Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth
Composites and porous scaffolds produced with biodegradable natural polymers are very promising constructs which show high biocompatibility and suitable mechanical properties, with the possibility to be functionalized with growth factors involved in bone formation. For this purpose, alginate/hydroxy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/9/12/1656 |
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| author | Silvia Sancilio Eleonora Marsich Helmut Schweikl Amelia Cataldi Marialucia Gallorini |
| author_facet | Silvia Sancilio Eleonora Marsich Helmut Schweikl Amelia Cataldi Marialucia Gallorini |
| author_sort | Silvia Sancilio |
| collection | DOAJ |
| description | Composites and porous scaffolds produced with biodegradable natural polymers are very promising constructs which show high biocompatibility and suitable mechanical properties, with the possibility to be functionalized with growth factors involved in bone formation. For this purpose, alginate/hydroxyapatite (Alg/HAp) composite scaffolds using a novel production design were successfully developed and tested for their biocompatibility and osteoconductive properties in vitro. Redox homeostasis is crucial for dental pulp stem cell (DPSC) differentiation and mineralized matrix deposition, and interleukin-6 (IL-6) was found to be involved not only in immunomodulation but also in cell proliferation and differentiation. In the present study, we evaluated molecular pathways underlying the intracellular balance between redox homeostasis and extracellular matrix mineralization of DPSCs in the presence of composite scaffolds made of alginate and nano-hydroxyapatite (Alg/HAp). Prostaglandin-2 (PGE2) and IL-6 secretion was monitored by ELISA assays, and protein expression levels were quantified by Western blotting. This work aims to demonstrate a relationship between DPSC capacity to secrete a mineralized matrix in the presence of Alg/HAp scaffolds and their immunomodulatory properties. The variation of the molecular axis Nrf2 (nuclear factor erythroid 2-related factor 2)/PGE2/IL-6 suggests a tight intracellular balance between oxidative stress responses and DPSC differentiation in the presence of Alg/HAp scaffolds. |
| first_indexed | 2024-12-20T01:46:04Z |
| format | Article |
| id | doaj.art-887fc6232b03427282a4d4d740a48950 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-20T01:46:04Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-887fc6232b03427282a4d4d740a489502022-12-21T19:57:45ZengMDPI AGNanomaterials2079-49912019-11-01912165610.3390/nano9121656nano9121656Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone IngrowthSilvia Sancilio0Eleonora Marsich1Helmut Schweikl2Amelia Cataldi3Marialucia Gallorini4Department of Medicine and Ageing Sciences, G. d’ Annunzio University, Via dei Vestini 31, 66100 Chieti-Pescara, ItalyDepartment of Medicine, Surgery and Health Sciences, Trieste University, Piazzale Europa 1, 34127 Trieste, ItalyDepartment of Conservative Dentistry and Periodontology, University Hospital Regensburg, University of Regensburg, D-93042 Regensburg, GermanyDepartment of Pharmacy, G. d’ Annunzio University, Via dei Vestini 31, 66100 Chieti-Pescara, ItalyDepartment of Pharmacy, G. d’ Annunzio University, Via dei Vestini 31, 66100 Chieti-Pescara, ItalyComposites and porous scaffolds produced with biodegradable natural polymers are very promising constructs which show high biocompatibility and suitable mechanical properties, with the possibility to be functionalized with growth factors involved in bone formation. For this purpose, alginate/hydroxyapatite (Alg/HAp) composite scaffolds using a novel production design were successfully developed and tested for their biocompatibility and osteoconductive properties in vitro. Redox homeostasis is crucial for dental pulp stem cell (DPSC) differentiation and mineralized matrix deposition, and interleukin-6 (IL-6) was found to be involved not only in immunomodulation but also in cell proliferation and differentiation. In the present study, we evaluated molecular pathways underlying the intracellular balance between redox homeostasis and extracellular matrix mineralization of DPSCs in the presence of composite scaffolds made of alginate and nano-hydroxyapatite (Alg/HAp). Prostaglandin-2 (PGE2) and IL-6 secretion was monitored by ELISA assays, and protein expression levels were quantified by Western blotting. This work aims to demonstrate a relationship between DPSC capacity to secrete a mineralized matrix in the presence of Alg/HAp scaffolds and their immunomodulatory properties. The variation of the molecular axis Nrf2 (nuclear factor erythroid 2-related factor 2)/PGE2/IL-6 suggests a tight intracellular balance between oxidative stress responses and DPSC differentiation in the presence of Alg/HAp scaffolds.https://www.mdpi.com/2079-4991/9/12/1656oxidative stressnrf2il-6mineralizationdental pulpscaffoldsalginatehydroxyapatiteimmunomodulationnanomaterials |
| spellingShingle | Silvia Sancilio Eleonora Marsich Helmut Schweikl Amelia Cataldi Marialucia Gallorini Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth Nanomaterials oxidative stress nrf2 il-6 mineralization dental pulp scaffolds alginate hydroxyapatite immunomodulation nanomaterials |
| title | Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth |
| title_full | Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth |
| title_fullStr | Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth |
| title_full_unstemmed | Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth |
| title_short | Redox Control of IL-6-Mediated Dental Pulp Stem-Cell Differentiation on Alginate/Hydroxyapatite Biocomposites for Bone Ingrowth |
| title_sort | redox control of il 6 mediated dental pulp stem cell differentiation on alginate hydroxyapatite biocomposites for bone ingrowth |
| topic | oxidative stress nrf2 il-6 mineralization dental pulp scaffolds alginate hydroxyapatite immunomodulation nanomaterials |
| url | https://www.mdpi.com/2079-4991/9/12/1656 |
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