Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells
Incompatible strains of Acidovorax avenae elicit an immune response in cultured rice cells, with immunity specifically induced by the flagellin of the incompatible strain. To identify genes regulated by flagellin perception signaling in cultured rice cells, gene expression patterns were analyzed wit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2004-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.986 |
| _version_ | 1818647539340017664 |
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| author | Satsuki Fujiwara Noriko Tanaka Takashi Kaneda Seiji Takayama Akira Isogai Fang-Sik Che |
| author_facet | Satsuki Fujiwara Noriko Tanaka Takashi Kaneda Seiji Takayama Akira Isogai Fang-Sik Che |
| author_sort | Satsuki Fujiwara |
| collection | DOAJ |
| description | Incompatible strains of Acidovorax avenae elicit an immune response in cultured rice cells, with immunity specifically induced by the flagellin of the incompatible strain. To identify genes regulated by flagellin perception signaling in cultured rice cells, gene expression patterns were analyzed with rice cDNA microarrays, including 3,353 independent rice cDNA clones. In all, 131 genes were differentially expressed between incompatible and compatible interactions. K-means clustering showed that 94 genes were upregulated and 32 genes were downregulated during incompatible interactions, whereas only 5 genes were upregulated during compatible interactions. Among the 126 genes that were up- or downregulated during incompatible interactions, expression of 46 genes was decreased when cultured rice cells were inoculated with a flagellin-deficient incompatible strain (Δfla1141-2), indicating that approximately 37% of the 126 genes were directly controlled by flagellin perception. Real-time reverse-transcription polymerase chain reaction analysis using flagellins purified from incompatible or compatible strains was performed to confirm flagellin-regulated expression of candidate genes selected by microarray analysis. Results showed that induction of some genes involved in the immune response is regulated not only by the flagellin perception pathway, but also by another recognition molecule-perception pathway. |
| first_indexed | 2024-12-17T01:04:08Z |
| format | Article |
| id | doaj.art-888a9e2a00704d29af21cd7a5615eac8 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T01:04:08Z |
| publishDate | 2004-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-888a9e2a00704d29af21cd7a5615eac82022-12-21T22:09:20ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062004-09-0117998699810.1094/MPMI.2004.17.9.986Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice CellsSatsuki FujiwaraNoriko TanakaTakashi KanedaSeiji TakayamaAkira IsogaiFang-Sik CheIncompatible strains of Acidovorax avenae elicit an immune response in cultured rice cells, with immunity specifically induced by the flagellin of the incompatible strain. To identify genes regulated by flagellin perception signaling in cultured rice cells, gene expression patterns were analyzed with rice cDNA microarrays, including 3,353 independent rice cDNA clones. In all, 131 genes were differentially expressed between incompatible and compatible interactions. K-means clustering showed that 94 genes were upregulated and 32 genes were downregulated during incompatible interactions, whereas only 5 genes were upregulated during compatible interactions. Among the 126 genes that were up- or downregulated during incompatible interactions, expression of 46 genes was decreased when cultured rice cells were inoculated with a flagellin-deficient incompatible strain (Δfla1141-2), indicating that approximately 37% of the 126 genes were directly controlled by flagellin perception. Real-time reverse-transcription polymerase chain reaction analysis using flagellins purified from incompatible or compatible strains was performed to confirm flagellin-regulated expression of candidate genes selected by microarray analysis. Results showed that induction of some genes involved in the immune response is regulated not only by the flagellin perception pathway, but also by another recognition molecule-perception pathway.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.986elicitor |
| spellingShingle | Satsuki Fujiwara Noriko Tanaka Takashi Kaneda Seiji Takayama Akira Isogai Fang-Sik Che Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells Molecular Plant-Microbe Interactions elicitor |
| title | Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells |
| title_full | Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells |
| title_fullStr | Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells |
| title_full_unstemmed | Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells |
| title_short | Rice cDNA Microarray-Based Gene Expression Profiling of the Response to Flagellin Perception in Cultured Rice Cells |
| title_sort | rice cdna microarray based gene expression profiling of the response to flagellin perception in cultured rice cells |
| topic | elicitor |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2004.17.9.986 |
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