Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model
Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movem...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/7/1398 |
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| author | Marlena Zielińska-Górska Ewa Sawosz Malwina Sosnowska Anna Hotowy Marta Grodzik Konrad Górski Barbara Strojny-Cieślak Mateusz Wierzbicki André Chwalibog |
| author_facet | Marlena Zielińska-Górska Ewa Sawosz Malwina Sosnowska Anna Hotowy Marta Grodzik Konrad Górski Barbara Strojny-Cieślak Mateusz Wierzbicki André Chwalibog |
| author_sort | Marlena Zielińska-Górska |
| collection | DOAJ |
| description | Silver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movement without inhibiting their antibacterial properties. Considering that the human skin is often the first contact with antimicrobial agent, the aim of the study was to assess whether the application of the complex of AgNP and GO is biocompatible with the skin model in in vitro studies. The conducted tests were performed in accordance with the criteria set in OECD TG439. AgNP-GO complex did not influence the genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover, it showed a positive effect on the micro- and ultra-structure of the skin model. In conclusion, the synergistic effect of AgNP and GO as a complex can activate the process of epidermis renewal, which makes it suitable for use as a material for skin contact. |
| first_indexed | 2024-03-09T13:11:44Z |
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| id | doaj.art-888d6896abda45d9a7af833b80fc53e1 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T13:11:44Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-888d6896abda45d9a7af833b80fc53e12023-11-30T21:41:41ZengMDPI AGPharmaceutics1999-49232022-07-01147139810.3390/pharmaceutics14071398Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro ModelMarlena Zielińska-Górska0Ewa Sawosz1Malwina Sosnowska2Anna Hotowy3Marta Grodzik4Konrad Górski5Barbara Strojny-Cieślak6Mateusz Wierzbicki7André Chwalibog8Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandInterdisciplinary Division for Energy Analyses, National Centre for Nuclear Research, 05-400 Otwock, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkSilver nanoparticles (AgNP) can migrate to tissues and cells of the body, as well as to agglomerate, which reduces the effectiveness of their use for the antimicrobial protection of the skin. Graphene oxide (GO), with a super-thin flake structure, can be a carrier of AgNP that stabilizes their movement without inhibiting their antibacterial properties. Considering that the human skin is often the first contact with antimicrobial agent, the aim of the study was to assess whether the application of the complex of AgNP and GO is biocompatible with the skin model in in vitro studies. The conducted tests were performed in accordance with the criteria set in OECD TG439. AgNP-GO complex did not influence the genotoxicity and metabolism of the tissue. Furthermore, the complex reduced the pro-inflammatory properties of AgNP by reducing expression of IP-10 (interferon gamma-induced protein 10), IL-3 (interleukin 3), and IL-4 (interleukin 4) as well as MIP1β (macrophage inflammatory protein 1β) expressed in the GO group. Moreover, it showed a positive effect on the micro- and ultra-structure of the skin model. In conclusion, the synergistic effect of AgNP and GO as a complex can activate the process of epidermis renewal, which makes it suitable for use as a material for skin contact.https://www.mdpi.com/1999-4923/14/7/1398silver nanoparticlesgraphene oxidebiocompatibility3D epidermismorphologycytokines |
| spellingShingle | Marlena Zielińska-Górska Ewa Sawosz Malwina Sosnowska Anna Hotowy Marta Grodzik Konrad Górski Barbara Strojny-Cieślak Mateusz Wierzbicki André Chwalibog Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model Pharmaceutics silver nanoparticles graphene oxide biocompatibility 3D epidermis morphology cytokines |
| title | Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model |
| title_full | Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model |
| title_fullStr | Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model |
| title_full_unstemmed | Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model |
| title_short | Molecular Biocompatibility of a Silver Nanoparticle Complex with Graphene Oxide to Human Skin in a 3D Epidermis In Vitro Model |
| title_sort | molecular biocompatibility of a silver nanoparticle complex with graphene oxide to human skin in a 3d epidermis in vitro model |
| topic | silver nanoparticles graphene oxide biocompatibility 3D epidermis morphology cytokines |
| url | https://www.mdpi.com/1999-4923/14/7/1398 |
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