Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers
Knowledge of the genetic diversity and genetic relationship is important in crop improvement. The objective of this study was to determine the genetic diversity of 31 sweetpotato genotypes and furthermore to select distantly related individuals for breeding of superior parental clones. The genotypes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/14/1802 |
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| author | Sonia I. M. Naidoo Sunette M. Laurie Assefa B. Amelework Hussein Shimelis Mark Laing |
| author_facet | Sonia I. M. Naidoo Sunette M. Laurie Assefa B. Amelework Hussein Shimelis Mark Laing |
| author_sort | Sonia I. M. Naidoo |
| collection | DOAJ |
| description | Knowledge of the genetic diversity and genetic relationship is important in crop improvement. The objective of this study was to determine the genetic diversity of 31 sweetpotato genotypes and furthermore to select distantly related individuals for breeding of superior parental clones. The genotypes (sourced from the Agricultural Research Council, South Africa) originating from Africa and American continent were genotyped using eight highly polymorphic SSR markers. The SSR markers generated a total of 83 putative alleles. The polymorphic information content (PIC) of the tested simple sequence markers varied from 0.73 to 0.91, with a mean of 0.85. At least 11 different alleles were found in 8 loci within the population, with 7 effective alleles per locus. Although high diversity was found among the genotypes, genetic distances among the genotypes were relatively low. Cluster analysis revealed the existence of three distinct genetic groups, and the clustering patterns follow to some extent the geographic origin and pedigree of the genotypes. High gene flow was observed among different sweetpotato accessions. The selected SSR markers were found to be highly polymorphic with high discriminatory power for genetic characterization studies and are useful genomic tool to complement phenotyping of sweetpotato genotypes. Two heterotic groups were found in the study. The heterotic group A was composed of 14 genotypes mainly of South African origin, while the heterotic group B consisted of 17 genotypes of American origin. The two distinct groups were important for the selection of breeding clones that were distantly related to be used as parental clones in the advancement of traits of interest. |
| first_indexed | 2024-03-09T10:13:33Z |
| format | Article |
| id | doaj.art-887e223b73b14bfc85c3bbfefe0355d9 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:13:33Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-887e223b73b14bfc85c3bbfefe0355d92023-12-01T22:35:36ZengMDPI AGPlants2223-77472022-07-011114180210.3390/plants11141802Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat MarkersSonia I. M. Naidoo0Sunette M. Laurie1Assefa B. Amelework2Hussein Shimelis3Mark Laing4Agricultural Research Council-Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaAgricultural Research Council-Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaAgricultural Research Council-Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaAfrican Centre for Crop Improvement (ACCI), School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg 3209, South AfricaAfrican Centre for Crop Improvement (ACCI), School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Pietermaritzburg 3209, South AfricaKnowledge of the genetic diversity and genetic relationship is important in crop improvement. The objective of this study was to determine the genetic diversity of 31 sweetpotato genotypes and furthermore to select distantly related individuals for breeding of superior parental clones. The genotypes (sourced from the Agricultural Research Council, South Africa) originating from Africa and American continent were genotyped using eight highly polymorphic SSR markers. The SSR markers generated a total of 83 putative alleles. The polymorphic information content (PIC) of the tested simple sequence markers varied from 0.73 to 0.91, with a mean of 0.85. At least 11 different alleles were found in 8 loci within the population, with 7 effective alleles per locus. Although high diversity was found among the genotypes, genetic distances among the genotypes were relatively low. Cluster analysis revealed the existence of three distinct genetic groups, and the clustering patterns follow to some extent the geographic origin and pedigree of the genotypes. High gene flow was observed among different sweetpotato accessions. The selected SSR markers were found to be highly polymorphic with high discriminatory power for genetic characterization studies and are useful genomic tool to complement phenotyping of sweetpotato genotypes. Two heterotic groups were found in the study. The heterotic group A was composed of 14 genotypes mainly of South African origin, while the heterotic group B consisted of 17 genotypes of American origin. The two distinct groups were important for the selection of breeding clones that were distantly related to be used as parental clones in the advancement of traits of interest.https://www.mdpi.com/2223-7747/11/14/1802genetic diversitygene flowgenotypingheterotic groupsSSR markerssweet potato |
| spellingShingle | Sonia I. M. Naidoo Sunette M. Laurie Assefa B. Amelework Hussein Shimelis Mark Laing Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers Plants genetic diversity gene flow genotyping heterotic groups SSR markers sweet potato |
| title | Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers |
| title_full | Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers |
| title_fullStr | Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers |
| title_full_unstemmed | Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers |
| title_short | Selection of Sweetpotato Parental Genotypes Using Simple Sequence Repeat Markers |
| title_sort | selection of sweetpotato parental genotypes using simple sequence repeat markers |
| topic | genetic diversity gene flow genotyping heterotic groups SSR markers sweet potato |
| url | https://www.mdpi.com/2223-7747/11/14/1802 |
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