Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling
As breast cancer is the leading cause of cancer-related deaths in women population worldwide, ongoing endeavor has been made for alternative regimens with improved efficacy but fewer adverse effects. Recently, active components from the spores of Ganoderma lucidum have attracted much attention for t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.01765/full |
| _version_ | 1818943326890491904 |
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| author | Jiyan Su Lu Su Lu Su Dan Li Dan Li Ou Shuai Yifan Zhang Huijia Liang Chunwei Jiao Zhanchi Xu Yong Lai Yizhen Xie Yizhen Xie |
| author_facet | Jiyan Su Lu Su Lu Su Dan Li Dan Li Ou Shuai Yifan Zhang Huijia Liang Chunwei Jiao Zhanchi Xu Yong Lai Yizhen Xie Yizhen Xie |
| author_sort | Jiyan Su |
| collection | DOAJ |
| description | As breast cancer is the leading cause of cancer-related deaths in women population worldwide, ongoing endeavor has been made for alternative regimens with improved efficacy but fewer adverse effects. Recently, active components from the spores of Ganoderma lucidum have attracted much attention for their versatile biological activities owing to the advance in sporoderm-breaking technology. Here, anticancer potential of an extract derived from the sporoderm-breaking spores of G. lucidum (ESG) was explored in a 4T1-breast cancer xenograft mice model. Results showed that ESG was able to suppress 4T1 tumor growth in vivo rather than in vitro. Flowcytometry analysis revealed that ESG could significantly increase both cytotoxic T cell (Tc) population and the ratio of Tc to helper T cell (Th) in peripheral blood of the tumor-bearing mouse; similar promotion on Tc was also found in tumor-infiltrating lymphocyte. Moreover, ESG evidently downregulated the two immune checkpoints, programmed cell death protein-1 (PD-1, in the spleen) and cytotoxic T lymphocyte antigen-4 (CTLA-4, in the tumor), suggesting that ESG could effectively restore the T cell paradigm by recovering the exhaustion status via suppressing the co-inhibitory checkpoints. By 16S rRNA gene sequence analysis on the fecal microbiota, it was found that ESG would remodeling the overall structure of the samples from tumor-bearing mice toward that of the normal counterparts, including 18 genera in 5 phyla, together with regulations on several genes that are responsible for signaling pathways involved in metabolism, cellular processes, and environmental information processing. Collectively, this study demonstrated that ESG would serve as a natural anticancer adjuvant via a restoration on the exhausted Tc, highlighting important clinical implications for the treatment of breast cancer. |
| first_indexed | 2024-12-20T07:25:33Z |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-20T07:25:33Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-888c130ff3164a8f890e4fb2aad645932022-12-21T19:48:32ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-07-01910.3389/fimmu.2018.01765330894Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota RemodelingJiyan Su0Lu Su1Lu Su2Dan Li3Dan Li4Ou Shuai5Yifan Zhang6Huijia Liang7Chunwei Jiao8Zhanchi Xu9Yong Lai10Yizhen Xie11Yizhen Xie12State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaSchool of Pharmacy and Chemistry, Dali University, Dali, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaSchool of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaSchool of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Pharmacy and Chemistry, Dali University, Dali, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou, ChinaGuangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, ChinaAs breast cancer is the leading cause of cancer-related deaths in women population worldwide, ongoing endeavor has been made for alternative regimens with improved efficacy but fewer adverse effects. Recently, active components from the spores of Ganoderma lucidum have attracted much attention for their versatile biological activities owing to the advance in sporoderm-breaking technology. Here, anticancer potential of an extract derived from the sporoderm-breaking spores of G. lucidum (ESG) was explored in a 4T1-breast cancer xenograft mice model. Results showed that ESG was able to suppress 4T1 tumor growth in vivo rather than in vitro. Flowcytometry analysis revealed that ESG could significantly increase both cytotoxic T cell (Tc) population and the ratio of Tc to helper T cell (Th) in peripheral blood of the tumor-bearing mouse; similar promotion on Tc was also found in tumor-infiltrating lymphocyte. Moreover, ESG evidently downregulated the two immune checkpoints, programmed cell death protein-1 (PD-1, in the spleen) and cytotoxic T lymphocyte antigen-4 (CTLA-4, in the tumor), suggesting that ESG could effectively restore the T cell paradigm by recovering the exhaustion status via suppressing the co-inhibitory checkpoints. By 16S rRNA gene sequence analysis on the fecal microbiota, it was found that ESG would remodeling the overall structure of the samples from tumor-bearing mice toward that of the normal counterparts, including 18 genera in 5 phyla, together with regulations on several genes that are responsible for signaling pathways involved in metabolism, cellular processes, and environmental information processing. Collectively, this study demonstrated that ESG would serve as a natural anticancer adjuvant via a restoration on the exhausted Tc, highlighting important clinical implications for the treatment of breast cancer.https://www.frontiersin.org/article/10.3389/fimmu.2018.01765/fullspore of Ganoderma lucidumcytotoxic T cellsexhaustionimmune checkpointsgut microbiota |
| spellingShingle | Jiyan Su Lu Su Lu Su Dan Li Dan Li Ou Shuai Yifan Zhang Huijia Liang Chunwei Jiao Zhanchi Xu Yong Lai Yizhen Xie Yizhen Xie Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling Frontiers in Immunology spore of Ganoderma lucidum cytotoxic T cells exhaustion immune checkpoints gut microbiota |
| title | Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling |
| title_full | Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling |
| title_fullStr | Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling |
| title_full_unstemmed | Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling |
| title_short | Antitumor Activity of Extract From the Sporoderm-Breaking Spore of Ganoderma lucidum: Restoration on Exhausted Cytotoxic T Cell With Gut Microbiota Remodeling |
| title_sort | antitumor activity of extract from the sporoderm breaking spore of ganoderma lucidum restoration on exhausted cytotoxic t cell with gut microbiota remodeling |
| topic | spore of Ganoderma lucidum cytotoxic T cells exhaustion immune checkpoints gut microbiota |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.01765/full |
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