D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor
D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino aci...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2007-10-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/7/10/2484/ |
| _version_ | 1811186239956582400 |
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| author | Sabato D’Auria Annalisa Vitale Luigi Zeni Tullio Labella Pierangelo Orlando Giuseppe Ruggiero Maria Staiano Micaela Tartaglia Luisa Iozzino Paola Ringhieri Nunzio Cennamo Vincenzo Aurilia Viviana Scognamiglio |
| author_facet | Sabato D’Auria Annalisa Vitale Luigi Zeni Tullio Labella Pierangelo Orlando Giuseppe Ruggiero Maria Staiano Micaela Tartaglia Luisa Iozzino Paola Ringhieri Nunzio Cennamo Vincenzo Aurilia Viviana Scognamiglio |
| author_sort | Sabato D’Auria |
| collection | DOAJ |
| description | D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care. |
| first_indexed | 2024-04-11T13:42:09Z |
| format | Article |
| id | doaj.art-8888de6866494fc4be3c1a8bd826cc48 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:42:09Z |
| publishDate | 2007-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8888de6866494fc4be3c1a8bd826cc482022-12-22T04:21:13ZengMDPI AGSensors1424-82202007-10-017102484249110.3390/s7102484D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence BiosensorSabato D’AuriaAnnalisa VitaleLuigi ZeniTullio LabellaPierangelo OrlandoGiuseppe RuggieroMaria StaianoMicaela TartagliaLuisa IozzinoPaola RinghieriNunzio CennamoVincenzo AuriliaViviana ScognamiglioD-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.http://www.mdpi.com/1424-8220/7/10/2484/BiosensorOptical sensingGlucoseDiabetesFluorescenceProteins |
| spellingShingle | Sabato D’Auria Annalisa Vitale Luigi Zeni Tullio Labella Pierangelo Orlando Giuseppe Ruggiero Maria Staiano Micaela Tartaglia Luisa Iozzino Paola Ringhieri Nunzio Cennamo Vincenzo Aurilia Viviana Scognamiglio D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor Sensors Biosensor Optical sensing Glucose Diabetes Fluorescence Proteins |
| title | D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor |
| title_full | D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor |
| title_fullStr | D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor |
| title_full_unstemmed | D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor |
| title_short | D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor |
| title_sort | d galactose d glucose binding protein from escherichia coli as probe for a non consuming glucose implantable fluorescence biosensor |
| topic | Biosensor Optical sensing Glucose Diabetes Fluorescence Proteins |
| url | http://www.mdpi.com/1424-8220/7/10/2484/ |
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