Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i>
Premise of the study: Microsatellite primers were developed in the neotropical tree species <i>Cariniana legalis</i> (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. Methods and Results: We identified 96 clones containing 82 repeat motifs from a geno...
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| Format: | Article |
| Language: | English |
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Wiley
2013-06-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1200493 |
| _version_ | 1828386854136709120 |
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| author | Evandro Vagner Tambarussi Alexandre Magno Sebbenn Maria Andréia Moreno Elza Martins Ferraz Paulo Yoshio Kageyama Roland Vencovsky |
| author_facet | Evandro Vagner Tambarussi Alexandre Magno Sebbenn Maria Andréia Moreno Elza Martins Ferraz Paulo Yoshio Kageyama Roland Vencovsky |
| author_sort | Evandro Vagner Tambarussi |
| collection | DOAJ |
| description | Premise of the study: Microsatellite primers were developed in the neotropical tree species <i>Cariniana legalis</i> (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow.
Methods and Results: We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 <i>C. legalis</i> specimens and 26 <i>C. estrellensis</i> specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in <i>C. legalis</i> and three to 12 in <i>C. estrellensis</i>. For <i>C. legalis</i>, the observed (Ho) and expected (He) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For <i>C. estrellensis</i>, Ho and He ranged from 0 to 0.96 and from 0.14 to 0.91, respectively.
Conclusions: The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in <i>C. legalis</i> and the related species <i>C. estrellensis</i>. |
| first_indexed | 2024-12-10T05:45:35Z |
| format | Article |
| id | doaj.art-888b10e22cc84cfbb28f72ca6544dd32 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-10T05:45:35Z |
| publishDate | 2013-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-888b10e22cc84cfbb28f72ca6544dd322022-12-22T02:00:11ZengWileyApplications in Plant Sciences2168-04502013-06-0116120049310.3732/apps.1200493Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i>Evandro Vagner Tambarussi0Alexandre Magno Sebbenn1Maria Andréia Moreno2Elza Martins Ferraz3Paulo Yoshio Kageyama4Roland Vencovsky5Escola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, BrazilInstituto Florestal de São Paulo, CP 1322, São Paulo, São Paulo 01059-970, BrazilEscola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, BrazilEscola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, BrazilEscola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, BrazilEscola Superior de Agricultura “Luiz de Queiroz,” Universidade de São Paulo, Av. Pádua Dias 11, CP 9, Piracicaba, São Paulo 13418-900, BrazilPremise of the study: Microsatellite primers were developed in the neotropical tree species <i>Cariniana legalis</i> (Lecythidaceae) to investigate its genetic diversity, mating system, and gene flow. Methods and Results: We identified 96 clones containing 82 repeat motifs from a genomic library enriched for (CT)8 and (GT)8 motifs. Primer pairs were developed for 13 microsatellite loci and validated in 51 <i>C. legalis</i> specimens and 26 <i>C. estrellensis</i> specimens. Eleven loci were polymorphic, revealing a maximum of two to 15 alleles per locus in <i>C. legalis</i> and three to 12 in <i>C. estrellensis</i>. For <i>C. legalis</i>, the observed (Ho) and expected (He) heterozygosities ranged from 0 to 0.99 and from 0.07 to 0.90, respectively. For <i>C. estrellensis</i>, Ho and He ranged from 0 to 0.96 and from 0.14 to 0.91, respectively. Conclusions: The primers identified polymorphic loci that are suitable to study genetic diversity and structure, mating system, and gene flow in <i>C. legalis</i> and the related species <i>C. estrellensis</i>.http://www.bioone.org/doi/full/10.3732/apps.1200493Brazilian Atlantic forestCariniana legalisconservation geneticsmicrosatellite markerspopulation geneticstropical tree species |
| spellingShingle | Evandro Vagner Tambarussi Alexandre Magno Sebbenn Maria Andréia Moreno Elza Martins Ferraz Paulo Yoshio Kageyama Roland Vencovsky Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> Applications in Plant Sciences Brazilian Atlantic forest Cariniana legalis conservation genetics microsatellite markers population genetics tropical tree species |
| title | Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> |
| title_full | Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> |
| title_fullStr | Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> |
| title_full_unstemmed | Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> |
| title_short | Microsatellite Markers for <i>Cariniana legalis</i> (Lecythidaceae) and Their Transferability to <i>C. estrellensis</i> |
| title_sort | microsatellite markers for i cariniana legalis i lecythidaceae and their transferability to i c estrellensis i |
| topic | Brazilian Atlantic forest Cariniana legalis conservation genetics microsatellite markers population genetics tropical tree species |
| url | http://www.bioone.org/doi/full/10.3732/apps.1200493 |
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