Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape
The emergence of brassica yellow virus (BrYV) has increasingly damaged crucifer crops in China in recent years. In 2020, a large number of oilseed rape in Jiangsu showed aberrant leaf color. A combined RNA-seq and RT-PCR analysis identified BrYV as the major viral pathogen. A subsequent field survey...
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1008 |
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| author | Qi Peng Wei Li Xiaoying Zhou Chengming Sun Yan Hou Maolong Hu Sanxiong Fu Jiefu Zhang Jiban Kumar Kundu Lei Lei |
| author_facet | Qi Peng Wei Li Xiaoying Zhou Chengming Sun Yan Hou Maolong Hu Sanxiong Fu Jiefu Zhang Jiban Kumar Kundu Lei Lei |
| author_sort | Qi Peng |
| collection | DOAJ |
| description | The emergence of brassica yellow virus (BrYV) has increasingly damaged crucifer crops in China in recent years. In 2020, a large number of oilseed rape in Jiangsu showed aberrant leaf color. A combined RNA-seq and RT-PCR analysis identified BrYV as the major viral pathogen. A subsequent field survey showed that the average incidence of BrYV was 32.04%. In addition to BrYV, turnip mosaic virus (TuMV) was also frequently detected. As a result, two near full-length BrYV isolates, BrYV-814NJLH and BrYV-NJ13, were cloned. Based on the newly obtained sequences and the reported BrYV and turnip yellow virus (TuYV) isolates, a phylogenetic analysis was performed, and it was found that all BrYV isolates share a common root with TuYV. Pairwise amino acid identity analysis revealed that both P2 and P3 were conserved in BrYV. Additionally, recombination analysis revealed seven recombinant events in BrYV as TuYV. We also attempted to determine BrYV infection by quantitative leaf color index, but no significant correlation was found between the two. Systemic observations indicated that BrYV-infected plants had different symptoms, such as no symptom, purple stem base and red old leaves. Overall, our work proves that BrYV is closely related to TuYV and could be considered as an epidemic strain for oilseed rape in Jiangsu. |
| first_indexed | 2024-03-11T07:14:41Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T07:14:41Z |
| publishDate | 2023-02-01 |
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| series | Plants |
| spelling | doaj.art-887ee2bd92a746c1a2ab0fdb830ec9be2023-11-17T08:22:51ZengMDPI AGPlants2223-77472023-02-01125100810.3390/plants12051008Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed RapeQi Peng0Wei Li1Xiaoying Zhou2Chengming Sun3Yan Hou4Maolong Hu5Sanxiong Fu6Jiefu Zhang7Jiban Kumar Kundu8Lei Lei9Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaCollege of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaGuizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, ChinaKey Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaKey Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaPlant Virus and Vector Interactions-Centre for Plant Virus Research, Crop Research Institute, Drnovska 507/73, 161 06 Praha, Czech RepublicGuizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550008, ChinaThe emergence of brassica yellow virus (BrYV) has increasingly damaged crucifer crops in China in recent years. In 2020, a large number of oilseed rape in Jiangsu showed aberrant leaf color. A combined RNA-seq and RT-PCR analysis identified BrYV as the major viral pathogen. A subsequent field survey showed that the average incidence of BrYV was 32.04%. In addition to BrYV, turnip mosaic virus (TuMV) was also frequently detected. As a result, two near full-length BrYV isolates, BrYV-814NJLH and BrYV-NJ13, were cloned. Based on the newly obtained sequences and the reported BrYV and turnip yellow virus (TuYV) isolates, a phylogenetic analysis was performed, and it was found that all BrYV isolates share a common root with TuYV. Pairwise amino acid identity analysis revealed that both P2 and P3 were conserved in BrYV. Additionally, recombination analysis revealed seven recombinant events in BrYV as TuYV. We also attempted to determine BrYV infection by quantitative leaf color index, but no significant correlation was found between the two. Systemic observations indicated that BrYV-infected plants had different symptoms, such as no symptom, purple stem base and red old leaves. Overall, our work proves that BrYV is closely related to TuYV and could be considered as an epidemic strain for oilseed rape in Jiangsu.https://www.mdpi.com/2223-7747/12/5/1008BrYVTuYVoilseed rapephylogenetic treeincidenceleaf color |
| spellingShingle | Qi Peng Wei Li Xiaoying Zhou Chengming Sun Yan Hou Maolong Hu Sanxiong Fu Jiefu Zhang Jiban Kumar Kundu Lei Lei Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape Plants BrYV TuYV oilseed rape phylogenetic tree incidence leaf color |
| title | Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape |
| title_full | Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape |
| title_fullStr | Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape |
| title_full_unstemmed | Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape |
| title_short | Genetic Diversity Analysis of Brassica Yellows Virus Causing Aberrant Color Symptoms in Oilseed Rape |
| title_sort | genetic diversity analysis of brassica yellows virus causing aberrant color symptoms in oilseed rape |
| topic | BrYV TuYV oilseed rape phylogenetic tree incidence leaf color |
| url | https://www.mdpi.com/2223-7747/12/5/1008 |
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