Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains
ABSTRACT Kin discrimination in nature is an effective way for bacteria to stabilize population cooperation and maintain progeny benefits. However, so far, the research on kin discrimination for Bacillus still has concentrated on “attack and defense” between cells and diffusion-dependent molecular si...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-12-01
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| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00778-22 |
| _version_ | 1797979876825759744 |
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| author | Yan Liu Rong Huang Yuqi Chen Youzhi Miao Polonca Štefanič Ines Mandic-Mulec Ruifu Zhang Qirong Shen Zhihui Xu |
| author_facet | Yan Liu Rong Huang Yuqi Chen Youzhi Miao Polonca Štefanič Ines Mandic-Mulec Ruifu Zhang Qirong Shen Zhihui Xu |
| author_sort | Yan Liu |
| collection | DOAJ |
| description | ABSTRACT Kin discrimination in nature is an effective way for bacteria to stabilize population cooperation and maintain progeny benefits. However, so far, the research on kin discrimination for Bacillus still has concentrated on “attack and defense” between cells and diffusion-dependent molecular signals of quorum sensing, kin recognition in Bacillus, however, has not been reported. To determine whether flagellar is involve in the kin recognition of Bacillus, we constructed Bacillus velezensis SQR9 assembled with flagellin of its kin and non-kin strains, and performed a swarm boundary assay with SQR9, then analyzed sequence variation of flagellin and other flagellar structural proteins in B. velezensis genus. Our results showed that SQR9 assembled with flagellin of non-kin strains was more likely to form a border phenotype with wild-type strain SQR9 in swarm assay than that of kin strains, and that non-kin strains had greater variation in flagellin than kin strains. In B. velezensis, these variations in flagellin were prevalent and had evolved significantly faster than other flagellar structural proteins. Therefore, we proposed that flagellin is an effective tool partly involved in the kin recognition of B. velezensis strains. IMPORTANCE Kin selection plays an important role in stabilizing population cooperation and maintaining the progeny benefits for bacteria in nature. However, to date, the role of flagellin in kin recognition in Bacillus has not been reported. By using rhizospheric Bacillus velezensis SQR9, we accomplished flagellin region interchange among its related strains, and show that flagellin acts as a mediator to distinguish kin from non-kin in B. velezensis. We demonstrated the polymorphism of flagellin in B. velezensis through alignment analysis of flagellin protein sequences. Therefore, it was proposed that flagellin was likely to be an effective tool for mediating kin recognition in B. velezensis. |
| first_indexed | 2024-04-11T05:46:49Z |
| format | Article |
| id | doaj.art-88885c2135d04ae19cd689727ddfe2ff |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-04-11T05:46:49Z |
| publishDate | 2022-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-88885c2135d04ae19cd689727ddfe2ff2022-12-22T04:42:13ZengAmerican Society for MicrobiologymSystems2379-50772022-12-017610.1128/msystems.00778-22Involvement of Flagellin in Kin Recognition between Bacillus velezensis StrainsYan Liu0Rong Huang1Yuqi Chen2Youzhi Miao3Polonca Štefanič4Ines Mandic-Mulec5Ruifu Zhang6Qirong Shen7Zhihui Xu8Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaDepartment of Microbiology, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaDepartment of Microbiology, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaJiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Nanjing Agricultural University, Nanjing, Jiangsu, Peoples R ChinaABSTRACT Kin discrimination in nature is an effective way for bacteria to stabilize population cooperation and maintain progeny benefits. However, so far, the research on kin discrimination for Bacillus still has concentrated on “attack and defense” between cells and diffusion-dependent molecular signals of quorum sensing, kin recognition in Bacillus, however, has not been reported. To determine whether flagellar is involve in the kin recognition of Bacillus, we constructed Bacillus velezensis SQR9 assembled with flagellin of its kin and non-kin strains, and performed a swarm boundary assay with SQR9, then analyzed sequence variation of flagellin and other flagellar structural proteins in B. velezensis genus. Our results showed that SQR9 assembled with flagellin of non-kin strains was more likely to form a border phenotype with wild-type strain SQR9 in swarm assay than that of kin strains, and that non-kin strains had greater variation in flagellin than kin strains. In B. velezensis, these variations in flagellin were prevalent and had evolved significantly faster than other flagellar structural proteins. Therefore, we proposed that flagellin is an effective tool partly involved in the kin recognition of B. velezensis strains. IMPORTANCE Kin selection plays an important role in stabilizing population cooperation and maintaining the progeny benefits for bacteria in nature. However, to date, the role of flagellin in kin recognition in Bacillus has not been reported. By using rhizospheric Bacillus velezensis SQR9, we accomplished flagellin region interchange among its related strains, and show that flagellin acts as a mediator to distinguish kin from non-kin in B. velezensis. We demonstrated the polymorphism of flagellin in B. velezensis through alignment analysis of flagellin protein sequences. Therefore, it was proposed that flagellin was likely to be an effective tool for mediating kin recognition in B. velezensis.https://journals.asm.org/doi/10.1128/msystems.00778-22Bacillus velezensiskin recognitionbacterial flagellumvariation of flagellin sequencesflagellin tertiary structure predictionvariation of hag gene |
| spellingShingle | Yan Liu Rong Huang Yuqi Chen Youzhi Miao Polonca Štefanič Ines Mandic-Mulec Ruifu Zhang Qirong Shen Zhihui Xu Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains mSystems Bacillus velezensis kin recognition bacterial flagellum variation of flagellin sequences flagellin tertiary structure prediction variation of hag gene |
| title | Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains |
| title_full | Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains |
| title_fullStr | Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains |
| title_full_unstemmed | Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains |
| title_short | Involvement of Flagellin in Kin Recognition between Bacillus velezensis Strains |
| title_sort | involvement of flagellin in kin recognition between bacillus velezensis strains |
| topic | Bacillus velezensis kin recognition bacterial flagellum variation of flagellin sequences flagellin tertiary structure prediction variation of hag gene |
| url | https://journals.asm.org/doi/10.1128/msystems.00778-22 |
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