-04-13T21:53:53Z |
| format | Article |
| id | doaj.art-887ce7302b034f07bec3393abff61b19 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T21:53:53Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-887ce7302b034f07bec3393abff61b192022-12-22T02:28:19ZengBMCBiotechnology for Biofuels1754-68342019-06-0112111510.1186/s13068-019-1492-xBlastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugarsStéphane Thomas0Daniel R. A. Sanya1Florian Fouchard2Huu-Vang Nguyen3Gotthard Kunze4Cécile Neuvéglise5Anne-Marie Crutz-Le Coq6Micalis Institute, INRA, AgroParisTech, Université Paris-SaclayMicalis Institute, INRA, AgroParisTech, Université Paris-SaclayMicalis Institute, INRA, AgroParisTech, Université Paris-SaclayMicalis Institute, INRA, AgroParisTech, Université Paris-SaclayLeibniz Institute of Plant Genetics and Crop Plant Research (IPK)Micalis Institute, INRA, AgroParisTech, Université Paris-SaclayMicalis Institute, INRA, AgroParisTech, Université Paris-SaclayAbstract Background In the context of sustainable development, yeast are one class of microorganisms foreseen for the production of oil from diverse renewable feedstocks, in particular those that do not compete with the food supply. However, their use in bulk production, such as for the production of biodiesel, is still not cost effective, partly due to the possible poor use of desired substrates or poor robustness in the practical bioconversion process. We investigated the natural capacity of Blastobotrys adeninivorans, a yeast already used in biotechnology, to store lipids under different conditions. Results The genotyping of seven strains showed the species to actually be composed of two different groups, one that (including the well-known strain LS3) could be reassigned to Blastobotrys raffinosifermentans. We showed that, under nitrogen limitation, strains of both species can synthesize lipids to over 20% of their dry-cell weight during shake-flask cultivation in glucose or xylose medium for 96 h. In addition, organic acids were excreted into the medium. LS3, our best lipid-producing strain, could also accumulate lipids from exogenous oleic acid, up to 38.1 ± 1.6% of its dry-cell weight, and synthesize lipids from various sugar substrates, up to 36.6 ± 0.5% when growing in cellobiose. Both species, represented by LS3 and CBS 8244T, could grow with little filamentation in the lipogenic medium from 28 to 45 °C and reached lipid titers ranging from 1.76 ± 0.28 to 3.08 ± 0.49 g/L in flasks. Under these conditions, the maximum bioconversion yield (Y FA/S = 0.093 ± 0.017) was obtained with LS3 at 37 °C. The presence of genes for predicted subunits of an ATP citrate lyase in the genome of LS3 reinforces its oleaginous character. Conclusions Blastobotrys adeninivorans and B. raffinosifermentans, which are known to be xerotolerant and genetically-tractable, are promising biotechnological yeasts of the Saccharomycotina that could be further developed through genetic engineering for the production of microbial oil. To our knowledge, this is the first report of efficient lipid storage in yeast when cultivated at a temperature above 40 °C. This paves the way to help reducing costs through consolidated bioprocessing.http://link.springer.com/article/10.1186/s13068-019-1492-xMicrobial oilLipid metabolismBiotechnologySaccharomycotinaThermotoleranceOleaginous yeasts |
| spellingShingle | Stéphane Thomas Daniel R. A. Sanya Florian Fouchard Huu-Vang Nguyen Gotthard Kunze Cécile Neuvéglise Anne-Marie Crutz-Le Coq Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars Biotechnology for Biofuels Microbial oil Lipid metabolism Biotechnology Saccharomycotina Thermotolerance Oleaginous yeasts |
| title | Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| title_full | Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| title_fullStr | Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| title_full_unstemmed | Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| title_short | Blastobotrys adeninivorans and B. raffinosifermentans, two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| title_sort | blastobotrys adeninivorans and b raffinosifermentans two sibling yeast species which accumulate lipids at elevated temperatures and from diverse sugars |
| topic | Microbial oil Lipid metabolism Biotechnology Saccharomycotina Thermotolerance Oleaginous yeasts |
| url | http://link.springer.com/article/10.1186/s13068-019-1492-x |
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