NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment
Lymphatic metastasis is the most common form in breast cancer (BC) progression. Previously, we observed that lnc045874, a most conservative homology of Homo Sapiens NONHSAT021545 (lnc021545), miR-330-3p, and EREG may have some effects in mouse hepatocarcinoma cell lines with different lymphatic meta...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/12/7/2478 |
| _version_ | 1797607657791553536 |
|---|---|
| author | Yunkun Zhang Chunmei Guo Siwen Yang Maroua Elkharti Rui Liu Ming-Zhong Sun Shuqing Liu |
| author_facet | Yunkun Zhang Chunmei Guo Siwen Yang Maroua Elkharti Rui Liu Ming-Zhong Sun Shuqing Liu |
| author_sort | Yunkun Zhang |
| collection | DOAJ |
| description | Lymphatic metastasis is the most common form in breast cancer (BC) progression. Previously, we observed that lnc045874, a most conservative homology of Homo Sapiens NONHSAT021545 (lnc021545), miR-330-3p, and EREG may have some effects in mouse hepatocarcinoma cell lines with different lymphatic metastasis potentials. Through data from TCGA and GEO database analysis, we speculated that miR-330-3p might be a tumor promoter, while EREG could be a tumor suppressor in BC. MiR-330-3p was upregulated, while lnc021545 and EREG were downregulated in 50 BC tissues. MiR-330-3p advanced the metastatic behaviors of BC cells, whereas lnc021545 and EREG resulted in the opposite effects. The three molecules’ expressions were correlated respectively and showed that miR-330-3p targeted lnc021545 and EREG to affect their expressions. Lnc021545/miR-330-3p axis affected BC metastasis by regulating EREG in epithelial-to-mesenchymal transition. In 50 BC patients, these three molecules and their cooperation are associated with aggressive tumor phenotypes, patient outcomes, and trastuzumab therapy. We finally discovered that lnc021545, miR-330-3p, and EREG formed a multi-gene co-regulation system that affected the metastasis of BC and the cooperation reflects the synergistic effects of the three molecules, recommending that their cooperation may provide a more accurate index for anti-metastasis therapeutic and prognostic evaluation of BC. |
| first_indexed | 2024-03-11T05:33:55Z |
| format | Article |
| id | doaj.art-888745677a054bfca57aa2db20ae37c3 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-11T05:33:55Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-888745677a054bfca57aa2db20ae37c32023-11-17T16:57:40ZengMDPI AGJournal of Clinical Medicine2077-03832023-03-01127247810.3390/jcm12072478NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and TreatmentYunkun Zhang0Chunmei Guo1Siwen Yang2Maroua Elkharti3Rui Liu4Ming-Zhong Sun5Shuqing Liu6Department of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaDepartment of Biochemistry, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, ChinaLymphatic metastasis is the most common form in breast cancer (BC) progression. Previously, we observed that lnc045874, a most conservative homology of Homo Sapiens NONHSAT021545 (lnc021545), miR-330-3p, and EREG may have some effects in mouse hepatocarcinoma cell lines with different lymphatic metastasis potentials. Through data from TCGA and GEO database analysis, we speculated that miR-330-3p might be a tumor promoter, while EREG could be a tumor suppressor in BC. MiR-330-3p was upregulated, while lnc021545 and EREG were downregulated in 50 BC tissues. MiR-330-3p advanced the metastatic behaviors of BC cells, whereas lnc021545 and EREG resulted in the opposite effects. The three molecules’ expressions were correlated respectively and showed that miR-330-3p targeted lnc021545 and EREG to affect their expressions. Lnc021545/miR-330-3p axis affected BC metastasis by regulating EREG in epithelial-to-mesenchymal transition. In 50 BC patients, these three molecules and their cooperation are associated with aggressive tumor phenotypes, patient outcomes, and trastuzumab therapy. We finally discovered that lnc021545, miR-330-3p, and EREG formed a multi-gene co-regulation system that affected the metastasis of BC and the cooperation reflects the synergistic effects of the three molecules, recommending that their cooperation may provide a more accurate index for anti-metastasis therapeutic and prognostic evaluation of BC.https://www.mdpi.com/2077-0383/12/7/2478breast cancermiR-330-3plnc021545EREGcoordinated function of multi-genes |
| spellingShingle | Yunkun Zhang Chunmei Guo Siwen Yang Maroua Elkharti Rui Liu Ming-Zhong Sun Shuqing Liu NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment Journal of Clinical Medicine breast cancer miR-330-3p lnc021545 EREG coordinated function of multi-genes |
| title | NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment |
| title_full | NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment |
| title_fullStr | NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment |
| title_full_unstemmed | NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment |
| title_short | NONHSAT021545/miR-330-3p/EREG: A Cooperative Axis in Breast Cancer Prognosis and Treatment |
| title_sort | nonhsat021545 mir 330 3p ereg a cooperative axis in breast cancer prognosis and treatment |
| topic | breast cancer miR-330-3p lnc021545 EREG coordinated function of multi-genes |
| url | https://www.mdpi.com/2077-0383/12/7/2478 |
| work_keys_str_mv | AT yunkunzhang nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT chunmeiguo nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT siwenyang nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT marouaelkharti nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT ruiliu nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT mingzhongsun nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment AT shuqingliu nonhsat021545mir3303peregacooperativeaxisinbreastcancerprognosisandtreatment |