Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor
Abstract Background Human adipose-derived stem cells (hADSCs) are capable of differentiating into many cells such as cardiac cells. Different types of inducers are used for cardiac cell differentiation, but this question still remains to be investigated, which one is the best. The aim of this paper...
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BioMedical Engineering OnLine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12938-017-0423-y |
| _version_ | 1818932759356243968 |
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| author | Zahra Bagheri-Hosseinabadi Parvin Salehinejad Seyed Alireza Mesbah-Namin |
| author_facet | Zahra Bagheri-Hosseinabadi Parvin Salehinejad Seyed Alireza Mesbah-Namin |
| author_sort | Zahra Bagheri-Hosseinabadi |
| collection | DOAJ |
| description | Abstract Background Human adipose-derived stem cells (hADSCs) are capable of differentiating into many cells such as cardiac cells. Different types of inducers are used for cardiac cell differentiation, but this question still remains to be investigated, which one is the best. The aim of this paper was to investigate the effect of combination of fibrin scaffold and trichostatin A (TSA), for differentiation of hADSCs into cardiomyocyte-like cells. Methods After approval of characteristics of hADSCs and fibrin scaffold, hADSCs were cultured in fibrin scaffold with 10 µM TSA for 72 h and kept in standard conditions for 4 weeks. QRT-PCR and immunostaining assay were performed for evaluating the expression pattern of special cardiac genes and proteins. Results In particular, our study showed that fibrin scaffold alongside TSA enhanced expression of the selected genes and proteins. Conclusions We concluded that the TSA alone or with fibrin scaffold can lead to the generation of cardiac like cells in a short period of time. |
| first_indexed | 2024-12-20T04:37:35Z |
| format | Article |
| id | doaj.art-888d60bb612848748baceba77c3930ec |
| institution | Directory Open Access Journal |
| issn | 1475-925X |
| language | English |
| last_indexed | 2024-12-20T04:37:35Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BioMedical Engineering OnLine |
| spelling | doaj.art-888d60bb612848748baceba77c3930ec2022-12-21T19:53:13ZengBMCBioMedical Engineering OnLine1475-925X2017-11-0116111210.1186/s12938-017-0423-yDifferentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitorZahra Bagheri-Hosseinabadi0Parvin Salehinejad1Seyed Alireza Mesbah-Namin2Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares UniversityDepartment of Anatomy, Afzalipour School of Medicine, Kerman University of Medical SciencesDepartment of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares UniversityAbstract Background Human adipose-derived stem cells (hADSCs) are capable of differentiating into many cells such as cardiac cells. Different types of inducers are used for cardiac cell differentiation, but this question still remains to be investigated, which one is the best. The aim of this paper was to investigate the effect of combination of fibrin scaffold and trichostatin A (TSA), for differentiation of hADSCs into cardiomyocyte-like cells. Methods After approval of characteristics of hADSCs and fibrin scaffold, hADSCs were cultured in fibrin scaffold with 10 µM TSA for 72 h and kept in standard conditions for 4 weeks. QRT-PCR and immunostaining assay were performed for evaluating the expression pattern of special cardiac genes and proteins. Results In particular, our study showed that fibrin scaffold alongside TSA enhanced expression of the selected genes and proteins. Conclusions We concluded that the TSA alone or with fibrin scaffold can lead to the generation of cardiac like cells in a short period of time.http://link.springer.com/article/10.1186/s12938-017-0423-yFibrin scaffoldDifferentiationHuman ADSCsCardiomyocyte-like cellsTSA |
| spellingShingle | Zahra Bagheri-Hosseinabadi Parvin Salehinejad Seyed Alireza Mesbah-Namin Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor BioMedical Engineering OnLine Fibrin scaffold Differentiation Human ADSCs Cardiomyocyte-like cells TSA |
| title | Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor |
| title_full | Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor |
| title_fullStr | Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor |
| title_full_unstemmed | Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor |
| title_short | Differentiation of human adipose-derived stem cells into cardiomyocyte-like cells in fibrin scaffold by a histone deacetylase inhibitor |
| title_sort | differentiation of human adipose derived stem cells into cardiomyocyte like cells in fibrin scaffold by a histone deacetylase inhibitor |
| topic | Fibrin scaffold Differentiation Human ADSCs Cardiomyocyte-like cells TSA |
| url | http://link.springer.com/article/10.1186/s12938-017-0423-y |
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