The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton
<i>Verticillium dahliae</i> Kleb., the causal pathogen of vascular wilt, can seriously reduce the yield and quality of many crops, including cotton (<i>Gossypium hirsutum</i>). To control the harm caused by <i>V. dahliae</i>, considering the environmental pollutio...
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MDPI AG
2021-01-01
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| Series: | Pathogens |
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| author | Yalin Zhang Lihong Zhao Zili Feng Hongfu Guo Hongjie Feng Yuan Yuan Feng Wei Heqin Zhu |
| author_facet | Yalin Zhang Lihong Zhao Zili Feng Hongfu Guo Hongjie Feng Yuan Yuan Feng Wei Heqin Zhu |
| author_sort | Yalin Zhang |
| collection | DOAJ |
| description | <i>Verticillium dahliae</i> Kleb., the causal pathogen of vascular wilt, can seriously reduce the yield and quality of many crops, including cotton (<i>Gossypium hirsutum</i>). To control the harm caused by <i>V. dahliae</i>, considering the environmental pollution of chemical fungicides and their residues, the strategy of plant nutrition regulation is becoming increasingly important as an eco-friendly method for disease control. A new compound micronutrient fertilizer (CMF) found in our previous study could reduce the damage of cotton Verticillium wilt and increase yield. However, there is little information about the mode of action of CMF to control this disease. In the present study, we evaluated the role of CMF against <i>V. dahliae</i> and its mechanism of action in vitro and in vivo. In the laboratory tests, we observed that CMF could inhibit hyphal growth, microsclerotia germination, and reduce sporulation of <i>V. dahliae</i>. Further studies revealed that the biomass of <i>V. dahliae</i> in the root and hypocotyl of cotton seedlings treated with CMF were significantly reduced compared with the control, and these results could explain the decline in the disease index of cotton Verticillium wilt. Furthermore, those key genes involved in the phenylpropanoid metabolism pathway, resistance-related genes defense, and nitric oxide signaling pathway were induced in cotton root and hypocotyl tissue when treated with CMF. These results suggest that CMF is a multifaceted micronutrient fertilizer with roles in inhibiting the growth, development, and pathogenicity of <i>V. dahliae</i> and promoting cotton growth. |
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| last_indexed | 2024-03-09T04:22:04Z |
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| spelling | doaj.art-888cfac7e7e74edd9c85b71093932b8e2023-12-03T13:45:30ZengMDPI AGPathogens2076-08172021-01-011018110.3390/pathogens10010081The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on CottonYalin Zhang0Lihong Zhao1Zili Feng2Hongfu Guo3Hongjie Feng4Yuan Yuan5Feng Wei6Heqin Zhu7State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaPuyang City Academy of Agricultural Sciences, Puyang 457000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China<i>Verticillium dahliae</i> Kleb., the causal pathogen of vascular wilt, can seriously reduce the yield and quality of many crops, including cotton (<i>Gossypium hirsutum</i>). To control the harm caused by <i>V. dahliae</i>, considering the environmental pollution of chemical fungicides and their residues, the strategy of plant nutrition regulation is becoming increasingly important as an eco-friendly method for disease control. A new compound micronutrient fertilizer (CMF) found in our previous study could reduce the damage of cotton Verticillium wilt and increase yield. However, there is little information about the mode of action of CMF to control this disease. In the present study, we evaluated the role of CMF against <i>V. dahliae</i> and its mechanism of action in vitro and in vivo. In the laboratory tests, we observed that CMF could inhibit hyphal growth, microsclerotia germination, and reduce sporulation of <i>V. dahliae</i>. Further studies revealed that the biomass of <i>V. dahliae</i> in the root and hypocotyl of cotton seedlings treated with CMF were significantly reduced compared with the control, and these results could explain the decline in the disease index of cotton Verticillium wilt. Furthermore, those key genes involved in the phenylpropanoid metabolism pathway, resistance-related genes defense, and nitric oxide signaling pathway were induced in cotton root and hypocotyl tissue when treated with CMF. These results suggest that CMF is a multifaceted micronutrient fertilizer with roles in inhibiting the growth, development, and pathogenicity of <i>V. dahliae</i> and promoting cotton growth.https://www.mdpi.com/2076-0817/10/1/81cotton<i>Verticillium dahliae</i>pathogenicitymicronutrientprevention strategy |
| spellingShingle | Yalin Zhang Lihong Zhao Zili Feng Hongfu Guo Hongjie Feng Yuan Yuan Feng Wei Heqin Zhu The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton Pathogens cotton <i>Verticillium dahliae</i> pathogenicity micronutrient prevention strategy |
| title | The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton |
| title_full | The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton |
| title_fullStr | The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton |
| title_full_unstemmed | The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton |
| title_short | The Role of a New Compound Micronutrient Multifunctional Fertilizer against <i>Verticillium dahliae</i> on Cotton |
| title_sort | role of a new compound micronutrient multifunctional fertilizer against i verticillium dahliae i on cotton |
| topic | cotton <i>Verticillium dahliae</i> pathogenicity micronutrient prevention strategy |
| url | https://www.mdpi.com/2076-0817/10/1/81 |
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