Acceleration of the Deamination of Cytosine through Photo-Crosslinking
Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures. Through substitution of the groups at the 5′-position of the cytosine, the effect of hydrophilici...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/6/298 |
| _version_ | 1797595476012302336 |
|---|---|
| author | Siddhant Sethi Yasuharu Takashima Shigetaka Nakamura Licheng Wan Nozomi Honda Kenzo Fujimoto |
| author_facet | Siddhant Sethi Yasuharu Takashima Shigetaka Nakamura Licheng Wan Nozomi Honda Kenzo Fujimoto |
| author_sort | Siddhant Sethi |
| collection | DOAJ |
| description | Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures. Through substitution of the groups at the 5′-position of the cytosine, the effect of hydrophilicity was understood. It was then used to compare the various modifications of the photo-cross-linkable moiety as well as the effect of the counter base of the cytosine to edit both DNA and RNA. Furthermore, we were able to achieve cytosine deamination at 37 °C with a half-life in the order of a few hours. |
| first_indexed | 2024-03-11T02:36:58Z |
| format | Article |
| id | doaj.art-8887be9d1e7744c0a2cd85c3f4ac0e1c |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T02:36:58Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-8887be9d1e7744c0a2cd85c3f4ac0e1c2023-11-18T09:51:39ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-05-014564687470010.3390/cimb45060298Acceleration of the Deamination of Cytosine through Photo-CrosslinkingSiddhant Sethi0Yasuharu Takashima1Shigetaka Nakamura2Licheng Wan3Nozomi Honda4Kenzo Fujimoto5Bioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanBioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanBioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanBioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanBioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanBioscience, Biotechnology, and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahi-dai 1-1, Ishikawa, Nomi 923-1292, JapanHerein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures. Through substitution of the groups at the 5′-position of the cytosine, the effect of hydrophilicity was understood. It was then used to compare the various modifications of the photo-cross-linkable moiety as well as the effect of the counter base of the cytosine to edit both DNA and RNA. Furthermore, we were able to achieve cytosine deamination at 37 °C with a half-life in the order of a few hours.https://www.mdpi.com/1467-3045/45/6/298DNA manipulationcytosine deaminationphoto-crosslinkingnucleobase editingRNA editing |
| spellingShingle | Siddhant Sethi Yasuharu Takashima Shigetaka Nakamura Licheng Wan Nozomi Honda Kenzo Fujimoto Acceleration of the Deamination of Cytosine through Photo-Crosslinking Current Issues in Molecular Biology DNA manipulation cytosine deamination photo-crosslinking nucleobase editing RNA editing |
| title | Acceleration of the Deamination of Cytosine through Photo-Crosslinking |
| title_full | Acceleration of the Deamination of Cytosine through Photo-Crosslinking |
| title_fullStr | Acceleration of the Deamination of Cytosine through Photo-Crosslinking |
| title_full_unstemmed | Acceleration of the Deamination of Cytosine through Photo-Crosslinking |
| title_short | Acceleration of the Deamination of Cytosine through Photo-Crosslinking |
| title_sort | acceleration of the deamination of cytosine through photo crosslinking |
| topic | DNA manipulation cytosine deamination photo-crosslinking nucleobase editing RNA editing |
| url | https://www.mdpi.com/1467-3045/45/6/298 |
| work_keys_str_mv | AT siddhantsethi accelerationofthedeaminationofcytosinethroughphotocrosslinking AT yasuharutakashima accelerationofthedeaminationofcytosinethroughphotocrosslinking AT shigetakanakamura accelerationofthedeaminationofcytosinethroughphotocrosslinking AT lichengwan accelerationofthedeaminationofcytosinethroughphotocrosslinking AT nozomihonda accelerationofthedeaminationofcytosinethroughphotocrosslinking AT kenzofujimoto accelerationofthedeaminationofcytosinethroughphotocrosslinking |