Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels
Current limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanopart...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/3/160 |
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| author | Norbert Halfter Eva Espinosa-Cano Gloria María Pontes-Quero Rosa Ana Ramírez-Jiménez Christiane Heinemann Stephanie Möller Matthias Schnabelrauch Hans-Peter Wiesmann Vera Hintze Maria Rosa Aguilar |
| author_facet | Norbert Halfter Eva Espinosa-Cano Gloria María Pontes-Quero Rosa Ana Ramírez-Jiménez Christiane Heinemann Stephanie Möller Matthias Schnabelrauch Hans-Peter Wiesmann Vera Hintze Maria Rosa Aguilar |
| author_sort | Norbert Halfter |
| collection | DOAJ |
| description | Current limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanoparticles (KT NPs) can reduce pro-inflammatory markers of macrophages and increase anti-inflammatory cytokines. To assess their suitability as part of wound dressings, these NPs were combined with hyaluronan (HA)/collagen-based hydro- (HGs) and cryogels (CGs). Different HA and NP concentrations and loading techniques for NP incorporation were used. The NP release, gel morphology, and mechanical properties were studied. Generally, colonialization of the gels with macrophages resulted in high cell viability and proliferation. Furthermore, direct contact of the NPs to the cells reduced the level of nitric oxide (NO). The formation of multinucleated cells on the gels was low and further decreased by the NPs. For the HGs that produced the highest reduction in NO, extended ELISA studies showed reduced levels of the pro-inflammatory markers PGE2, IL-12 p40, TNF-α, and IL-6. Thus, HA/collagen-based gels containing KT NPs may represent a novel therapeutic approach for treating chronic wounds. Whether effects observed in vitro translate into a favorable profile on skin regeneration in vivo will require rigorous testing. |
| first_indexed | 2024-03-11T06:21:16Z |
| format | Article |
| id | doaj.art-888b1f2d03974365a5dd0075b7960ad4 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T06:21:16Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-888b1f2d03974365a5dd0075b7960ad42023-11-17T11:54:26ZengMDPI AGJournal of Functional Biomaterials2079-49832023-03-0114316010.3390/jfb14030160Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen HydrogelsNorbert Halfter0Eva Espinosa-Cano1Gloria María Pontes-Quero2Rosa Ana Ramírez-Jiménez3Christiane Heinemann4Stephanie Möller5Matthias Schnabelrauch6Hans-Peter Wiesmann7Vera Hintze8Maria Rosa Aguilar9Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, GermanyGroup of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainGroup of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainGroup of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainInstitute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, GermanyDepartment of Biomaterials, INNOVENT e. V., Prüssingstraße 27B, 07745 Jena, GermanyDepartment of Biomaterials, INNOVENT e. V., Prüssingstraße 27B, 07745 Jena, GermanyInstitute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, GermanyInstitute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, GermanyGroup of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, SpainCurrent limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanoparticles (KT NPs) can reduce pro-inflammatory markers of macrophages and increase anti-inflammatory cytokines. To assess their suitability as part of wound dressings, these NPs were combined with hyaluronan (HA)/collagen-based hydro- (HGs) and cryogels (CGs). Different HA and NP concentrations and loading techniques for NP incorporation were used. The NP release, gel morphology, and mechanical properties were studied. Generally, colonialization of the gels with macrophages resulted in high cell viability and proliferation. Furthermore, direct contact of the NPs to the cells reduced the level of nitric oxide (NO). The formation of multinucleated cells on the gels was low and further decreased by the NPs. For the HGs that produced the highest reduction in NO, extended ELISA studies showed reduced levels of the pro-inflammatory markers PGE2, IL-12 p40, TNF-α, and IL-6. Thus, HA/collagen-based gels containing KT NPs may represent a novel therapeutic approach for treating chronic wounds. Whether effects observed in vitro translate into a favorable profile on skin regeneration in vivo will require rigorous testing.https://www.mdpi.com/2079-4983/14/3/160ketoprofennanoparticleshyaluronancollagenhydrogelscryogels |
| spellingShingle | Norbert Halfter Eva Espinosa-Cano Gloria María Pontes-Quero Rosa Ana Ramírez-Jiménez Christiane Heinemann Stephanie Möller Matthias Schnabelrauch Hans-Peter Wiesmann Vera Hintze Maria Rosa Aguilar Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels Journal of Functional Biomaterials ketoprofen nanoparticles hyaluronan collagen hydrogels cryogels |
| title | Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels |
| title_full | Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels |
| title_fullStr | Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels |
| title_full_unstemmed | Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels |
| title_short | Ketoprofen-Based Polymer-Drug Nanoparticles Provide Anti-Inflammatory Properties to HA/Collagen Hydrogels |
| title_sort | ketoprofen based polymer drug nanoparticles provide anti inflammatory properties to ha collagen hydrogels |
| topic | ketoprofen nanoparticles hyaluronan collagen hydrogels cryogels |
| url | https://www.mdpi.com/2079-4983/14/3/160 |
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